Patents

A U.S. Patent for a "Process for Manufacturing Factor VIII Having an Improved Ratio of FVIII:C/FVIII/AG" was awarded to Octapharma on November 3rd, 2020. The present invention is a process for manufacturing of a Factor VIII product having a ratio of FVIII:C/FVIII:Ag of at least 0.7 in the Factor VIII product by using chromatography wherein at least one chromatographic step is performed by means of employing; An affinity chromatography resin having an affinity for specifically binding of Factor VIII which is effected by an affinity ligand which is immobilised on the affinity chromatography resin, said affinity ligand is a 13 kD yeast derived Fab antibody fragment directed to the Factor VIII molecule. An anionic chromatography resin. A size exclusion resin...
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A U.S. Patent for "Regulated Vacuum Off Gassing of Gas Filter for Fluid Processing System and Related Methods" was awarded to Thermo Fisher on November 3rd, 2020. The present invention is a method for filtering a gas, the method comprising: delivering a gas under a positive pressure into a compartment of a gas filter assembly; applying a partial vacuum to the gas filter assembly so that the partial vacuum assists in drawing the gas delivered into the compartment under the positive pressure through a porous filter body of the gas filter assembly that is at least partially disposed within the compartment of the gas filter assembly; regulating the application of the partial vacuum based on a pressure reading of the gas upstream of the gas filter assembly...
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A U.S. Patent for "Virus Purification" was awarded to Kentucky Bioprocessing on November 3rd, 2020. The present invention is a method for purifying at least one virus harvested from a source organism, comprising: removing cellular debris from the virus by passing the at least one virus through a tangential flow filtration apparatus comprising a porous ceramic filter; concentrating the virus; and performing separations comprising sub-steps of separating host cell contaminants from the virus, subjecting the virus to affinity chromatography to elute the virus, and subjecting the virus to multi-modal chromatography to separate residual impurities from the virus based on size differences between the impurities as retentate and the virus as permeate. 
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A U.S. Patent for "Pellets Used in Cell Culture and Methods of Making Thereof" was awarded to Thermo Fisher on November 3rd, 2020. The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to handle either before reconstitution...
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A U.S. Patent for "Multimodal Anion Exchange Matrices" was awarded to Cytiva on October 27th, 2020. The present invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R.sub.1-L.sub.1-N(R.sub.3)-L.sub.2-R, immobilized on a support, wherein R.sub.1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L.sub.1 is either a methylene group or a covalent bond; R.sub.2 is a five-or six-membered, substituted or non-substituted ring structure; L.sub.2 is either a methylene group or a covalent bond; R.sub.3 is a methyl group; and wherein if R.sub.1 is a hydroxyethyl group and L.sub.1 is a covalent bond, R.sub.2 is a substituted aromatic ring structure or a substituted or non-substituted...
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A U.S. Patent for a "Cell Line Screening Method" was awarded to Larix Bioscience on October 27th, 2020. The present invention provides a novel cell line development method useful to screen for recombinant protein production. The method utilizes a membrane-anchored reporter or an intracellular reporter residing in the expression vector for a gene of interest to facilitate initial cell selection by FACS or MACS. A switching mechanism can be used to delete the reporter from the chromosome by providing an appropriate DNA recombinase, which turns the selected cells into production cells that secrete the protein of interest without co-expression of the reporter.
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A U.S. Patent for "Devices, Systems and Methods for Zone Sterilization" was awarded to Burkert on October 27th, 2020. The present invention is a gas transfer system including a housing, a pressurized gas canister held by the housing, a first passageway in fluid communication with the pressurized gas canister and configured to supply pressurized pre-sterilization gas from the pressurized gas canister to a fluid flow component, a gas discharge canister held by the housing; and a second passageway in fluid communication with the gas discharge canister and configured to supply post soak gas from the fluid flow component to the gas discharge canister. A humidification system or device is included for the sterile humidification of sterilizing gas. 
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A U.S. Patent for a "Large Scale Acoustic Separation Device" was awarded to FloDesign Sonics on October 27th, 2020. The present invention is a method for separating a secondary fluid or particulate from a mixture with a host fluid, comprising: placing the mixture in an acoustophoretic device that comprises: an acoustic chamber; at least one ultrasonic transducer coupled to the acoustic chamber and configured to generate a multi-dimensional acoustic standing wave in the acoustic chamber; a reflector across the acoustic chamber from the at least one ultrasonic transducer; and an interior volume of at least 40 cubic inches; exciting the at least one transducer in a higher order mode to generate the multi-dimensional acoustic standing wave ... Learn More

A U.S. Patent for "Immunoglobulin-Binding Proteins and Their Use in Affinity Purification" was awarded to Navigo Proteins on October 20th, 2020. The present invention relates to non-natural binding proteins comprising one or more non-natural immunoglobulin (Ig) binding domains wherein at least one non-natural lg-binding domain comprises the amino acid sequence X1 X2X3XiXsX5X7 XsQQX11AFYX1sX15LX1 sX19PX21 LX23X24X2sQRX28X2gf IQSLKDDPSXio SXi2Xi3Xi4LXi5EAXigKLXs2Xs3Xs4QXs5PX. The disclosure also relates to compositions such as affinity matrices comprising the non-natural Ig-binding proteins of the invention. Learn More

A U.S. Patent for "Light Emitting Diode Photobioreactors and Methods of Use" was awarded to Avespa on October 20th, 2020. The present invention is a photobioreactor system and a process for its use, whereby water and nutrients from multiple sources are balanced (mixed using aeration) to the specific requirements of the particular photosynthetic organism strain used, sterilized, further mixed to balance the system and seeded with the photosynthetic microorganism, e.g. microalgae (dilution of a concentrated stock or added to an existing algal biomass). In accordance with such an embodiment, the algal biomass is then grown for a most efficient number of hours in a totally controlled environment... Learn More

A U.S. Patent for a “Mixing Vessel with Locking Assembly for Locking a Mixing Assembly in Storage Position and Mixing Impeller with Central Disc-Like Member” was awarded to Sartorius on October 20th, 2020. The present invention is a mixing vessel for accommodating components to be mixed has a container with at least one mounting depression in a side wall of the container. The mounting depression is adapted so that a mixing impeller housing of a mixing impeller is at least partly insertable, in which at least one magnet is housed for being magnetically connectable to a drive device to be driven. A locking assembly is attachable to the mounting depression from outside for locking the mixing impeller in a storage position… Learn More

A U.S. Patent for “Reactor Surface Finish Remediation” was awarded to Lonza on October 20th, 2020. The present invention is biopharmaceutical processing equipment, comprising: a vessel defining a volume for receiving a proteinaceous processing material, the vessel having an interior reactor surface that is configured to contact proteinaceous processing material when contained within the volume of the vessel, the biopharmaceutical processing equipment further comprising a clean-in-place (CIP) apparatus configured to clean the surface, and wherein the interior reactor surface is made from stainless steel and has a pre-commissioning surface roughness of greater than or equal to about 25 Ra Max. Learn More

A U.S. Patent for a "Method of Thermally Disinfecting a Centrifuge" was awarded to GEA on October 13th, 2020. The present invention is a method for thermally disinfecting a centrifuge by reducing a quantity of germs in the centrifuge, the method comprising: reducing the quantity of gems in the centrifuge using vapor that is not pressurized or which is at atmospheric pressure, wherein the centrifuge has a rotatable drum and is configured to centrifugally processing a product, wherein the vapor is passed through a feed, distributor ducts, a centrifuging chamber, a separating disk stack, and an outlet of the centrifuge, wherein, while the vapor is passed through the centrifuge, the drum is rotated. Learn More

A U.S. Patent for a "Chromatography Membranes, Devices Containing Them, and Methods of Use Thereof " was awarded to MilliporeSigma on October 13th, 2020. The present invention are fluid treatment devices for use in tangential flow filtration, comprising a housing unit and a composite material, wherein the composite material comprises: a support member comprising a plurality of pores extending through the support member; and a non-self-supporting macroporous cross-linked gel comprising macropores having an average size of 10 nm to 3000 nm, said macroporous gel being located in the pores of the support member. Learn More

A U.S. Patent for "Hydrophilic Filtration During the Manufacture of Vaccine Adjuvants" was awarded to Novartis on October 13th, 2020. The present invention is an improved method for the manufacture of an oil-in water emulsion involves three procedures: (i) preparation of a preliminary emulsion; (ii) micro fluidization of the preliminary emulsion to reduce its droplet size; and (iii) filtration, of the microfluidized emulsion through a hydrophilic membrane. The emulsions are useful as vaccine adjuvants.
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A U.S. Patent for a "Plunger Pumping Arrangement for a Hollow Fiber Filter" was awarded to Repligen on October 13th, 2020. The present invention is a fluid filtration assembly that includes a filter housing having a first end for fluid connection with a fluid storage vessel. A filter cartridge is disposable within the filter housing, and a plunger pump is coupled at a second end of the filter housing. The plunger pump includes a housing having a rigid portion and a flexible portion. The flexible portion has a plunger-engaging portion for coupling to the plunger of an actuator. The flexible portion selectively movable with respect to the rigid portion via the actuator. Learn More

A U.S. Patent for a "Fluid Transport Apparatus, Flexible Conduit, Method of Manufacturing Flexible Conduit, and Storage Method" was awarded to Sartorius on October 13th, 2020. The present invention is a fluid transport apparatus defining an aseptic pathway configured to direct fluids therethrough is provided. The fluid transport apparatus may include a conduit extending between a first terminus and a second terminus. The conduit may include platinum catalyzed silicone rubber having methyl and phenyl side groups. A related flexible conduit, method of manufacturing a flexible conduit, and storage method are also provided. Learn More

A U.S. Patent for a "Single-Use Bioreactor" was awarded to Lonza on October 13th, 2020. The present invention may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium... Learn More

A U.S. Patent for "Method for Measuring a Plurality of Status Parameters of a Fluid Contained in a Container" was awarded to Sartorius on October 13th, 2020. The present invention is a method for measuring status parameters of a fluid contained in a container. The container is configured for single use and has a wall on which a sensor-carrier plate is fixed in a fluid-tight manner. The plate carries sensors that are in operative contact with the internal chamber of the container and connected for data exchange with an external control unit that receives and processes the measurement data from the sensors. The sensor plate also carries a temporarily inactive duplicate of at least one... Learn More

A U.S. Patent for "Remote Life Science Laboratories and Storage Facilities" was awarded to Microsoft on October 6th, 2020. The present invention is an automated life science laboratory or a storage facility for biological specimens may be located together with or in close proximity to a data center. The location of the data center, the automated life science laboratory, and the storage facility may be a location in which land and/or electricity are less expensive than locations where the biological specimens are collected. The automated life science laboratory may have a high-capacity data connection to the data center. The life science laboratory, storage facility... Learn More

A U.S. Patent for "Method and Unit for the Sterile Filling of a Final Basic Container with Content Intended for the Biopharmaceutical Field" was awarded to Sartorius on October 6th, 2020. The present invention is a method for the sterile filling of at least one final basic container, includes: providing at least one sterile chamber having at least one sterile entrance port and providing a source container containing the content, located outside the chamber; providing a communication element, a dispensing element and at least one filling member, to form when all are assembled, a sterile fluid filling and transfer line; inserting at least one final container into the chamber... Learn More

A U.S. Patent for "Dual Pumping Arrangement for a Hollow Fiber Filter" was awarded to Repligen on October 6th, 2020. The present invention is a fluid filtration assembly that includes a filter housing having first and second ends and a connector for fluid communication with a fluid storage vessel. A filter element is disposable within the filter housing, and first and second pumps are coupled at the first and second ends of the filter housing. A controller may coordinate the operation of the first and second pumps to induce alternating tangential flow of fluid between the filter housing and the first and second pumps. At least one of the first and second pumps... Learn More

A U.S. Patent for "Particle Separation and/or Purification of a Fluid" was awarded to Sartorius on October 6th, 2020.The present invention is a filter system with a first set of filter modules and a second set of filter modules that is different from the first set of filter modules. Each set of filter modules includes an inflow channel and an outflow channel. A fluid inlet is connected to the first set of filter modules, a fluid outlet is connected to the second set of filter modules, and a separation interface separates the first and second sets of filter modules. The separation interface has a first interface channel. Learn More

A U.S. Patent for "Activated Carbon for the Removal of Leachables and/or Extractables" was awarded to MilliporeSigma on October 6th, 2020. The present invention relates to the purification of target molecules like recombinant and/or biotherapeutic proteins. Activated carbon can be used to remove leachables and/or extractables resulting from disposable equipment employed in the process. Learn More

A U.S. Patent for "Purification of Proteins" was awarded to MilliporeSigma on October 6th, 2020. The present invention is a selectively soluble polymer capable of binding to one or more constituents in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and is rendered insoluble and precipitates out of solution upon a change in the process conditions. While in its solubilized state, the polymer is capable of binding to a selected entity within the stream such as impurities... Learn More

A U.S. Patent for "Continuous Multistep Process for Purifying Antibodies" was awarded to Sanofi on October 6th, 2020. The present invention is a method for purifying a protein from solution comprising: (a) a first chromatography step comprising: passing said solution over a first chromatography matrix, wherein the first chromatography matrix is a Protein A affinity chromatography matrix; eluting a crude protein eluent from the first chromatography matrix using a first elution buffer; (b) a second chromatography step comprising: passing the crude protein eluent obtained at the end of step (a) over a second chromatography matrix; eluting a protein eluate. Learn More

A U.S. Patent for "Delamination Resistant Glass Containers with Heat Tolerant Coatings" was awarded to Corning on September 29th, 2020. The present invention is delamination resistant glass pharmaceutical containers which may include an aluminosilicate glass having a Class HGA 1 hydrolytic resistance when tested according to ISO 720-1985 testing standard. The glass containers may also have a compressive stress layer with a depth of layer of greater than 25 .mu.m. A surface compressive stress of the glass containers may be greater than or equal to 350 MPa. The delamination resistant glass pharmaceutical containers may be ion exchange strengthened... Learn More

A U.S. Patent for "Use of a Device and a Method for Preparing Mixtures of Pharmaceutical Substances" was awarded to Sartorius on September 29th, 2020. The present invention is a method for preparing mixtures of pharmaceutical or biopharmaceutical substances, includes arranging a preparation container already made up with ingredients in a receptacle of a device, feeding a fluid from a storage container into the preparation container via a feed line, preparing the ingredients with the fluid into a substance mixture, and removing the substance mixture from the preparation container into a receiving container via a discharge line... Learn More

A U.S. Patent for "Depth Filters for Disposable Biotechnological Processes" was awarded to Millipore Sigma on September 29th, 2020. The present invention is a process for the primary clarification of feeds, including chemically treated flocculated feeds, containing the target biomolecules of interest such as mAbs, mammalian cell cultures, or bacterial cell cultures, using a primary clarification depth filtration device without the use of a primary clarification centrifugation step or a primary clarification tangential flow microfiltration step. The primary clarification depth filtration device contains a porous depth filter having graded porous layers... Learn More

A U.S. Patent for "Energy Recovery in a Freeze-Drying System" was awarded to SP Industries on September 22nd, 2020. The present invention is a freeze-drying system comprising: a freeze dryer chamber having one or more shelves disposed therein; a refrigerant condenser adapted to condense a hot gas refrigerant to a warm liquid refrigerant; a heat exchanger; a first fluid line from the heat exchanger to the one or more shelves; and a second fluid line, separate from the first fluid line, from the heat exchanger to the refrigerant condenser. Learn More

A U.S. Patent for a "Mixing Apparatus and Single-Use Apparatus for a Mixing Apparatus" was awarded to Levitronix on September 22nd, 2020. The present invention is a mixing apparatus for mixing substances includes a single-use apparatus and a reusable apparatus. The single-use apparatus includes a flexible mixing tank configured to receive the at least two substances to be mixed, is plastic, and has a rotor arranged in the mixing tank. The rotor includes an impeller configured to mix the at least two substances, the impeller being magnetically contactlessly drivable and free of coils and permanent magnets. Learn More

A U.S. Patent for "System and Method for Detachment of Cells in Fixed Bed Reactors" was awarded to Pall on September 22nd, 2020. The present invention is an apparatus for processing cells, comprising: a fixed bed reactor for the cells, the fixed bed reactor including a portion movable from a first position corresponding to a packed condition of a fixed bed to a second position corresponding to a depacked condition of the fixed bed, wherein the movable portion comprises a partition positioned within a compartment of the reactor including the fixed bed; an expandable retainer for retaining the partition in the first position... Learn More

A U.S. Patent for "Acoustic Transducer Driver and Controller" was awarded to FloDesign Sonics on September 22nd, 2020. The present invention is an acoustophoretic system is controlled and driven to attain a desired level of performance. An RF controller and a driver provide a frequency and power to an acoustic transducer, which can be implemented as a piezoelectric element, which presents a reactive load or a complex load. A controller implements a control technique for efficient transducer operation. The control technique can locate a frequency for operation that is at a reactance minimum or maximum for the system... Learn More

A U.S. Patent for "Methods of Manufacturing Therapeutic Proteins" was awarded to Momenta on September 15th, 2020. The present invention is a method of manufacturing a therapeutic protein, comprising: transfecting a mammalian cell which is cholesterol-auxotrophic and glutamine-auxotrophic with (i) a nucleic acid encoding a 3-ketosteroid reductase; (ii) a nucleic acid encoding a glutamine synthetase; (iii) a nucleic acid comprising an aminoglycoside antibiotic resistance gene; and (iv) a nucleic acid encoding the therapeutic protein. Learn More

A U.S. Patent for "Methods, Systems, and Apparatuses for Acoustically Separating and Washing Particles" was awarded to Sonosep on September 15th, 2020. The present invention is a method of washing particles in an acoustic chamber, the method comprising: a) flowing a suspension of particles in a suspension medium through a standing wave generated in a standing wave volume of the acoustic chamber to accumulate within the acoustic chamber at least some of the particles as raw particle concentrate; and b) flowing a wash medium through the acoustic chamber to wash within the acoustic chamber at least some of the particles. Learn More

A U.S. Patent for "Vessel Closures and Methods for Using and Manufacturing Same" was awarded to Sartorius on September 15th, 2020. The present invention is a fluid transfer hub, comprising: a first vessel closure; a second vessel closure; a gasket arranged between the first vessel closure and the second vessel closure; and a clamp at least partially surrounding the first vessel closure, the second vessel closure and the gasket, wherein the first vessel closure comprises: a first body, one or more apertures extending axially through the first body, one or more first inserts extending axially through the one or more apertures, and a cast seal disposed within... Learn More

A U.S. Patent for a "Method of Actuation of an Alternating Tangential Flow Diaphragm Pump" was awarded to Repligen on September 15th, 2020. The present invention is a method of controlling a diaphragm pump, comprising: actuating a pumping operation of a fluid from a first container by alternating a supply of a positive air flow and a negative air flow to an air side of a diaphragm pump through an air flow line connected to an output of an air flow regulator; suctioning the fluid from the first container by applying the supply of negative air flow to the air side of the diaphragm pump; directly monitoring, by a plurality of sensors along a permeate fluid pathway. Learn More

A U.S. Patent for "Temperature Controlled Support Surfaces for Single Use Flexible Wall Systems" was awarded to GE on September 15th, 2020. The present invention is a jacketed, tiered baffle, bioreactor tank, comprising: an outer cylindrical-shaped jacket; and a cylindrical tank having an inner tank surface defining a chamber configured for supporting a flexible bag disposed within the chamber, and an outer tank surface having tiered baffles configured for routing a heat exchange fluid around the entirety of the outer tank surface, the cylindrical tank disposed axially within the outer cylindrical-shaped jacket, the cylindrical tank having at least one. Learn More

A U.S. Patent for a "System and Method for Production of On-Demand Proteins in a Portable Unit for Point of Care Delivery" was awarded to the University of Maryland on September 15th, 2020. The present invention is a cell-free bioprocessing system for the production of on-demand synthesized desired target protein, the system comprising: a production module for producing a protein; and a purification module for receiving the protein from the production module and for purifying the protein from reagents, wherein the production module comprises a bioreactor for cell-free based protein expression, wherein the bioreactor comprises a resin column... Learn More

A U.S. Patent for a "System and Method of Performing a Biological Experiment with Adaptive Cybernetic Control of Procedural Conditions" was awarded to Arctoris on September 15th, 2020. The present invention is a system for performing a biological experiment, the system comprising: a computer-readable storage medium including an experiment protocol and corrective protocols, the experiment protocol specifying a sequence of operations and prescribed conditions for carrying out the biological experiment, the corrective protocols specifying operations for remediating deviations of measurements of the prescribed conditions... Learn More

A U.S. Patent for "Device for a Biological Liquid Treatment Installation" was awarded to MilliporeSigma on September 8th, 2020. The present invention is a device for a biological liquid treatment installation, comprising: a base having a front face; a movable door movable between an open and a closed position, said movable door having a perimeter; a circuit comprising a plurality of connectors and a network having conduits for conveying liquid between said connectors, which circuit comprises a bag comprising two flexible films and said connectors, which circuit further comprises a press comprising a first shell disposed upright on said front face of said base. Learn More

A U.S. Patent for "Temperature Controlling Surfaces and Support Structures" was awarded to GE on September 8th, 2020. The present invention is a heat exchange module for use in a chemical, pharmaceutical or biological reactor system, comprising: a generally hollow body having an interior space for receiving a replaceable or single use reactant container, and at least one thermally conductive surface adapted to contact the replaceable or single use reactant container to facilitate heat transfer; a conduit forming a fluid circulation path within the body between an outer wall of the body and an inner wall of the body through which a heat exchange fluid can be circulated. Learn More

A U.S. Patent for "Tangential Flow Depth Filtration Systems and Methods of Filtration Using the Same" was awarded to Repligen on September 8th, 2020. The present invention is a bioprocessing system comprising: a process vessel; a tangential flow depth filtration (TFDF) unit comprising a thick-walled hollow fiber formed from at least one polymer and comprising a porous wall defining a lumen that is in fluid communication with the process vessel; a permeate fluid outlet in fluid communication with an exterior surface of the porous wall; and a pump in fluid communication with the lumen; wherein a density of the hollow fiber is between 51% and 56% of the density. Learn More

A U.S. Patent for a "Mechanical Bioreactor" was awarded to the University of Glasgow on September 8th, 2020. The present invention is a mechanical bioreactor for transferring vibrations to cell cultures for the purpose of stimulating various biological processes across a relatively large (relative to the amplitude of the vibrations) sample receiving surface. This bioreactor allows multiple samples to receive identical stimulation, which may enable the process to be significantly scaled up. The invention may be particularly applicable to nanoscale vibration amplitudes, where it is non-trivial to design a device that performs this function... Learn More

A U.S. Patent for "Methods for Modulating Production Profiles of Recombinant Proteins" was awarded to Ares Trading on September 8th, 2020. The present invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of a triazine dye or supplemented with an effective amount of a triazine dye, whereby production of said protein is increased relative to cells grown without said triazine dye or any other inducer. Learn More

A U.S. Patent for a "Method and Apparatus for Quantifying Solutions Comprised of Multiple Analytes" was awarded to Thermo Fisher on September 8th, 2020. The present invention is an analyzer system for determining the identity and concentration of at least one target analyte present in a gaseous or liquid sample utilizing the Raman optical scattering effect, said analyzer system comprising: (i) a laser light source emitting light having a wavelength in the ultraviolet to near infrared spectral region which generates Raman Stokes and anti-Stokes emissions when incident on said target analyte; (ii) a hollow core photonic band-gap (HCPBG) fiber optically connected... Learn More

A U.S. Patent for a "Laterally-Fed Membrane Chromatography Device" was awarded to McMaster University on September 1st, 2020. The present invention is a laterally-fed membrane chromatography device for removing a solute from a fluid is provided. The device has a top plate, a middle plate and a bottom plate. The top plate has an inlet and a top channel for directing the fluid from the inlet towards a membrane stack. The middle plate houses the membrane stack. The membrane stack has a leading edge for receiving the fluid from the top channel, a trailing edge for distributing the fluid to the bottom channel, and is configured to remove... Learn More

A U.S. Patent for a "Cell Culture Bag with Internal Dialysis Membrane" was awarded to GE on September 1st, 2020. The present invention is a flexible bag for cell cultivation, comprising: a) one or more walls; b) a cultivation compartment defined by the one or more walls; c) at least one dialysis compartment surrounded by the cultivation compartment, the at least one dialysis compartment being delimited from the cultivation compartment by at least one tubular dialysis membrane, wherein the at least one tubular dialysis membrane has a lumen, and two ends; and d) a first and second port, each port having one end extending... Learn More

A U.S. Patent for "Method of Manufacturing or Differentiating Mammalian Pluripotent Stem Cells or Progenitor Cells Using a Hollow Fiber Bioreactor" was awarded to StemoniX on September 1st, 2020. The present invention provides a method of manufacturing and differentiating mammalian stem cells, and in one embodiment human induced pluripotent stem cells (iPSc), e.g., manufacturing neuron progenitors, e.g., derived from iPSC, on a large scale by the use of an automated hollow fiber reactor system. In one embodiment, human iPSc that can be differentiated into cardiomyocytes or neuron progenitors are provided. Learn More

A U.S. Patent for "Ultrafiltration Membranes and Methods of Making" was awarded to MilliporeSigma on August 25th, 2020. The present invention is a multilayered, flat sheet, composite membrane produced from at least two polymer solutions, the flat sheet membrane having at least one asymmetric ultrafiltration layer in which particles having a 20 to 100 nanometer (nm) diameter are retained and at least one asymmetric microporous layer in which particles larger than 0.1 .mu.m are retained, wherein the polymer solutions include a first polymer solution that forms the at least one ultrafiltration layer and a second polymer solution that forms the at least one microporous layer... Learn More

A U.S. Patent for "Multi-Analyte Sensing Using Hydrogels and Magnetometers" was awarded to Applied Biosensors on August 25th, 2020. The present invention is a sensor, comprising: a sensor probe having at least one sensor unit associated therewith, each sensor unit comprising: a hydrogel; a permanent magnetic sheet coupled to one side of the hydrogel to form a laminate structure; and a magnetometer coupled to a side of the hydrogel opposite the permanent magnetic sheet such that swelling or deswelling of the hydrogel causes movement of the permanent magnetic sheet relative to the magnetometer, wherein an entirety... Learn More

A U.S. Patent for "Method for Producing a Recombinant Protein on a Manufacturing Scale" was awarded to Boehringer Ingelheim on August 25th, 2020. The present invention is a method for plasmid-free production of a heterologous protein of interest, comprising the steps of a) cultivating bacterial expression host cells in a culture medium, said cultivating comprising a mode that employs adding a feeding medium, wherein said mode is selected from fed-batch mode, semi-continuous mode, and continuous mode, wherein (i) one copy of a DNA construct is integrated in the chromosome of the host cells, and wherein said DNA construct consists... Learn More

A U.S. Patent for "Chromatography Systems with Mobile Phase Generators" was awarded to Perkin Elmer on August 25th, 2020. The present invention is a gas chromatography system comprising a housing comprising an injector and an internal gas generator inside the housing, wherein the injector is configured to fluidically couple to a gas chromatography column within the housing through a first internal fluid line, wherein the internal gas generator is configured to fluidically couple to the gas chromatography column through a second fluid line, wherein the internal gas generator is configured to generate hydrogen gas... Learn More

A U.S. Patent Application for "Capillary Electrophoresis Inkjet Dispensing" was published by LI-COR on August 20th, 2020. The present invention is a method for dispensing an analyte from a capillary electrophoresis tube, the method comprising: applying a voltage potential through a capillary electrophoresis tube, the capillary electrophoresis tube having a capillary outlet, and a capillary longitudinal axis proximate to the capillary outlet; impulsively pumping a sheath liquid through a microfluidic pump chamber having an internal region that is configured to be deformed by an impulsive pump element, wherein the capillary electrophoresis tube extends... Learn More

A U.S. Patent Application for an "Abrasion Resistant Film for Biocontainers" was published by MilliporeSigma on August 20th, 2020. The present invention is a material for biocontainers including a multilayered film, the multilayered film having an interior and exterior side, an abrasion-resistant substrate attached to the interior or the exterior side of the film wherein the substrate comprises a polymeric woven fibrous material or a polymeric non-woven fibrous material, a woven fibrous material or a non-woven fibrous material comprising metal fibers, a woven fibrous material or a non-woven fibrous material comprising glass fibers, or a woven fibrous material... Learn More

A U.S. Patent for a "Culture Method for Stable Proliferation of Pluripotent Stem Cell While Maintaining Undifferentiated State" was awarded to Ajinomoto on August 18th, 2020. The present invention is a feeder cell-free culture system comprising a medium, wherein the medium comprises: an albumin, at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a pharmaceutically acceptable salt thereof, based on a volume of the medium; and at least one sulfated saccharide or a pharmaceutically acceptable salt thereof dissolved in the medium and selected from the group consisting of dextran sulfate Na. Learn More

A U.S. Patent for a "Method of Producing a Polypeptide or Virus of Interest in a Continuous Cell Culture" was awarded to Baxalta on August 18th, 2020. The present invention described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system... Learn More

A U.S. Patent Application for an "Enhanced Stability Filter Integrity Test" was published by MilliporeSigma on August 13th, 2020. The present invention is a method of integrity testing a porous material is disclosed, providing a porous material suitable for filtration to be tested, said porous material having a first surface and a second surface; wetting said porous material with a wetting liquid; providing a gas stream comprising at least first and second gases humidified with said wetting liquid below the saturation vapor pressure of said wetting liquid and wherein said humidified gas stream has a humidity of 50-99%; introducing said gas stream... Learn More

A U.S. Patent Application for "Substantially Aseptic Assembly for Bioprocessing Fluids" was published by Sartorius on August 13th, 2020. The present invention is an assembly for processing fluids comprising: a vessel having an opening; a vessel closure sealingly engaged to the opening of the vessel, the vessel closure comprising one or more apertures extending through the vessel closure; one or more inserts extending through the one or more apertures; and a respiratory assembly engaged with the vessel closure, the respiratory assembly comprising: a housing; and one or more gas permeable membranes engaged with the housing. Learn More

A U.S. Patent Application for "Stirred Tank Reactor Systems and Methods of Use" was published by Thermo Fisher on August 13th, 2020. The present invention is a reactor system that includes a support housing having an interior surface bounding a chamber, the chamber having a vertically extending central longitudinal axis. A flexible bag is disposed within the chamber of the support housing and has an interior surface bounding a compartment. A mixing element is disposed within the compartment of the flexible bag. A drive element, such as a drive shaft, is secured the mixing element, wherein the mixing element is laterally offset from and/or is angled... Learn More

A U.S. Patent Application for "Packed Bed Bioreactor Systems and Methods of Using the Same" was published by Corning on August 13th, 2020. The present invention is a cell culture matrix that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. Learn More

A U.S. Patent Application for "Systems and Methods for a Collapsible Chamber with Foldable Mixing Element" was published by Thermo Fisher on August 13th, 2020. The present invention is a system, method, and apparatus for culturing cells within a cell culture vessel having a mixing element. The cell culture system includes a flexible portion and a mixing element disposed therein. The mixing element includes a suspended foldable portion. The system is configured to reduce shear stress on cells without compromising mixing efficiency. This reduction is accomplished by using a mixing element having a large surface area allowing for reduced rotational speeds. Learn More

A U.S. Patent Application for "Online Biomass Capacitance Monitoring During Large Scale Production of Polypeptides of Interest" was published by Bristol Myers Squibb on August 13th, 2020. The present invention relates to the use of online biomass capacitance monitoring in perfusion cultures as a way to minimize volumes of cell culture media in large-scale manufacturing. In certain embodiments, a biomass capacitance probe is used to measure the viable cell density, and the viable cell density is used in conjunction with a pre-determined constant cell-specific perfusion rate to identify a target perfusion rate. Learn More

A U.S. Patent Application for "Illuminated Container for Growth of Biological Entities" was published by Corning on August 13th, 2020. The present invention is an illuminated container for the growth of biological entities. The container is illuminated by a flexible light diffusing fiber. The light diffusing fiber includes a core formed from a silica-based glass and a cladding in direct contact with the core. The light diffusing fiber also includes an outer polymer coating layer surrounding the cladding, the outer polymer coating layer being the cured product of a liquid polymer blend including a scattering material and a luminophore. Learn More

A U.S. Patent Application for "Sensing Systems" was published by Georgia Tech on August 13th, 2020. The present invention is a thin, soft sensor array system that can be deployed over the surfaces of bag bioreactors. The sensor array is fabricated using microfabrication processes along with functionalization methods necessary for measuring pH, glucose, and temperature. Miniature integrated circuit (IC) components are incorporated with the thin-film circuits, allowing for the real-time, on-board data analysis and wireless data communication. Learn More

A U.S. Patent Application for "Buffer Formulation Method and System" was published by Lonzaon August 13th, 2020. The present invention is a process for formulating buffer products for feeding to a bioprocess comprising: combining multiple buffer solutions to form a final buffer product, the multiple buffer solutions including at least a first buffer solution and a second buffer solution; measuring a conductivity of the final buffer product; measuring a refractive index of the final buffer product; comparing the measured conductivity to a preset conductivity range and comparing the measured refractive index to a preset refractive index range... Learn More

A U.S. Patent Application for "Method for Reducing the Trisulfide Level in Proteins" was published by Merck on August 13th, 2020. The present invention is a method for reducing the formation of trisulfide bonds in proteins comprising culturing cells expressing said proteins whereby a feed medium is added to the cell culture one or more times during cell culture that does not contain any significant amounts of cysteine or cystine but comprises S-sulfocysteine and/or salts thereof, whereby trisulfide linkage formation in said proteins is reduced relative to proteins expressed by cells cultured in a cell culture medium where cysteine and/or cystine... Learn More

A U.S. Patent Application for "Methods of Perfusion Culturing Using a Shake Flask and Microcarriers" was published by Sanofi on August 13th, 2020. The present invention is a method of testing the efficacy of a first or second liquid culture medium, a raw ingredient or supplement present in a first or second liquid culture medium, or a source of an adherent mammalian cell for use in a method of producing a recombinant protein, the method comprising: providing a shake flask containing an adherent mammalian cell disposed in a first liquid culture medium, wherein the first liquid culture medium occupies about 20% to about 30% of the volume... Learn More

A U.S. Patent for "Use of Caprylic Acid Precipitation for Protein Purification" was awarded to Bristol Myers Squibb on August 11th, 2020. The present invention is a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, comprising: a) subjecting the mixture to a first chromatography step, wherein the first chromatography is a protein A affinity chromatography; b) recovering the protein of interest in an elution solution having a low pH between 2.5 and 4; c) adding caprylic acid to the elution solution and then adjusting the pH to at least 5.5 to form a contaminant precipitate... Learn More

A U.S. Patent for "Heating Assembly for a Bioreactor and an Associated Method Thereof" was awarded to GE on August 11th, 2020. The present invention is a method comprising: receiving a cell-culture media comprising a plurality of cells in a culture vessel of a bioreactor; heating the cell-culture media using a heating assembly coupled to the culture vessel, wherein the heating assembly comprises: a holder comprising a plurality of segments coupled to each other to define a unitary structure, wherein the unitary structure comprises: a top end; a bottom end; and a side wall extending between the top end and the bottom end and along a circumferential... Learn More

A U.S. Patent for "Laboratory Instrument, System and Method for Instrument-Controlled Treatment of at Least One Laboratory Sample Using at Least One Consumable" was awarded to Eppendorf on August 11th, 2020. The present invention is a laboratory instrument for the instrument-controlled treatment of at least one fluid laboratory sample, comprising: at least one treatment apparatus, which is configured to perform the treatment of the at least one fluid laboratory sample by way of a pipetting process using an amount of at least one consumable being a container manufactured from plastic or comprising plastic, and a control apparatus... Learn More

A U.S. Patent Application for "Compositions and Methods for Detecting a Biological Contaminant" was published by Regeneron on August 6th, 2020. The present invention are compositions and methods useful to the determination of whether a microbial contaminant is present in a biological therapeutic production process. Specifically, an artificial positive amplification control plasmid and unique quantitative PCR detection probe are provided, which enables the rapid and real-time detection of a false positive result. Learn More

A U.S. Patent Application for "Systems and Methods for Using Behavior Data of Impurities and Target Proteins to Design Downstream Processes" was awarded to MIT on August 6th, 2020. The present invention are systems and methods for generating and evaluating candidate sequences of partitioning steps to partition at least one biologically produced product from at least one impurity. In some embodiments, a plurality of candidate sequences of partitioning steps may be generated, wherein at least one candidate sequence of the plurality of candidate sequences comprises a plurality of partitioning steps in a specified order. The plurality of candidate sequences may be evaluated. Learn More

A U.S. Patent for "Coated Cell Culture Apparatus and Methods of Use" was awarded to Worcester Polytechnic Institute on August 4th, 2020. The present invention is a device, and methods of using or making the device, for engineering cells in vitro are disclosed. In some aspects, a cell culture device comprises at least one glass or polymer surface configured for incubating cells in a culture medium; a charged molecule electrostatically bound to the surface; and a polyelectrolyte multilayer (PEM) electrostatically bound to the charged molecule, the PEM comprising one or more bi-layers of oppositely charged polyelectrolytes, and the PEM having a sufficient thickness... Learn More

A U.S. Patent for "Protein Purification by Anion Exchange Chromatography" was awarded to Baxalta on July 28th, 2020. The present invention is a method for the purification of a divalent cation binding protein comprising the steps of: (a) loading a first anion exchange resin material with the divalent cation binding protein in a loading buffer in the absence of divalent cations or at a low concentration thereof; (b) washing the loaded anion exchange resin material with a washing buffer comprising between 175 mM NaCl and 190 mM NaCl in the absence of divalent cations; (c) eluting the divalent cation binding protein with an eluant comprising a counter-anion... Learn More

A U.S. Patent for "Removal of Microorganisms from Fluid Samples Using Nanofiber Filtration Media" was awarded to MilliporeSigma on July 28th, 2020. The present invention is a method for removing microorganisms from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention. Microorganisms such as bacteria, particularly B. Diminuta, are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a B. Diminuta LRV greater than about 9, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. Learn More

A U.S. Patent for "Microcarriers, Matrices and Scaffolds for Culturing Mammalian Cells and Methods of Manufacture"was awarded to Howard University on July 28th, 2020. The present invention are microcarriers, matrices and scaffolds for growing mammalian cells which include copolymer particles and matrices comprising of polysaccharide-polyamine copolymers. The copolymeric particles and matrices have a pore size of at least 50 microns and permit the mammalian cells to grow both on an exterior surface of the particles and matrices and within an interior of the particles and matrices... Learn More

A U.S. Patent for "Cell Culture Methods for Expressing ADAMTS Protein" was awarded to Baxalta on July 28th, 2020. The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein. Learn More

A U.S. Patent for "Method for Continuous Purification" was awarded to an Individual on July 21st, 2020. The present invention is a device, comprising: a) a cylindrical module comprising one or more mixing apparatus within the cylindrical module, b) a resin, an input port for loading the resin and a tubing in communication with the resin loading input port within the cylindrical module, c) one or more air bleed valve(s), wherein the valve extracts accumulated air within the module, d) one or more first port(s) for adding a sample load solution and one or more first exit port(s) for removing sample load waste solution essentially absent sample... Learn More

A U.S. Patent for "Method for Producing a Product (e.g. polypeptide) in a Continuous Cell Culture Fermentation Process" was awarded to CMC Biologics on July 21st, 2020. The present invention is a method for producing a product selected from a biopolymer expressed by a cell or microorganism, an intracellular or extracellular product produced by a cell or microorganism, a periplasmatic product produced by a cell or microorganism, in a first and a second bioreactor in a chemostat fermentation process, wherein said first bioreactor comprises: i) optionally a first outlet having a separation device allowing impurities with a size below the size of the product... Learn More

A U.S. Patent for "Miniaturized Fourier-Transform Raman Spectrometer Systems and Methods" was awarded to MIT on July 21st, 2020. The present invention is a system, comprising: a light source to emit a probe beam; a probe waveguide, in optical communication with the light source, to receive the probe beam and cause at least a portion of the probe beam to interact with a sample, the interaction between the probe beam and the sample generating a Raman signal; a filter, in optical communication with the sample, to transmit the Raman signal and block the probe beam; a Fourier transform spectrometer in optical communication with the filter... Learn More

A U.S. Patent Application for "Systems and Methods for Manufacturing Biologically Produced Products" was published by MIT on July 16th, 2020. The present invention is a biomanufacturing system, comprising: a perfusion bioreactor, wherein the perfusion bioreactor comprises: a reaction chamber configured to receive at least one feed stream comprising at least one cell culture medium; a suspension comprising the at least one cell culture medium and at least a first type of biological cells configured to express at least one biologically-produced product; at least one filter probe at least partially submerged in the suspension... Learn More

A U.S. Patent for "Protein Quantification by Near Infrared Spectral Imaging" was awarded to Temple University on July 14th, 2020. The present invention is a method of quantifying protein in an electrophoresis gel, comprising the steps of: loading a sample including at least one protein into a gel via electrophoresis to generate at least one protein band; acquiring an infrared spectral dataset from the at least one protein band using near infrared spectral imaging (NIRSI); generating an infrared hyperspectral image from the infrared spectral dataset for each of the at least one protein band, the infrared hyperspectral image comprising a plurality of pixels.. Learn More

A U.S. Patent for "Ionic Strength Mediated pH Gradient Ion Exchange Chromatography" was awarded to Genentech on July 14th, 2020. The present invention is a method for analyzing a composition comprising a polypeptide and one or more charge variants of the polypeptide, the method comprising a) binding the polypeptide and one of more charge variants of the polypeptide to an ion-exchange chromatography material using a loading buffer, wherein the loading buffer is at a first pH and comprises a first ionic strength; b) eluting the polypeptide and one or more charge variants of the polypeptide from the ion-exchange chromatography material using an elution... Learn More

A U.S. Patent for "Integrated Continuous Manufacturing of Therapeutic Protein Drug Substances" was awarded to Sanofi on July 14th, 2020. The present invention isa process for manufacturing a drug substance comprising a recombinant .alpha.-galactosidase-A, the process comprising: (a) culturing cells in a perfusion bioreactor comprising a liquid culture medium under conditions that allow the cells to secrete the recombinant .alpha.-galactosidase-A into the medium, wherein at least 90% cell-free volumes of the medium are continuously or periodically removed from the perfusion bioreactor and fed into a periodic counter current... Learn More

A U.S. Patent for "Methods of Shifting an Isoelectric Profile of a Protein Product and Uses Thereof" was published by  Alexion on July 14th, 2020. The present invention is Eculizumab comprising seven protein subpopulations with an isoelectric point between about 5.45 and about 6.55 produced by a method comprising: (a) fed batch culturing mammalian cells comprising a nucleic acid encoding eculizumab in a production bioreactor under conditions sufficient to produce eculizumab for a first period of time comprising a growth phase or a growth phase and a stationary phase of the mammalian cells or that comprises a growth phase of the mammalian cells... Learn More

A U.S. Patent Application for "Methods for Viral Inactivation and Other Adventitious Agents" was published by  Genentechon July 9th, 2020. The present invention is a method for inactivating virus or adventitious agents in cell culture media while the media maintains suitability for cell culture, said method comprising (a) subjecting the cell culture media to high temperature short time (HTST) treatment; and (b) adjusting one or more parameters selected from the group consisting of pH, calcium level and phosphate level. Learn More

A U.S. Patent for "Methods and Compositions for Inactivating Enveloped Viruses" was awarded to Biogen on July 7th, 2020. Aspects of the present invention provide therapeutic recombinant protein preparations and methods of preparing therapeutic recombinant protein, which methods include contacting recombinant protein preparations with zwitterionic detergent at or above critical micelle concentration of the detergent. Learn More

A U.S. Patent for "Cell Culture Medium" was awarded to La Jolla Biologics on July 7th, 2020. The present invention provided herein, inter alia, are compositions and methods for culturing mammalian cells. In certain aspects, the composition is a medium containing one or more of a lithium ion source, one or more fatty acids, and/or ethanol. Use of any of the cell culture media described herein to culture cells that have been genetically engineered to produce one or more recombinant polypeptides (for example, antibodies) can result in increased titers, a more favorable glycosylation profile, and/or modulated (e.g. decreased) amounts of high and low molecular weight... Learn More

A U.S. Patent for "Decreasing Lactate Level and Increasing Polypeptide Production by Down Regulating the Expression of Lactate Dehydrogenase and Pyruvate Dehydrogenase Kinase" was awarded to Genentech on July 7th, 2020. The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. The inventors have discovered that concomitant downregulation of a LDH and PDHKs via siRNAs in cultured cells expressing polypeptides (e.g., antibodies) decreased lactate level, lactate production rate, and osmolality, and increased specific polypeptide productivity... Learn More

A U.S. Patent for "Enzyme Based Methods of Separating Protein from Protein-Rich Material" was awarded to the University of Akron on June 30th, 2020. The present invention is a method of hydrolyzing carbohydrates in a protein-rich material comprising the steps of: providing, in a first vessel, a supply of a protein-rich material having carbohydrates therein, wherein the entirety of the protein-rich material to be hydrolyzed is provided during the step of providing, such that the method is devoid of a step of adding further protein-rich material; adding, after the entirety of the protein-rich material is in the first vessel, a first supply of enzyme broth... Learn More

A U.S. Patent for "Use of Alkaline Washes During Chromatography to Remove Impurities" was awarded to Bristol-Myers Squibb on June 30th, 2020. The present invention is a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, comprising: a) subjecting the mixture to a first chromatography matrix, wherein the protein of interest binds to the first chromatography matrix, and wherein the first chromatography is an affinity chromatography selected from a protein A affinity chromatography and a protein G affinity chromatography; Learn More

A U.S. Patent for "Modulation of Charge Variants in a Monoclonal Antibody" was awarded to Outlook Therapeutics on June 30th, 2020. The present invention is a process for removing acid charge variants from a monoclonal antibody, comprising (a) loading a mammalian cell-expressed monoclonal antibody preparation onto a Protein A support, and eluting the monoclonal antibody from the Protein A support, thereby producing a first eluate comprising the monoclonal antibody; (b) loading the first eluate from step (a) onto an anion exchange and hydrophobic interaction (AEX/HIC) chromatography support, and allowing the first eluate to flow. Learn More

A U.S. Patent Application for "Media for Microorganism Culture and Related Compositions and Methods" was published by MIT on June 25th, 2020. The present invention is media for cultivating cells, e.g., Pichia Pastoris cells, as well as cultures containing cells, methods for making and using the media, and kits comprising the media. The media is particularly useful in the context of therapeutic protein production. Learn More

A U.S. Patent Application for "Improved Methods of Cell Culture" was published by Polpharma Biologicson June 25th, 2020. The present invention is a method for reducing the amount of acidic species and/or increasing the amount of basic species in a composition comprising a recombinant protein or for increasing the size of mammalian cells expressing a recombinant protein or for increasing the specific production rate of mammalian cells expressing a recombinant protein to simplify subsequent clarification and purification of the recombinant protein, said method comprising culturing mammalian cells expressing said recombinant protein... Learn More

A U.S. Patent for "Culture Medium for Proliferating a Stem Cell, Which Contains Sulfated Compounds" was awarded to Ajinomoto on June 23rd, 2020. The present invention is a method for culturing a stem cell, comprising culturing the stem cell in a medium comprising: a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof, wherein said sulfated compound or a pharmaceutically acceptable salt thereof is present in said medium at a concentration that promotes the growth of said stem cell in the presence of FGF, and wherein said sulfated compound or pharmaceutically acceptable salt thereof... Learn More

A U.S. Patent for "Data Lineage in an Intelligent Data Integration System" was awarded to Monsanto on June 23rd, 2020. The present invention is a computer-executed method that includes storing in a data store data attributes, data objects, and a data analysis tool (DAT). Each data object has an attribute set and an identifier set. The method includes identifying each data object that has an attribute set comprising a data attribute matching each reference data attribute associated with the DAT, and selecting an identified data object as an input data object for the DAT. The DAT generates a new data object as a function of the input data object... Learn More

A U.S. Patent for "Affinity Chromatography Wash Buffer" was awarded to Cephalon/TEVA on June 23rd, 2020. The present invention is a wash buffer that when used during affinity chromatography purification of a protein of interest, such as an antibody, the wash buffer significantly reduces the level of host cell proteins from the preparation. Following affinity chromatography with the wash buffer, the protein of interest may be further purified using membrane chromatography. Learn More

A U.S. Patent for "Cryopreservation Bags and Method of Use Thereof for Closed System, High Capacity Cell-Banking" was awarded to Boehringer Ingelheim on June 23rd, 2020. The present invention is a high-capacity cryopreservation bag, which is adapted for use in a high capacity biological material, closed cryopreservation banking system, and which is resistant to liquid nitrogen temperatures comprising: a. a first fluorinated ethylene propylene (FEP) enclosure; b. at least two sterile-weldable fluid conduits adapted for use in a high capacity biological material, closed banking system; c. a first rigid FEP inlet conduit, in fluid connection... Learn More

A U.S. Patent for a "Method for Rapidly Screening Microbial Hosts to Identify Certain Strains with Improved Yield and/or Quality in the Expression of Heterologous Proteins" was awarded to Pfenex on June 23rd, 2020. The present invention is a method for selecting at least one optimal expression system for use in the expression of at least one heterologous recombinant protein, the method comprising: a. obtaining an array assembled using a method comprising: placing in separate addressable locations on the array at least 10 nonidentical test expression systems, said at least 10 nonidentical test expression systems... Learn More