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Patents | Aspen Xchange

Patents

A U.S. Patent for a "Chromatography Ligand Comprising Domain C from Staphylococcus Aureus Protein A for Antibody Isolation" was awarded to Cytiva on December 29th, 2020. The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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A U.S. Patent for "Nanoscale Coatings for Encapsulation of Biological Entities" was awarded to The Regents of the University of California on December 22nd, 2020. The present invention discloses  methods, systems, and devices for encapsulating biological entities with preservation of their biological activity. In one aspect, a method of encapsulating a biological entity includes templating a biocompatible material onto a biological structure to form a coating structure enclosing the biological structure, the coating structure having a size in the nanometer range, in which the coated biological structure preserves its biological activity within the coating structure. In some implementations of the method, the biological structure includes a virus and the biocompatible material includes silica.
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A U.S. Patent for a "Cell Survival Rate Determining Device" was awarded to Azbil on December 22nd, 2020. The present invention is a cell survival rate determining device includes a survival rate calculation unit calculating the survival rate of cells in a cell population based on a peak wavelength giving a peak intensity of autofluorescence emitted by the cell population and a peak wavelength of excitation light giving the peak intensity of autofluorescence; and a determination unit determining whether the calculated survival rate of cells is reliable or not based on an amount of a shift and a direction of the shift per unit time in the peak wavelength of autofluorescence and an amount of a shift and a direction of the shift per unit time in the peak wavelength of excitation light.
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A U.S. Patent for an "Electromagnetic Rotary Drive" was awarded to Levitronix on December 22nd, 2020. The present invention is an electromagnetic rotary drive that includes a magnetically contactlessly drivable rotor free of coils, and a stator configured as a bearing and drive stator configured to drive the rotor magnetically and contactlessly about an axis of rotation. The rotor is capable of being supported magnetically contactlessly with respect to the stator in an operating state. The stator includes an upper stator part having a plurality of pronounced upper poles configured to carry upper windings and a lower stator part having a plurality of pronounced lower poles configured to carry lower windings. The upper stator part and the lower stator part are arranged spaced apart from one another with respect to an axial direction.
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A U.S. Patent for a "Computer Implemented Method for Creating a Fermentation Model" was awarded to Bayer on December 22nd, 2020. The present invention is a computer-implemented method for model-based estimation of the state of a bioreaction with an organism in a bioreactor comprising: creating a model of a bioreaction with an organism, said model including a matrix L, kinetics of macroreactions and model parameters of calculated values, which comprises the following steps: a. defining selected metabolic pathways of the organism, their properties of stoichiometry and reversibility as background knowledge and calculating elementary modes from this input; b. combining the elementary modes from a) in a matrix K, wherein the elementary modes combine the metabolic pathways from a) in macroreactions and the matrix K contains the stoichiometry...
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A U.S. Patent for "Aseptic Coupling Devices" was awarded to CPC on December 22nd, 2020. The present invention is a method for creating an aseptic connection, the method comprising: inserting a front portion of a first aseptic coupling device into a second aseptic coupling device by moving the first and second aseptic coupling devices toward each other along a centerline of the first and second aseptic coupling devices to thereby cause front face portions of the first and second aseptic coupling devices to engage together, each of the first and second aseptic coupling devices defining a fluid passage therethrough along the centerline and comprising: a seal member positioned in a window formed by the respective coupling; and a membrane coupled to a front surface of the respective coupling to cover the seal member...
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A U.S. Patent for "Configurable Methods and Systems of Growing and Harvesting Cells in a Hollow Fiber Bioreactor System" was awarded to Terumo BCT on December 22nd, 2020. The present invention generally relates to methods and systems for configuring settings of a cell expansion system including a bioreactor. Through a user interface, a user may configure display settings, system settings, and settings associated with protocols for loading, growing and/or harvesting cells. In configuring settings for protocols and associated processes, a diagram view or window of the cell expansion system is displayed in embodiments. The diagram view displays the process settings as graphical user interface elements. Settings available for configuration are enabled for selection in the diagram view. 
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A U.S. Patent for "Vessel for Growth of Biological Entities" was awarded to Corning on December 22nd, 2020. The present invention is a 5-liter bioreactor vessel that enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5.times. the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.
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A U.S. Patent for "Methods and Systems for Assessing Flow Cell Cleanliness" was awarded to Abbott on December 15th, 2020. The present invention is a flow cytometer comprising: a flow cell; a light emitter; a pre-flow cell detector; a post-flow cell detector; and a computing device having instructions that, when executed by the computing device, cause the flow cytometer to perform the steps of: a) emit a light beam from the light emitter through the flow cell; b) measure the intensity of the light beam at the pre-flow cell detector and the post-flow cell detector; c) calculate a ratio of the measured intensities of the light beam at the pre- and post-flow cell detectors; and d) compare the calculated ratio to a reference ratio stored on the computing device to assess the cleanliness of the flow cell...
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A U.S. Patent for "Configurable Methods and Systems of Growing and Harvesting Cells in a Hollow Fiber Bioreactor System" was awarded to Terumo BCT on December 22nd, 2020. The present invention generally relates to methods and systems for configuring settings of a cell expansion system including a bioreactor. Through a user interface, a user may configure display settings, system settings, and settings associated with protocols for loading, growing and/or harvesting cells. In configuring settings for protocols and associated processes, a diagram view or window of the cell expansion system is displayed in embodiments. The diagram view displays the process settings as graphical user interface elements. Settings available for configuration are enabled for selection in the diagram view. 
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A U.S. Patent for "Recombinant Host Cell Engineered to Overexpress Helper Proteins" was awarded to Boehringer Ingelheim on December 15th, 2020. The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.
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A U.S. Patent for "Purification of Biological Molecules" was awarded to MilliporeSigma on December 15th, 2020. The present invention is a continuous process for the purification of a target molecule comprising the steps of: a) providing a sample comprising the target molecule and one or more impurities; b) adding at least one precipitant to the sample and removing one or more impurities, thereby to recover a clarified sample; c) subjecting the clarified sample from step (b) to a bind and elute chromatography step comprising at least two separation units, thereby to obtain an eluate comprising the target molecule; and d) subjecting the eluate to flow-through purification comprising use of two or more media, one of which is activated carbon and the other(s) are selected from anion-exchange chromatography media...
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A U.S. Patent for "Purification of Biological Molecules" was awarded to MilliporeSigma on December 15th, 2020. The present invention is a continuous process for the purification of a target molecule comprising the steps of: a) providing a sample comprising the target molecule and one or more impurities; b) adding at least one precipitant to the sample and removing one or more impurities, thereby to recover a clarified sample; c) subjecting the clarified sample from step (b) to a bind and elute chromatography step comprising at least two separation units, thereby to obtain an eluate comprising the target molecule; and d) subjecting the eluate to flow-through purification comprising use of two or more media, one of which is activated carbon and the other(s) are selected from anion-exchange chromatography media...
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A U.S. Patent for "Purification of Biological Molecules" was awarded to MilliporeSigma on December 15th, 2020. The present invention is a continuous process for the purification of a target molecule comprising the steps of: a) providing a sample comprising the target molecule and one or more impurities; b) adding at least one precipitant to the sample and removing one or more impurities, thereby to recover a clarified sample; c) subjecting the clarified sample from step (b) to a bind and elute chromatography step comprising at least two separation units, thereby to obtain an eluate comprising the target molecule; and d) subjecting the eluate to flow-through purification comprising use of two or more media, one of which is activated carbon and the other(s) are selected from anion-exchange chromatography media...
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A U.S. Patent for a "Probe Assembly" was awarded to Global Life Sciences Solutions on December 8th, 2020. The present invention is a probe assembly for inserting a probe into a flexible or semi-rigid object is provided. The probe assembly includes an aseptic connector for coupling to the object, a probe sheath that extends longitudinally from the aseptic connector, and an actuator for deploying the probe within the object. The probe sheath has a plurality of concentric sheath elements and an inner longitudinal volume that is configured to permit longitudinal movement of the probe within the probe sheath. The actuator moves the probe longitudinally within the probe sheath by telescoping a first sheath element of the plurality within another sheath element of the plurality such that the probe is moved to a position...
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A U.S. Patent for "Removal of Microorganisms from Cell Culture Media " was awarded to MilliporeSigma on December 8th, 2020. The present invention is an asymmetric, porous polyethersulfone membrane having a surface modified with a polymer consisting essentially of randomly arranged and crosslinked monomers of diacetone acrylamide and a non-acrylamide cross-linkable difunctional monomer, wherein the non-acrylamide cross-linkable difunctional monomer is polyethylene glycol diacrylate.
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A U.S. Patent for "Heatseal Sampling" was awarded to Global Life Sciences Solutions on December 8th, 2020. The present invention is a device for taking a sample from a liquid cell culture in a bioreactor wherein said device comprises: (i) a hollow body comprising outer walls that define a fluid path therethrough wherein said outer walls are at least partly comprised of a sealable material, wherein said fluid path begins at an upstream end of said hollow body, through said hollow body, and ends at a downstream end of said hollow body, and wherein said upstream and downstream ends are adapted to fluidly connect said fluid path with an interior of said bioreactor, wherein the liquid cell culture flows from the interior of said bioreactor into the fluid path via the upstream end and flows back to the interior of said bioreactor...
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A U.S. Patent for a "Bioprocess Mixer" was awarded to Global Life Sciences Solutions on December 8th, 2020. The present invention is a bioprocess mixer, comprising: a support vessel comprising at least one side wall and a bottom wall, said walls defining a support vessel inner volume, and at least a first and a second magnetic impeller drive unit; and a flexible bag adapted to fit inside said support vessel inner volume, said bag having at least one bag side wall, a bag bottom wall and a bag top wall defining a bag inner volume, with at least a first and a second magnetic impeller rotatably attached to one or more bag wall in said bag inner volume, wherein said support vessel inner volume has a length-to-width ratio of at least 1.5 for facilitating movement of the bioprocess mixer...
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A U.S. Patent for "Virus Purification" was awarded to CC Biotech on December 1st, 2020. The present invention is a method for purifying a virus particle comprising applying a sample comprising a virus in a two part aqueous solvent comprising a carbohydrate and a salt into a spiral tubing in a countercurrent chromatography rotor, flowing a fluid phase through said tubing, and exposing said rotor to planetary motion to separate said virus from said sample. 
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A U.S. Patent for a "Chromatography Medium" was awarded to Puridify on December 1st, 2020. The present invention is a process for preparing a functionalised polymeric chromatography medium, which process comprises (I) providing a stack of two or more non-woven sheets stacked one on top of the other, each said sheet consisting essentially of one or more polymer nanofibres, (II) simultaneously heating and pressing the stack of sheets to fuse points of contact between the nanofibres of adjacent sheets to form a pressed and heated product, and (III) contacting the pressed and heated product of step (II) in a batchwise fashion in a plurality of batches with a respective batch reagent associated with each batch of the plurality of batches, said respective batch reagent being same or different in the plurality of batches...
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A U.S. Patent for a "Fluid Mixing System" was awarded to Thermo Fisher on December 1st, 2020. The present invention is a fluid mixing system that includes a flexible bag having a first end, an opposing second end, and an interior surface bounding a compartment. A first rotational assembly includes a first casing mounted to the first end of the flexible bag and a first hub rotatably mounted to the first casing. A second rotational assembly includes a second casing mounted to the second end of the flexible bag and a second hub rotatably mounted to the second casing. An elongated member has a first end coupled with the first hub and an opposing second end coupled with the second hub, the connector being flexible and having a uniform flexibility along its entire length. A first impeller is mounted to the connector.
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A U.S. Patent for a "Method of Manufacturing a Leaktight Container" was awarded to Sartorius on November 24th, 2020. The present invention is a method for manufacturing a leaktight assembly by providing a bag, a connection system including a connector adapted for mechanical connection to a complementary connector of a sterile chamber in order to establish sterile communication between the bag and the chamber, the connector including a portion for attachment to the bag, the connection system further including an overmolding molded over the attachment portion, the manufacturing method further including the steps of positioning the wall of the bag facing the overmolding at a first access opening of the bag, the attachment portion surrounding a space for passage between the bag and the chamber...
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A U.S. Patent for a "Container with Film Sparger" was awarded to Thermo Fisher on November 24th, 2020. The present invention is a container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface. The bag has a bottom end wall that includes a first sparger and a second sparger. The first sparger and the second sparger each have a flexible first sheet overlying a flexible second sheet, the first sheet and the second sheet being secured together so as to form a sparging area bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the sparging area being gas permeable so that gas can pass from the sparging area, through the first sheet, and into the chamber.
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A U.S. Patent for a "Closed Fluid Receiving and Sampling Container" was awarded to Sani-Tech West on November 24th, 2020. The present invention is a closed fluid receiving and sampling container that enables transfer of valuable reaction liquid to a receptacle without risking loss of sterility. The sampling container has a dip tube subassembly with a shorter inlet tube bent towards the wall of the receptacle to prevent or reduce foaming, and a longer outlet tube used to drain the waste liquid once the magnetic beads are trapped by the magnet. The dip tube subassembly is injection molded in one piece and provides a sealed lid also with a vent tube therethrough to enable filling and draining the receptacle without removing the lid, thus keeping the process aseptic.
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A U.S. Patent for "Bioreactor Outlet Air Conditioning Systems and Associated Methods" was awarded to Sartorius on November 24th, 2020. The present invention is a bioreactor, comprising: a disposable bioreactor vessel comprising a vessel body and a lid having a plane and an integral outlet fluid flow path that is integral with and oriented in the same plane as the lid, the integral outlet fluid flow path comprising a conduit enclosed in the lid that has a first end in fluid communication with a chamber of the vessel body and a second end connected to an outlet line connected to the lid to pass evaporated outlet fluid from the chamber, the lid further comprising a port which defines a through hole in the lid; a groove that shares a boundary wall with the conduit such that heat transfers between the conduit and the groove via the boundary wall... 
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A U.S. Patent for a "High Density Fill and Draw Fermentation Process" was awarded to CMC Biologics on November 24th, 2020. The present invention relates to a high cell density fermentation fill and draw process for producing a product in a bioreactor system wherein during the fermentation, medium comprising impurities is removed via the impurity filter unit while new fresh medium is added to the cell culture vessel to replenish consumed nutrients and expelled medium.
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A U.S. Patent for "Manganese Supplementation for Control of Glycosylation in Mammalian Cell Culture Process" was awarded to Biogen on November 17th, 2020. The present inventionis a method for achieving a predetermined glycosylation profile of an anti-.alpha.4-integrin antibody, an interferon beta-1a polypeptide, or a rFVIIIFc, the method comprising: adding manganese to a cell culture at a concentration that falls within a target manganese concentration range, wherein the cell culture comprises host cells producing the anti-.alpha.4-integrin antibody, interferon beta-1a polypeptide, or rFVIIIFc, and if the manganese concentration in the cell culture is below the target manganese concentration range, the cell culture is supplemented with manganese, wherein the target manganese concentration range in the cell culture...
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A U.S. Patent for "Methods for Cryopreservation of Biological Materials" was awarded to Asymptote on November 17th, 2020. The present invention is an apparatus for performing ice nucleation, the apparatus comprising: a housing, the housing comprising a first material layer forming a permeable housing wall and a second material layer opposing the first material layer, the first material layer and the second material layer defining a cavity therebetween; an ice nucleating material encapsulated within the cavity; and a multiwell plate, the multiwell plate comprising: a plurality of wells, and a plurality of integrated housings, wherein each integrated housing of the plurality of integrated housings is integrated into a respective well of the plurality of wells, and wherein the housing is one of the plurality of integrated housings integrated into a respective well...
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A U.S. Patent for a "Bioreactor System and Single Use Cell Culture Container for Use Therein" was awarded to Scinus Cell Expansion on November 17th, 2020. The present invention is a bioreactor system for culturing cells, the system including: a variable volume container; wherein the variable volume container comprises: a flexible outer wall defining a cell-culture chamber; a first edge for removable attachment to a support of an agitator unit of the bioreactor system; a second edge, parallel to and spaced from the first edge, for removable attachment to one of a cylindrical roller and a support of the agitator unit; a minimum volume area of the container being defined adjacent to the first edge; a first fluid port in the minimum volume area for adding and removing cells and cell culture medium; a second fluid port in the minimum volume area, the second fluid port being connectable.
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A U.S. Patent for a "Disposable Container, Mixing System and Packaging" was awarded to Global Life Sciences Solutions on November 17th, 2020. The present invention is a bioreactor system comprising: a vessel for housing biomaterials for processing, the vessel comprising a flexible material defining a flexible chamber having a chamber wall that comprises at least three orifices and a tubing section extending from each orifice into the chamber; a mixing system positioned within the chamber, the mixing system comprising an agitator for imparting motion and mixing to the contents of the vessel such that biomaterials contained within the chamber are mixed and gas bubble circulation is increased, the agitator comprising: a base welded to the flexible material at a base section of the chamber, a shaft moveably mounted in the base and extending from the base...
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A U.S. Patent for a "Sensor Interface for Single Use Containers" was awarded to Emerson on November 17th, 2020. The present invention is a single-use sensor interface for coupling a single-use container to a re-usable sensing instrument is provided. The single-use sensor interface includes a polymeric flange couplable to a wall of the single-use container. The polymeric flange has a sidewall and an interface portion configured to receive a deflectable polymeric diaphragm. A deflectable polymeric diaphragm is coupled to the interface portion of the polymeric flange. An instrument coupling portion is sealingly coupled to the polymeric flange. The instrument coupling portion is configured to couple to the re-usable sensing instrument. Another single-use sensor interface for coupling a single-use container to a re-usable sensing instrument...
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A U.S. Patent for "Aseptic Coupling Devices" was awarded to CPC on November 17th, 2020. The present invention is a first aseptic coupling device for coupling to a second aseptic coupling device, the first aseptic coupling device comprising: a first main body defining a first face and a first fluid passage therethrough; a first sealing member received at least partially within the first main body; a first membrane coupled to the first face and covering the first sealing member; and a locking ring including a locking feature, the locking ring being positionable relative to the first main body in an initial position in which: the locking ring allows engagement of the first aseptic coupling device to the second aseptic coupling device, and the locking ring is rotatable about the first main body in a forward direction...
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A U.S. Patent for a "Feed Mixing Device and Its Use" was awarded to Roche on November 17th, 2020. The present invention is a device for adding a mixed feed solution to a cell cultivation vessel comprising viable mammalian cells, the device is made of sterilizable material and comprises separate inlets for at least two feed solutions, a chamber for mixing the feed solutions to obtain the mixed feed solution, and a single outlet for directly adding the mixed feed solution to the cell cultivation vessel, whereby at least one feed solution is an acidic feed solution and has a pH value of less than pH 6.5, and at least one feed solution is an alkaline feed solution and has a pH value of more than pH 7.5, the chamber for mixing the feed solutions has a volume of from 0.8 ml to 1.2 ml per liter of cultivation medium in the cultivation vessel. 
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A U.S. Patent for "Parallel Separation System" was awarded to Cytiva on November 10th, 2020. The present invention is a method in a separation system comprising the steps of: providing: (i) a plurality of parallel fluid paths, each fluid path comprising a separation module and a sensor; wherein, each sensor is a disposable probe independently positioned within each parallel fluid path downstream of the respective separation module; (ii) an inlet fluid path and an outlet fluid path, both being in fluidic connection to the separation modules; (ii) a system sensor positioned in the outlet fluid path; and (iv) a control system; initiating operation of the separation modules simultaneously, and measuring a characteristic fluid property of each separation module via the sensors downstream of the separation modules...
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A U.S. Patent for "Reusable Aseptic Fluid Couplings" was awarded to CPC on November 10th, 2020. The present invention is a fluid coupling comprising: a housing defining: (i) a first longitudinal axis and (ii) a second longitudinal axis that is parallel to the first longitudinal axis; a connection member configured to releasably couple a mating coupling to the fluid coupling, the connection member movably coupled to the housing and movable between a first position in which the connection member is coaxial with the first longitudinal axis and a second position in which the connection member is coaxial with the second longitudinal axis; a valve body coupled with the housing and coaxial with the second longitudinal axis, the valve body including a spring-loaded valve sleeve that is movable along the second longitudinal axis to open...
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A U.S. Patent for "Production of Recombinant von Willebrand Factor in a Bioreactor" was awarded to CSL on November 10th, 2020. The present invention is a process for manufacturing a recombinant von Willebrand factor (vWF), comprising (a) culturing a cell culture of host cells in a bioreactor in a cell culture medium, wherein the host cells produce recombinant vWF which is secreted into the cell culture medium and wherein the secreted recombinant vWF in the cell culture medium comprises vWF multimers of different sire, wherein at least one cell culture medium component is fed to the cell culture medium, and (b) pumping the cell culture comprising the host cells, the recombinant vWF, and the cell culture medium over a separation system having a molecular weight cut off size of about 750,000 Da.
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A U.S. Patent for an "Air Accordion Bioreactor" was awarded to the Arizona Board of Regents on November 10th, 2020. The present invention is a bioreactor comprising: (a) a container for holding fluid comprising a first sheet and a second sheet, wherein the second sheet is disposed adjacent to the first sheet and the first and second sheets are sealed along a first longitudinal edge, a second longitudinal edge, a first horizontal edge, and a second horizontal edge, and comprising at least one opening in the second horizontal edge; (b) a support structure comprising at least two vertical supports and at least two horizontal supports, wherein two or more portions of the container of part (a) are alternately positioned in front of and behind the at least two horizontal supports, thereby orienting the two or more portions of the container at alternating angles...
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A U.S. Patent for "Cell Culture Compositions with Antioxidants and Methods for Polypeptide Production" was awarded to Genentech on November 10th, 2020. The present invention is a method of producing a recombinant polypeptide composition with reduced color intensity, comprising the steps of: culturing a cell comprising a nucleic acid encoding the recombinant polypeptide in a cell culture medium, wherein the cell culture medium comprises aminoguanidine; producing the recombinant polypeptide; wherein the cell culture medium comprising the aminoguanidine reduces the color intensity of a composition comprising the recombinant polypeptide produced by the cell as compared to a composition comprising the recombinant polypeptide produced by the cell cultured in a cell culture medium...
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A U.S. Patent for "Containers and Systems for Processing a Fluid" was awarded to Thermo Fisher on November 30th, 2020. The present invention is a container for use in processing a fluid, the container comprising: a first wall and an opposing second wall that extend between a first end and an opposing second end, a first fluid flow path being bounded between the first wall and the second wall; an inlet formed at the first end and communicating with the first fluid flow path; an outlet formed at the second end and communicating with the first fluid flow path, the first fluid path being open between the inlet and the outlet; wherein the first wall comprises: a primary wall portion having a first opening extending therethrough in alignment with the first fluid flow path, the first opening being spaced apart from the inlet and the outlet...
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A U.S. Patent for "Methods and Compositions Comprising Purified Recombinant Polypeptides" was awarded to Genetech on November 3rd, 2020. The present incention is a method of purifying a recombinant antibody produced in Chinese hamster ovary host cells and having phospholipase B-like 2 (PLBL2) levels greater than 100 ng/mg, wherein the method comprises performing a hydrophobic interaction chromatography (HIC) step and provides a purified preparation comprising the recombinant antibody and a residual amount of hamster PLBL2, and wherein the residual amount of hamster PLBL2 is less than 20 ng/mg. 
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A U.S. Patent for a "Disposable Bioreactor System" was awarded to Thermo Fisher on November 3rd, 2020. The present invention is an apparatus comprising: a hollow toroidal one-piece polymeric bioreactor vessel comprising: at least one port configured to receive at least one sensor to monitor a bioprocess carried out in said hollow toroidal one-piece polymeric bioreactor vessel; textured surface arranged on an inner surface of the hollow toroidal one-piece polymeric bioreactor vessel, wherein a sinusoidal shape pattern of the textured surface comprises a plurality of upstanding protuberances; and a multitude of tubes coupled to the inner surface and extending through the plurality of upstanding protuberances of the textured surface, wherein the multitude of tubes are configured for sparging gas...
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A U.S. Patent for a "Process for Manufacturing Factor VIII Having an Improved Ratio of FVIII:C/FVIII/AG" was awarded to Octapharma on November 3rd, 2020. The present invention is a process for manufacturing of a Factor VIII product having a ratio of FVIII:C/FVIII:Ag of at least 0.7 in the Factor VIII product by using chromatography wherein at least one chromatographic step is performed by means of employing; An affinity chromatography resin having an affinity for specifically binding of Factor VIII which is effected by an affinity ligand which is immobilised on the affinity chromatography resin, said affinity ligand is a 13 kD yeast derived Fab antibody fragment directed to the Factor VIII molecule. An anionic chromatography resin. A size exclusion resin...
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A U.S. Patent for "Regulated Vacuum Off Gassing of Gas Filter for Fluid Processing System and Related Methods" was awarded to Thermo Fisher on November 3rd, 2020. The present invention is a method for filtering a gas, the method comprising: delivering a gas under a positive pressure into a compartment of a gas filter assembly; applying a partial vacuum to the gas filter assembly so that the partial vacuum assists in drawing the gas delivered into the compartment under the positive pressure through a porous filter body of the gas filter assembly that is at least partially disposed within the compartment of the gas filter assembly; regulating the application of the partial vacuum based on a pressure reading of the gas upstream of the gas filter assembly...
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A U.S. Patent for "Virus Purification" was awarded to Kentucky Bioprocessing on November 3rd, 2020. The present invention is a method for purifying at least one virus harvested from a source organism, comprising: removing cellular debris from the virus by passing the at least one virus through a tangential flow filtration apparatus comprising a porous ceramic filter; concentrating the virus; and performing separations comprising sub-steps of separating host cell contaminants from the virus, subjecting the virus to affinity chromatography to elute the virus, and subjecting the virus to multi-modal chromatography to separate residual impurities from the virus based on size differences between the impurities as retentate and the virus as permeate. 
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A U.S. Patent for "Pellets Used in Cell Culture and Methods of Making Thereof" was awarded to Thermo Fisher on November 3rd, 2020. The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to handle either before reconstitution...
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A U.S. Patent for "Multimodal Anion Exchange Matrices" was awarded to Cytiva on October 27th, 2020. The present invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R.sub.1-L.sub.1-N(R.sub.3)-L.sub.2-R, immobilized on a support, wherein R.sub.1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L.sub.1 is either a methylene group or a covalent bond; R.sub.2 is a five-or six-membered, substituted or non-substituted ring structure; L.sub.2 is either a methylene group or a covalent bond; R.sub.3 is a methyl group; and wherein if R.sub.1 is a hydroxyethyl group and L.sub.1 is a covalent bond, R.sub.2 is a substituted aromatic ring structure or a substituted or non-substituted...
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A U.S. Patent for a "Cell Line Screening Method" was awarded to Larix Bioscience on October 27th, 2020. The present invention provides a novel cell line development method useful to screen for recombinant protein production. The method utilizes a membrane-anchored reporter or an intracellular reporter residing in the expression vector for a gene of interest to facilitate initial cell selection by FACS or MACS. A switching mechanism can be used to delete the reporter from the chromosome by providing an appropriate DNA recombinase, which turns the selected cells into production cells that secrete the protein of interest without co-expression of the reporter.
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A U.S. Patent for "Devices, Systems and Methods for Zone Sterilization" was awarded to Burkert on October 27th, 2020. The present invention is a gas transfer system including a housing, a pressurized gas canister held by the housing, a first passageway in fluid communication with the pressurized gas canister and configured to supply pressurized pre-sterilization gas from the pressurized gas canister to a fluid flow component, a gas discharge canister held by the housing; and a second passageway in fluid communication with the gas discharge canister and configured to supply post soak gas from the fluid flow component to the gas discharge canister. A humidification system or device is included for the sterile humidification of sterilizing gas. 
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A U.S. Patent for a "Large Scale Acoustic Separation Device" was awarded to FloDesign Sonics on October 27th, 2020. The present invention is a method for separating a secondary fluid or particulate from a mixture with a host fluid, comprising: placing the mixture in an acoustophoretic device that comprises: an acoustic chamber; at least one ultrasonic transducer coupled to the acoustic chamber and configured to generate a multi-dimensional acoustic standing wave in the acoustic chamber; a reflector across the acoustic chamber from the at least one ultrasonic transducer; and an interior volume of at least 40 cubic inches; exciting the at least one transducer in a higher order mode to generate the multi-dimensional acoustic standing wave ... Learn More

A U.S. Patent for "Immunoglobulin-Binding Proteins and Their Use in Affinity Purification" was awarded to Navigo Proteins on October 20th, 2020. The present invention relates to non-natural binding proteins comprising one or more non-natural immunoglobulin (Ig) binding domains wherein at least one non-natural lg-binding domain comprises the amino acid sequence X1 X2X3XiXsX5X7 XsQQX11AFYX1sX15LX1 sX19PX21 LX23X24X2sQRX28X2gf IQSLKDDPSXio SXi2Xi3Xi4LXi5EAXigKLXs2Xs3Xs4QXs5PX. The disclosure also relates to compositions such as affinity matrices comprising the non-natural Ig-binding proteins of the invention. Learn More

A U.S. Patent for "Light Emitting Diode Photobioreactors and Methods of Use" was awarded to Avespa on October 20th, 2020. The present invention is a photobioreactor system and a process for its use, whereby water and nutrients from multiple sources are balanced (mixed using aeration) to the specific requirements of the particular photosynthetic organism strain used, sterilized, further mixed to balance the system and seeded with the photosynthetic microorganism, e.g. microalgae (dilution of a concentrated stock or added to an existing algal biomass). In accordance with such an embodiment, the algal biomass is then grown for a most efficient number of hours in a totally controlled environment... Learn More

A U.S. Patent for a “Mixing Vessel with Locking Assembly for Locking a Mixing Assembly in Storage Position and Mixing Impeller with Central Disc-Like Member” was awarded to Sartorius on October 20th, 2020. The present invention is a mixing vessel for accommodating components to be mixed has a container with at least one mounting depression in a side wall of the container. The mounting depression is adapted so that a mixing impeller housing of a mixing impeller is at least partly insertable, in which at least one magnet is housed for being magnetically connectable to a drive device to be driven. A locking assembly is attachable to the mounting depression from outside for locking the mixing impeller in a storage position… Learn More

A U.S. Patent for “Reactor Surface Finish Remediation” was awarded to Lonza on October 20th, 2020. The present invention is biopharmaceutical processing equipment, comprising: a vessel defining a volume for receiving a proteinaceous processing material, the vessel having an interior reactor surface that is configured to contact proteinaceous processing material when contained within the volume of the vessel, the biopharmaceutical processing equipment further comprising a clean-in-place (CIP) apparatus configured to clean the surface, and wherein the interior reactor surface is made from stainless steel and has a pre-commissioning surface roughness of greater than or equal to about 25 Ra Max. Learn More

A U.S. Patent for a "Method of Thermally Disinfecting a Centrifuge" was awarded to GEA on October 13th, 2020. The present invention is a method for thermally disinfecting a centrifuge by reducing a quantity of germs in the centrifuge, the method comprising: reducing the quantity of gems in the centrifuge using vapor that is not pressurized or which is at atmospheric pressure, wherein the centrifuge has a rotatable drum and is configured to centrifugally processing a product, wherein the vapor is passed through a feed, distributor ducts, a centrifuging chamber, a separating disk stack, and an outlet of the centrifuge, wherein, while the vapor is passed through the centrifuge, the drum is rotated. Learn More

A U.S. Patent for a "Chromatography Membranes, Devices Containing Them, and Methods of Use Thereof " was awarded to MilliporeSigma on October 13th, 2020. The present invention are fluid treatment devices for use in tangential flow filtration, comprising a housing unit and a composite material, wherein the composite material comprises: a support member comprising a plurality of pores extending through the support member; and a non-self-supporting macroporous cross-linked gel comprising macropores having an average size of 10 nm to 3000 nm, said macroporous gel being located in the pores of the support member. Learn More

A U.S. Patent for "Hydrophilic Filtration During the Manufacture of Vaccine Adjuvants" was awarded to Novartis on October 13th, 2020. The present invention is an improved method for the manufacture of an oil-in water emulsion involves three procedures: (i) preparation of a preliminary emulsion; (ii) micro fluidization of the preliminary emulsion to reduce its droplet size; and (iii) filtration, of the microfluidized emulsion through a hydrophilic membrane. The emulsions are useful as vaccine adjuvants.
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A U.S. Patent for a "Plunger Pumping Arrangement for a Hollow Fiber Filter" was awarded to Repligen on October 13th, 2020. The present invention is a fluid filtration assembly that includes a filter housing having a first end for fluid connection with a fluid storage vessel. A filter cartridge is disposable within the filter housing, and a plunger pump is coupled at a second end of the filter housing. The plunger pump includes a housing having a rigid portion and a flexible portion. The flexible portion has a plunger-engaging portion for coupling to the plunger of an actuator. The flexible portion selectively movable with respect to the rigid portion via the actuator. Learn More

A U.S. Patent for a "Fluid Transport Apparatus, Flexible Conduit, Method of Manufacturing Flexible Conduit, and Storage Method" was awarded to Sartorius on October 13th, 2020. The present invention is a fluid transport apparatus defining an aseptic pathway configured to direct fluids therethrough is provided. The fluid transport apparatus may include a conduit extending between a first terminus and a second terminus. The conduit may include platinum catalyzed silicone rubber having methyl and phenyl side groups. A related flexible conduit, method of manufacturing a flexible conduit, and storage method are also provided. Learn More

A U.S. Patent for a "Single-Use Bioreactor" was awarded to Lonza on October 13th, 2020. The present invention may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium... Learn More

A U.S. Patent for "Method for Measuring a Plurality of Status Parameters of a Fluid Contained in a Container" was awarded to Sartorius on October 13th, 2020. The present invention is a method for measuring status parameters of a fluid contained in a container. The container is configured for single use and has a wall on which a sensor-carrier plate is fixed in a fluid-tight manner. The plate carries sensors that are in operative contact with the internal chamber of the container and connected for data exchange with an external control unit that receives and processes the measurement data from the sensors. The sensor plate also carries a temporarily inactive duplicate of at least one... Learn More

A U.S. Patent for "Remote Life Science Laboratories and Storage Facilities" was awarded to Microsoft on October 6th, 2020. The present invention is an automated life science laboratory or a storage facility for biological specimens may be located together with or in close proximity to a data center. The location of the data center, the automated life science laboratory, and the storage facility may be a location in which land and/or electricity are less expensive than locations where the biological specimens are collected. The automated life science laboratory may have a high-capacity data connection to the data center. The life science laboratory, storage facility... Learn More

A U.S. Patent for "Method and Unit for the Sterile Filling of a Final Basic Container with Content Intended for the Biopharmaceutical Field" was awarded to Sartorius on October 6th, 2020. The present invention is a method for the sterile filling of at least one final basic container, includes: providing at least one sterile chamber having at least one sterile entrance port and providing a source container containing the content, located outside the chamber; providing a communication element, a dispensing element and at least one filling member, to form when all are assembled, a sterile fluid filling and transfer line; inserting at least one final container into the chamber... Learn More

A U.S. Patent for "Dual Pumping Arrangement for a Hollow Fiber Filter" was awarded to Repligen on October 6th, 2020. The present invention is a fluid filtration assembly that includes a filter housing having first and second ends and a connector for fluid communication with a fluid storage vessel. A filter element is disposable within the filter housing, and first and second pumps are coupled at the first and second ends of the filter housing. A controller may coordinate the operation of the first and second pumps to induce alternating tangential flow of fluid between the filter housing and the first and second pumps. At least one of the first and second pumps... Learn More

A U.S. Patent for "Particle Separation and/or Purification of a Fluid" was awarded to Sartorius on October 6th, 2020.The present invention is a filter system with a first set of filter modules and a second set of filter modules that is different from the first set of filter modules. Each set of filter modules includes an inflow channel and an outflow channel. A fluid inlet is connected to the first set of filter modules, a fluid outlet is connected to the second set of filter modules, and a separation interface separates the first and second sets of filter modules. The separation interface has a first interface channel. Learn More

A U.S. Patent for "Activated Carbon for the Removal of Leachables and/or Extractables" was awarded to MilliporeSigma on October 6th, 2020. The present invention relates to the purification of target molecules like recombinant and/or biotherapeutic proteins. Activated carbon can be used to remove leachables and/or extractables resulting from disposable equipment employed in the process. Learn More

A U.S. Patent for "Purification of Proteins" was awarded to MilliporeSigma on October 6th, 2020. The present invention is a selectively soluble polymer capable of binding to one or more constituents in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and is rendered insoluble and precipitates out of solution upon a change in the process conditions. While in its solubilized state, the polymer is capable of binding to a selected entity within the stream such as impurities... Learn More

A U.S. Patent for "Continuous Multistep Process for Purifying Antibodies" was awarded to Sanofi on October 6th, 2020. The present invention is a method for purifying a protein from solution comprising: (a) a first chromatography step comprising: passing said solution over a first chromatography matrix, wherein the first chromatography matrix is a Protein A affinity chromatography matrix; eluting a crude protein eluent from the first chromatography matrix using a first elution buffer; (b) a second chromatography step comprising: passing the crude protein eluent obtained at the end of step (a) over a second chromatography matrix; eluting a protein eluate. Learn More

A U.S. Patent for "Delamination Resistant Glass Containers with Heat Tolerant Coatings" was awarded to Corning on September 29th, 2020. The present invention is delamination resistant glass pharmaceutical containers which may include an aluminosilicate glass having a Class HGA 1 hydrolytic resistance when tested according to ISO 720-1985 testing standard. The glass containers may also have a compressive stress layer with a depth of layer of greater than 25 .mu.m. A surface compressive stress of the glass containers may be greater than or equal to 350 MPa. The delamination resistant glass pharmaceutical containers may be ion exchange strengthened... Learn More

A U.S. Patent for "Use of a Device and a Method for Preparing Mixtures of Pharmaceutical Substances" was awarded to Sartorius on September 29th, 2020. The present invention is a method for preparing mixtures of pharmaceutical or biopharmaceutical substances, includes arranging a preparation container already made up with ingredients in a receptacle of a device, feeding a fluid from a storage container into the preparation container via a feed line, preparing the ingredients with the fluid into a substance mixture, and removing the substance mixture from the preparation container into a receiving container via a discharge line... Learn More

A U.S. Patent for "Depth Filters for Disposable Biotechnological Processes" was awarded to Millipore Sigma on September 29th, 2020. The present invention is a process for the primary clarification of feeds, including chemically treated flocculated feeds, containing the target biomolecules of interest such as mAbs, mammalian cell cultures, or bacterial cell cultures, using a primary clarification depth filtration device without the use of a primary clarification centrifugation step or a primary clarification tangential flow microfiltration step. The primary clarification depth filtration device contains a porous depth filter having graded porous layers... Learn More

A U.S. Patent for "Energy Recovery in a Freeze-Drying System" was awarded to SP Industries on September 22nd, 2020. The present invention is a freeze-drying system comprising: a freeze dryer chamber having one or more shelves disposed therein; a refrigerant condenser adapted to condense a hot gas refrigerant to a warm liquid refrigerant; a heat exchanger; a first fluid line from the heat exchanger to the one or more shelves; and a second fluid line, separate from the first fluid line, from the heat exchanger to the refrigerant condenser. Learn More

A U.S. Patent for a "Mixing Apparatus and Single-Use Apparatus for a Mixing Apparatus" was awarded to Levitronix on September 22nd, 2020. The present invention is a mixing apparatus for mixing substances includes a single-use apparatus and a reusable apparatus. The single-use apparatus includes a flexible mixing tank configured to receive the at least two substances to be mixed, is plastic, and has a rotor arranged in the mixing tank. The rotor includes an impeller configured to mix the at least two substances, the impeller being magnetically contactlessly drivable and free of coils and permanent magnets. Learn More

A U.S. Patent for "System and Method for Detachment of Cells in Fixed Bed Reactors" was awarded to Pall on September 22nd, 2020. The present invention is an apparatus for processing cells, comprising: a fixed bed reactor for the cells, the fixed bed reactor including a portion movable from a first position corresponding to a packed condition of a fixed bed to a second position corresponding to a depacked condition of the fixed bed, wherein the movable portion comprises a partition positioned within a compartment of the reactor including the fixed bed; an expandable retainer for retaining the partition in the first position... Learn More

A U.S. Patent for "Acoustic Transducer Driver and Controller" was awarded to FloDesign Sonics on September 22nd, 2020. The present invention is an acoustophoretic system is controlled and driven to attain a desired level of performance. An RF controller and a driver provide a frequency and power to an acoustic transducer, which can be implemented as a piezoelectric element, which presents a reactive load or a complex load. A controller implements a control technique for efficient transducer operation. The control technique can locate a frequency for operation that is at a reactance minimum or maximum for the system... Learn More

A U.S. Patent for "Methods of Manufacturing Therapeutic Proteins" was awarded to Momenta on September 15th, 2020. The present invention is a method of manufacturing a therapeutic protein, comprising: transfecting a mammalian cell which is cholesterol-auxotrophic and glutamine-auxotrophic with (i) a nucleic acid encoding a 3-ketosteroid reductase; (ii) a nucleic acid encoding a glutamine synthetase; (iii) a nucleic acid comprising an aminoglycoside antibiotic resistance gene; and (iv) a nucleic acid encoding the therapeutic protein. Learn More

A U.S. Patent for "Methods, Systems, and Apparatuses for Acoustically Separating and Washing Particles" was awarded to Sonosep on September 15th, 2020. The present invention is a method of washing particles in an acoustic chamber, the method comprising: a) flowing a suspension of particles in a suspension medium through a standing wave generated in a standing wave volume of the acoustic chamber to accumulate within the acoustic chamber at least some of the particles as raw particle concentrate; and b) flowing a wash medium through the acoustic chamber to wash within the acoustic chamber at least some of the particles. Learn More

A U.S. Patent for "Vessel Closures and Methods for Using and Manufacturing Same" was awarded to Sartorius on September 15th, 2020. The present invention is a fluid transfer hub, comprising: a first vessel closure; a second vessel closure; a gasket arranged between the first vessel closure and the second vessel closure; and a clamp at least partially surrounding the first vessel closure, the second vessel closure and the gasket, wherein the first vessel closure comprises: a first body, one or more apertures extending axially through the first body, one or more first inserts extending axially through the one or more apertures, and a cast seal disposed within... Learn More

A U.S. Patent for a "Method of Actuation of an Alternating Tangential Flow Diaphragm Pump" was awarded to Repligen on September 15th, 2020. The present invention is a method of controlling a diaphragm pump, comprising: actuating a pumping operation of a fluid from a first container by alternating a supply of a positive air flow and a negative air flow to an air side of a diaphragm pump through an air flow line connected to an output of an air flow regulator; suctioning the fluid from the first container by applying the supply of negative air flow to the air side of the diaphragm pump; directly monitoring, by a plurality of sensors along a permeate fluid pathway. Learn More

A U.S. Patent for "Temperature Controlled Support Surfaces for Single Use Flexible Wall Systems" was awarded to GE on September 15th, 2020. The present invention is a jacketed, tiered baffle, bioreactor tank, comprising: an outer cylindrical-shaped jacket; and a cylindrical tank having an inner tank surface defining a chamber configured for supporting a flexible bag disposed within the chamber, and an outer tank surface having tiered baffles configured for routing a heat exchange fluid around the entirety of the outer tank surface, the cylindrical tank disposed axially within the outer cylindrical-shaped jacket, the cylindrical tank having at least one. Learn More

A U.S. Patent for a "System and Method for Production of On-Demand Proteins in a Portable Unit for Point of Care Delivery" was awarded to the University of Maryland on September 15th, 2020. The present invention is a cell-free bioprocessing system for the production of on-demand synthesized desired target protein, the system comprising: a production module for producing a protein; and a purification module for receiving the protein from the production module and for purifying the protein from reagents, wherein the production module comprises a bioreactor for cell-free based protein expression, wherein the bioreactor comprises a resin column... Learn More

A U.S. Patent for a "System and Method of Performing a Biological Experiment with Adaptive Cybernetic Control of Procedural Conditions" was awarded to Arctoris on September 15th, 2020. The present invention is a system for performing a biological experiment, the system comprising: a computer-readable storage medium including an experiment protocol and corrective protocols, the experiment protocol specifying a sequence of operations and prescribed conditions for carrying out the biological experiment, the corrective protocols specifying operations for remediating deviations of measurements of the prescribed conditions... Learn More

A U.S. Patent for "Device for a Biological Liquid Treatment Installation" was awarded to MilliporeSigma on September 8th, 2020. The present invention is a device for a biological liquid treatment installation, comprising: a base having a front face; a movable door movable between an open and a closed position, said movable door having a perimeter; a circuit comprising a plurality of connectors and a network having conduits for conveying liquid between said connectors, which circuit comprises a bag comprising two flexible films and said connectors, which circuit further comprises a press comprising a first shell disposed upright on said front face of said base. Learn More

A U.S. Patent for "Temperature Controlling Surfaces and Support Structures" was awarded to GE on September 8th, 2020. The present invention is a heat exchange module for use in a chemical, pharmaceutical or biological reactor system, comprising: a generally hollow body having an interior space for receiving a replaceable or single use reactant container, and at least one thermally conductive surface adapted to contact the replaceable or single use reactant container to facilitate heat transfer; a conduit forming a fluid circulation path within the body between an outer wall of the body and an inner wall of the body through which a heat exchange fluid can be circulated. Learn More

A U.S. Patent for "Tangential Flow Depth Filtration Systems and Methods of Filtration Using the Same" was awarded to Repligen on September 8th, 2020. The present invention is a bioprocessing system comprising: a process vessel; a tangential flow depth filtration (TFDF) unit comprising a thick-walled hollow fiber formed from at least one polymer and comprising a porous wall defining a lumen that is in fluid communication with the process vessel; a permeate fluid outlet in fluid communication with an exterior surface of the porous wall; and a pump in fluid communication with the lumen; wherein a density of the hollow fiber is between 51% and 56% of the density. Learn More

A U.S. Patent for a "Mechanical Bioreactor" was awarded to the University of Glasgow on September 8th, 2020. The present invention is a mechanical bioreactor for transferring vibrations to cell cultures for the purpose of stimulating various biological processes across a relatively large (relative to the amplitude of the vibrations) sample receiving surface. This bioreactor allows multiple samples to receive identical stimulation, which may enable the process to be significantly scaled up. The invention may be particularly applicable to nanoscale vibration amplitudes, where it is non-trivial to design a device that performs this function... Learn More

A U.S. Patent for "Methods for Modulating Production Profiles of Recombinant Proteins" was awarded to Ares Trading on September 8th, 2020. The present invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of a triazine dye or supplemented with an effective amount of a triazine dye, whereby production of said protein is increased relative to cells grown without said triazine dye or any other inducer. Learn More

A U.S. Patent for a "Method and Apparatus for Quantifying Solutions Comprised of Multiple Analytes" was awarded to Thermo Fisher on September 8th, 2020. The present invention is an analyzer system for determining the identity and concentration of at least one target analyte present in a gaseous or liquid sample utilizing the Raman optical scattering effect, said analyzer system comprising: (i) a laser light source emitting light having a wavelength in the ultraviolet to near infrared spectral region which generates Raman Stokes and anti-Stokes emissions when incident on said target analyte; (ii) a hollow core photonic band-gap (HCPBG) fiber optically connected... Learn More

A U.S. Patent for a "Laterally-Fed Membrane Chromatography Device" was awarded to McMaster University on September 1st, 2020. The present invention is a laterally-fed membrane chromatography device for removing a solute from a fluid is provided. The device has a top plate, a middle plate and a bottom plate. The top plate has an inlet and a top channel for directing the fluid from the inlet towards a membrane stack. The middle plate houses the membrane stack. The membrane stack has a leading edge for receiving the fluid from the top channel, a trailing edge for distributing the fluid to the bottom channel, and is configured to remove... Learn More

A U.S. Patent for a "Cell Culture Bag with Internal Dialysis Membrane" was awarded to GE on September 1st, 2020. The present invention is a flexible bag for cell cultivation, comprising: a) one or more walls; b) a cultivation compartment defined by the one or more walls; c) at least one dialysis compartment surrounded by the cultivation compartment, the at least one dialysis compartment being delimited from the cultivation compartment by at least one tubular dialysis membrane, wherein the at least one tubular dialysis membrane has a lumen, and two ends; and d) a first and second port, each port having one end extending... Learn More

A U.S. Patent for "Method of Manufacturing or Differentiating Mammalian Pluripotent Stem Cells or Progenitor Cells Using a Hollow Fiber Bioreactor" was awarded to StemoniX on September 1st, 2020. The present invention provides a method of manufacturing and differentiating mammalian stem cells, and in one embodiment human induced pluripotent stem cells (iPSc), e.g., manufacturing neuron progenitors, e.g., derived from iPSC, on a large scale by the use of an automated hollow fiber reactor system. In one embodiment, human iPSc that can be differentiated into cardiomyocytes or neuron progenitors are provided. Learn More

A U.S. Patent for "Ultrafiltration Membranes and Methods of Making" was awarded to MilliporeSigma on August 25th, 2020. The present invention is a multilayered, flat sheet, composite membrane produced from at least two polymer solutions, the flat sheet membrane having at least one asymmetric ultrafiltration layer in which particles having a 20 to 100 nanometer (nm) diameter are retained and at least one asymmetric microporous layer in which particles larger than 0.1 .mu.m are retained, wherein the polymer solutions include a first polymer solution that forms the at least one ultrafiltration layer and a second polymer solution that forms the at least one microporous layer... Learn More

A U.S. Patent for "Multi-Analyte Sensing Using Hydrogels and Magnetometers" was awarded to Applied Biosensors on August 25th, 2020. The present invention is a sensor, comprising: a sensor probe having at least one sensor unit associated therewith, each sensor unit comprising: a hydrogel; a permanent magnetic sheet coupled to one side of the hydrogel to form a laminate structure; and a magnetometer coupled to a side of the hydrogel opposite the permanent magnetic sheet such that swelling or deswelling of the hydrogel causes movement of the permanent magnetic sheet relative to the magnetometer, wherein an entirety... Learn More

A U.S. Patent for "Method for Producing a Recombinant Protein on a Manufacturing Scale" was awarded to Boehringer Ingelheim on August 25th, 2020. The present invention is a method for plasmid-free production of a heterologous protein of interest, comprising the steps of a) cultivating bacterial expression host cells in a culture medium, said cultivating comprising a mode that employs adding a feeding medium, wherein said mode is selected from fed-batch mode, semi-continuous mode, and continuous mode, wherein (i) one copy of a DNA construct is integrated in the chromosome of the host cells, and wherein said DNA construct consists... Learn More

A U.S. Patent for "Chromatography Systems with Mobile Phase Generators" was awarded to Perkin Elmer on August 25th, 2020. The present invention is a gas chromatography system comprising a housing comprising an injector and an internal gas generator inside the housing, wherein the injector is configured to fluidically couple to a gas chromatography column within the housing through a first internal fluid line, wherein the internal gas generator is configured to fluidically couple to the gas chromatography column through a second fluid line, wherein the internal gas generator is configured to generate hydrogen gas... Learn More

A U.S. Patent Application for "Capillary Electrophoresis Inkjet Dispensing" was published by LI-COR on August 20th, 2020. The present invention is a method for dispensing an analyte from a capillary electrophoresis tube, the method comprising: applying a voltage potential through a capillary electrophoresis tube, the capillary electrophoresis tube having a capillary outlet, and a capillary longitudinal axis proximate to the capillary outlet; impulsively pumping a sheath liquid through a microfluidic pump chamber having an internal region that is configured to be deformed by an impulsive pump element, wherein the capillary electrophoresis tube extends... Learn More

A U.S. Patent Application for an "Abrasion Resistant Film for Biocontainers" was published by MilliporeSigma on August 20th, 2020. The present invention is a material for biocontainers including a multilayered film, the multilayered film having an interior and exterior side, an abrasion-resistant substrate attached to the interior or the exterior side of the film wherein the substrate comprises a polymeric woven fibrous material or a polymeric non-woven fibrous material, a woven fibrous material or a non-woven fibrous material comprising metal fibers, a woven fibrous material or a non-woven fibrous material comprising glass fibers, or a woven fibrous material... Learn More

A U.S. Patent for a "Culture Method for Stable Proliferation of Pluripotent Stem Cell While Maintaining Undifferentiated State" was awarded to Ajinomoto on August 18th, 2020. The present invention is a feeder cell-free culture system comprising a medium, wherein the medium comprises: an albumin, at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a pharmaceutically acceptable salt thereof, based on a volume of the medium; and at least one sulfated saccharide or a pharmaceutically acceptable salt thereof dissolved in the medium and selected from the group consisting of dextran sulfate Na. Learn More

A U.S. Patent for a "Method of Producing a Polypeptide or Virus of Interest in a Continuous Cell Culture" was awarded to Baxalta on August 18th, 2020. The present invention described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system... Learn More

A U.S. Patent Application for an "Enhanced Stability Filter Integrity Test" was published by MilliporeSigma on August 13th, 2020. The present invention is a method of integrity testing a porous material is disclosed, providing a porous material suitable for filtration to be tested, said porous material having a first surface and a second surface; wetting said porous material with a wetting liquid; providing a gas stream comprising at least first and second gases humidified with said wetting liquid below the saturation vapor pressure of said wetting liquid and wherein said humidified gas stream has a humidity of 50-99%; introducing said gas stream... Learn More

A U.S. Patent Application for "Substantially Aseptic Assembly for Bioprocessing Fluids" was published by Sartorius on August 13th, 2020. The present invention is an assembly for processing fluids comprising: a vessel having an opening; a vessel closure sealingly engaged to the opening of the vessel, the vessel closure comprising one or more apertures extending through the vessel closure; one or more inserts extending through the one or more apertures; and a respiratory assembly engaged with the vessel closure, the respiratory assembly comprising: a housing; and one or more gas permeable membranes engaged with the housing. Learn More