Chromatography Ligand Comprising Domain C from Staphylococcus Aureus Protein A for Antibody Isolation
A U.S. Patent for a "Chromatography Ligand Comprising Domain C from Staphylococcus Aureus Protein A for Antibody Isolation" was awarded to Cytiva on December 29th, 2020. The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.