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Webinar: Improvement of AAV Productivity by Characterization of Agitation Sensitivity of Seed and Production Stages Using Biostat® RM and Ambr® Bioreactors
October 21 @ 8:00 am - 9:00 am PDT
Scaling up a process developed in shake flasks to manufacturing scale requires different agitation and may impact process performance. Shake flasks are typically used at early stage process development for production and seed cell growth but replaced with stirred tanks and rocked bags respectively as scale increases. Biostat® RM combined with Ambr® were used to map out the agitation design space of the both the seed and production systems to establish appropriate operating parameters for large-scale. A method for quantifying the level of cell aggregation was also developed to allow comparison between systems. This showed the importance of configuring agitation to maximise productivity. Overall, it was found that agitation had a significant effect on productivity and that different agitation rates are beneficial at different stages of the process. Appropriate agitation for Biostat® RM when used as a seed vessel had the potential to increase titre by 50% for stirred tank production. Optimising agitation rate during production in Biostat® RM could increase titre by several-fold.