Patent Alert

A U.S. Patent for "Culture Medium for Proliferating a Stem Cell, Which Contains Sulfated Compounds" was awarded to Ajinomoto on June 23rd, 2020. The present invention is a method for culturing a stem cell, comprising culturing the stem cell in a medium comprising: a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof, wherein said sulfated compound or a pharmaceutically acceptable salt thereof is present in said medium at a concentration that promotes the growth of said stem cell in the presence of FGF, and wherein said sulfated compound or pharmaceutically acceptable salt thereof... Learn More

A U.S. Patent for "Data Lineage in an Intelligent Data Integration System" was awarded to Monsanto on June 23rd, 2020. The present invention is a computer-executed method that includes storing in a data store data attributes, data objects, and a data analysis tool (DAT). Each data object has an attribute set and an identifier set. The method includes identifying each data object that has an attribute set comprising a data attribute matching each reference data attribute associated with the DAT, and selecting an identified data object as an input data object for the DAT. The DAT generates a new data object as a function of the input data object... Learn More

A U.S. Patent for "Affinity Chromatography Wash Buffer" was awarded to Cephalon/TEVA on June 23rd, 2020. The present invention is a wash buffer that when used during affinity chromatography purification of a protein of interest, such as an antibody, the wash buffer significantly reduces the level of host cell proteins from the preparation. Following affinity chromatography with the wash buffer, the protein of interest may be further purified using membrane chromatography. Learn More

A U.S. Patent for "Cryopreservation Bags and Method of Use Thereof for Closed System, High Capacity Cell-Banking" was awarded to Boehringer Ingelheim on June 23rd, 2020. The present invention is a high-capacity cryopreservation bag, which is adapted for use in a high capacity biological material, closed cryopreservation banking system, and which is resistant to liquid nitrogen temperatures comprising: a. a first fluorinated ethylene propylene (FEP) enclosure; b. at least two sterile-weldable fluid conduits adapted for use in a high capacity biological material, closed banking system; c. a first rigid FEP inlet conduit, in fluid connection... Learn More

A U.S. Patent for a "Method for Rapidly Screening Microbial Hosts to Identify Certain Strains with Improved Yield and/or Quality in the Expression of Heterologous Proteins" was awarded to Pfenex on June 23rd, 2020. The present invention is a method for selecting at least one optimal expression system for use in the expression of at least one heterologous recombinant protein, the method comprising: a. obtaining an array assembled using a method comprising: placing in separate addressable locations on the array at least 10 nonidentical test expression systems, said at least 10 nonidentical test expression systems... Learn More

A U.S. Patent for a "Customizable Facility" was awarded to Lonza on June 23rd, 2020. The present invention is a campus for fabricating at least one pharmaceutical product, the campus comprising: a plurality of customizable facilities configured to manufacture the at least one pharmaceutical product, the plurality of customizable facilities including a first customizable facility configured to manufacture a first pharmaceutical product and a second customizable facility configured to manufacture a second pharmaceutical product different from the first pharmaceutical product, each customizable facility including: at least one central unit positioned on a floor... Learn More

A U.S. Patent Application for a "Cell Culture Device System and Methods of Use Thereof" was awarded to Oribiotechon June 18th, 2020. The present invention is a device for use in growing, culturing and/or modifying cells, the device comprising a primary container having a base section, a top section arranged substantially in parallel with the base section and a wall element arranged between the top section and the base section and defining an internal lumen of the primary container, in which the wall element of the primary container is compressible with respect to the top and base section, and in which the primary container has at least one optionally sealable inlet... Learn More

A U.S. Patent for a "Device and System for Processing a Liquid Sample Containing Cells" was awarded to Shenzhen Eureka on June 16th, 2020. The present invention is a device for processing a liquid sample containing cells. The device includes a stationary part and a rotatable part. The stationary part includes a cap, a socket connected to the cap, and a tube located within the cap and the socket. The rotatable part includes a centrifugal processing chamber, and a flow guide located within the centrifugal processing chamber. The stationary part and the rotatable part are connected by a plurality of rotary dynamic seals allowing rotation... Learn More

A U.S. Patent for "System and Method for Characterizing Conditions on a Fluid Mixing Device" was awarded to GE on June 16th, 2020. The present invention is a magnetic mixing system to characterize conditions in a fluid mixing device, the system comprising: a mixer vessel comprising a fluid; a drive that creates a magnetic field; a controller that operates the drive; one or more magnet sensors configured to detect the magnetic field or a magnetic flux, and positioned with the system to detect the magnetic field or the magnetic flux; and a processor receiving information from the one or more magnetic sensors... Learn More

A U.S. Patent Application for "Liquid Discharge Device, Sensor Manufacturing Device Having Liquid Discharge Device, and Cell Culture Device Having Liquid Discharge Device" was published by Panasonic on June11th, 2020. The present invention relates to a liquid discharge device for discharging liquid, a sensor device having the liquid discharge device and a cell culture device having the liquid discharge device. The present invention particularly relates to a liquid discharge device suitable for being used for supplying or dispensing a chemical liquid, a reagent, etc., a sensor manufacturing device having the liquid discharge device and a cell culture device... Learn More

A U.S. Patent for "Enhanced Fermentation" was awarded to BASF on June 9th, 2020. The present invention is a method for protecting a microbially produced substance against oxidation, comprising a) providing a microorganism capable of producing said substance, the microorganism comprising, as a heterologous gene, a catalase gene encoding i) a catalase having at least 90% amino acid sequence identity to SEQ ID NO: 1, or ii) a fragment of the catalase according to i), wherein the fragment has catalase activity, and b) cultivating the microorganism under conditions such that said catalase gene is expressed and said substance is produced... Learn More

A U.S. Patent for "Methods for Purifying Antibodies" was awarded to GSK on June 9th, 2020. The present invention is a method for purifying a protein, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising 10 mM to 125 mM sodium caprylate; and c) eluting the protein from the superantigen immobilized on the solid support, wherein the solution is a cell culture feed stream... Learn More

A U.S. Patent for "Abrasion Resistant Film for Biocontainers" was awarded to MilliporeSigma on June 9th, 2020. The present invention is an abrasion resistant, multi-layer material for biocontainers, comprising: a multilayer film, comprising, an outer strength zone, a gas impermeable zone adhered to the outer strength zone, an inner contact zone adhered to the gas impermeable zone, and a porous substrate attached to the outer strength zone, wherein the porous substrate comprises a nylon polymer and is embedded within the outer strength zone; and the porous substrate further comprising: a tie resin layer or a polyethylene layer... Learn More

A U.S. Patent for a "Method of Organizing and Viewing Process Data from Disparate Equipment" was awarded to One Hill Solutions on June 9th, 2020. The present invention is a bioprocess management system comprising: a bioprocess management controller configured to electronically interface one or more bioreactor and one or more analyzer, wherein each bioreactor is configured to maintain a population of cells based on a set of bioreactor operating parameters and to output bioreactor data, wherein each analyzer is configured to analyze at least one sample taken from at least one of the one or more bioreactor, each analyzer further configured... Learn More

A U.S. Patent for "Automated Apparatus and Method of Cell Culture" was awarded to CellProthera on June 9th, 2020. The present invention is an automated apparatus of cell culture, comprising tanks of culture medium, growth factors and cells to be cultured, an incubator with a thermostated enclosure in which is housed a container for cell culture or expansion, and a control computer system including a mechanism configured for entering and recording data intended to control the culture conditions in the enclosure and to manage valves for dispensing fluids in a predefined sequence, wherein it comprises a device for supporting and agitating the cell culture... Learn More

A U.S. Patent for "Control of Protein Glycosylation by Culture Medium Supplementation and Cell Culture Process Parameters" was awarded to Biogen on June 9th, 2020. The present invention is a method of altering the glycosylation pattern of a recombinant glycoprotein produced in cell culture comprising: culturing eukaryotic cells engineered to express a recombinant glycoprotein of interest in a cell culture medium, wherein the cell culture medium is supplemented with an additive comprising mycophenolic acid; wherein the glycosylation pattern of the recombinant glycoprotein of interest is altered relative to the same recombinant glycoprotein... Learn More

A U.S. Patent Application for a "Method for Preparing a Composition Comprising Highly Concentrated Antibodies by Ultrafiltration" was published by Chugai on June 4th, 2020. The present invention is a method for preparing a composition comprising concentrated antibodies by ultrafiltration, wherein the method comprises concentrating an antibody preparation by ultrafiltrating the preparation, said ultrafiltrating comprising: (a) regulating a feed flow rate of the preparation to allow a value of feed pressure applied to an ultrafiltration membrane to increase to 85-100% of a specified maximum feed pressure of the ultrafiltration membrane; and (b) decreasing the feed flow rate... Learn More

A U.S. Patent Application for "Electrochemical Control of Redox Potential in Bioreactors" was published by the University of Colorado on June 4th, 2020. The present invention is a method of producing the fermentation products of mixed culture, the method comprising: a) providing: i) a mixed culture comprising at least two different types of microorganisms; ii) at least one reactor, wherein said reactor comprises at least one chamber, wherein the at least one chamber comprises the mixed culture, at least one anode, and at least one cathode, and wherein the reactor has an open circuit potential; and iii) optionally an ion exchange membrane... Learn More

A U.S. Patent for "Means and Methods for the Generation of Mammalian Producer Cells for the Production of Recombinant Proteins" was awarded to Lonza on June 2nd, 2020. The present invention is a method for the preparation of a protein production engineered mammalian producer cell capable of increasing production of a protein of interest, the method comprising: a) providing a mammalian producer cell; b) increasing the concentration of a combination of miRs selected from the group consisting of miR-15 and miR-34; miR-16 and miR-34; and miR-15, miR-16, and miR-34 in the mammalian producer cell by transfecting the mammalian producer cell... Learn More

A U.S. Patent for "Compositions and Methods for Detecting a Biological Contaminant" was awarded to Regeneron on June 2nd, 2020. The present invention is a method of detecting the presence or absence of a biological contaminant comprising the steps of: (a) combining (i) a positive amplification control plasmid (PAC) sample, wherein the PAC comprises a unique artificial plasmid-specific sequence (UAPS), a target amplification polynucleotide sequence (TAP), and a nucleic acid extraction control (NEC) nucleotide sequence, (ii) a unique detection probe (UDP), (iii) a TAP forward oligonucleotide primer, (iv) a TAP reverse oligonucleotide... Learn More

A U.S. Patent for an "Automated Bioreactor Sampling and Glucose Monitoring System" was awarded to Lewis Ho on June 2nd, 2020. The present invention is an automated bioreactor sampling and glucose monitoring system comprising: a bioreactor containing culture fluid and in fluid connection with a sampling assembly comprising: a holding assembly secured on an open port of the bioreactor to hold a rigid sampling tube to deliver a sample from the bioreactor; a connector connecting the rigid sampling tube and a flexible tubing constructed and arranged outside of the bioreactor, wherein the flexible tubing has a discharging end... Learn More

A U.S. Patent Application for "Mammalian Cells for Producing a Secreted Protein" was published by Boehringer Ingelheim on May 28th, 2020. The present invention relates to the field of cell culture technology. It concerns the knockdown, using RNA interference, or gene knockout, of activating transcription factor 6 beta (ATF6B), or the combination of ceramide synthase 2 (CERS2) and TBC1 domain family member 20 (TBC1 D20) proteins, which play central roles in the cellular secretion pathway. This downregulation leads to improved secretion of biopharmaceutically relevant products produced in mammalian cells... Learn More

A U.S. Patent Application for an "Ejector Equipped Fermenter" was published by Novozymes on May 28th, 2020. The present invention is a fermenter comprising one or more two phase injectors for providing oxygen for the fermentation and a circulation loop, circulating the fermentation broth and providing liquid for the two-phase injectors. Further disclosed is a fermentation plant comprising one or more fermenters of the invention and utility for the fermenters. Also disclosed is the use of the fermenters for the production of a fermentation product such as an enzyme. Learn More

A U.S. Patent for "Electric Pipette System, Electric Pipette and Operating Procedure Display Device" was awarded to the National Institute of Advanced Industrial Science and Technology on May 26th, 2020. The present invention is an electric pipette system, comprising: an operating procedure display device, and an electric pipette the electric pipette comprising: a control unit, which is a micro controller configured to: receive, from the operating procedure display device, an operating condition of a manipulation operation to occur next; control the electric pipette to perform at least one of a suction operation and a discharge operation of a liquid... Learn More

A U.S. Patent for "Microparticles for Cell Disruption and/or Biomolecule Recovery" was awarded to Boehringer Ingelheim on May 26th, 2020. The present invention is a method of manufacturing a protein of interest comprising: (a) cultivating a host cell that produces the protein of interest in a biological fluid; and (b) recovering the protein of interest from the biological fluid by: (i) adding to the biological fluid positively-charged microparticles comprising a ground polymeric anion exchange resin or negatively-charged microparticles comprising a ground polymeric cation exchange resin, and (ii) recovering the proteins of interest from the biological fluid... Learn More

A U.S. Patent for a "Method to Improve Virus Filtration Capacity" was awarded to Genentech on May 26th, 2020. The present invention is a method of virus filtration comprising subjecting a composition comprising a recombinant protein produced in a mammalian host cell and having or suspected of having a parvovirus contaminant to a virus filtration process comprising a cation exchange step and an endotoxin removal step, simultaneously or in either order, immediately preceding a virus filter capable of removing a parvovirus, and wherein said virus filter's filtration capacity in kg/m.sup.2 is improved between 1.5 to 20 fold... Learn More

A U.S. Patent Application for a "System and Method for Regulating Cell Culture Based Production of Biologics" was published by Lonza on May 21st, 2020. The present invention is a system and method for control of at least one bioreactor, other cell cultivation-related equipment, and systems containing any combination of these in a plant. For example, a Plant-Wide Control System (PWCS) or a Process Control System (PCS) may be divided into three main components: hardware (including operating systems, such as a controller communicating with one or more servers of a network associated with the PWCS, (2) software (such as a control module)... Learn More

A U.S. Patent Application for a "System and Method of Bacterial Cell Purification" was published by the Charles Stark Draper Laboratory on May 21st, 2020. The present invention is a method for preparing and processing a sample. The method includes obtaining a sample including biofluid. The method further includes purifying at least part of the sample via an acoustic separator to separate target cells from the sample. The sample may accordingly be at least partially purified. The method further includes causing a portion of an output collected from the acoustic separator to flow through a filter. At least one reagent, such as a lysis reagent or assay reagent, is caused to flow... Learn More

A U.S. Patent for a "Method for Removing Viruses in a Physiologically-Active Protein Containing Sample" was awarded to Chugaion May 19th, 2020. The present invention is a method for removing viruses in a physiologically active protein-containing sample which comprises viruses, which comprises the steps of: 1) applying the physiologically active protein-containing sample which comprises viruses to a protein chromatography column, and eluting, by means of applying an aqueous eluting solution to said column, an eluate comprises said viruses, 2) mixing the eluate of step (1) with an aqueous solution of low conductivity... Learn More

A U.S. Patent Application for "Process Control Systems and Methods for Use with Filters and Filtration Processes" was published by Amgen on May 14th, 2020. The present invention for systems and methods used to control tangential flow filtration are provided, including control systems and methods for use with connected systems with upstream processing units, such as chromatography processing units, in fluid communication with a tangential flow filtration processing unit. Also included are control systems and methods for performing continuous concentration using single-pass tangential flow filtration with permeate flow control. Learn More

A U.S. Patent Application for a "Novel Method of Protein Purification" was published by Ansun Biopharma on May 14th, 2020. The present invention is a composition comprising a polypeptide comprising the amino acid sequence of SEQ ID NO: 1 and 2 made by the method comprising: (a) providing a culture of a gram negative bacterial cell expressing the intracellular protein of interest; (b1) contacting the culture with an inorganic salt; holding the culture containing the added inorganic salt for at least 10 minutes; contacting the culture containing the added inorganic salt with a chelating agent; or (b2) contacting the culture with an inorganic salt... Learn More

A U.S. Patent Application for an "Automated System for HTP Genomic Engineering" was published by Zymergen on May 14th, 2020. The present invention is an automated system for improving the phenotypic performance of a microbial strain, the system comprising: a plurality of modules, wherein the modules of the system are configured and arranged to: a. provide a parental lineage microbial strain and a production microbial strain derived therefrom, wherein the production microbial strain comprises a plurality of identified genetic variations selected from single nucleotide polymorphisms, DNA insertions, and DNA deletions... Learn More

A U.S. Patent for "Methods of Controlling the Formation of Disulfide Bonds in Protein Solutions" was awarded to Genzyme on May 12th, 2020. The present invention is a method of controlling the number of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess, the method comprising: (a) contacting the polypeptides with a conditioned solution at a specific time point during the bioprocess, wherein the conditioned solution comprises a redox reagent, and (b) incubating the conditioned solution comprising the polypeptides for a predetermined time at a predetermined temperature; wherein the incubation of the polypeptides with the conditioned solution controls the formation of disulfide bonds between the polypeptides of the protein; wherein the number of disulfide bonds between the polypeptides of the protein is increased... Learn More

A U.S. Patent for "High Concentration Protein Formulations with Reduced Viscosity" was awarded to the Bhami Research Lab on May 12th, 2020. The present invention is an antibody agent formulation comprising: (i) one or more antibody agents at a concentration between about 10 mg/mL and about 300 mg/mL; and (ii) a viscosity-reducing agent that comprises a combination of nicotinic acid (acid form) and tryptophan, present at a concentration between about 5 mM and about 100 mM each, wherein: the formulation has a viscosity between about 1 cP and about 100 cP when measured at 25.degree. C., using a cone and plate viscometer. Learn More

A U.S. Patent for a "Cyclic Filtration System" was awarded to Georgia Tech on May 12th, 2020. The present invention is a method of filtering a fluid with components includes providing an alternating pressure. The alternating pressure yields an oscillating transmembrane pressure through volume and pressure variations within a filtration chamber while sealing the filtration chamber. A separation surface can be housed in the filtration chamber wherein an influent is introduced. Components can be concentrated on the separation surface, effectively removing some or all of them from the fluid... Learn More

A U.S. Patent for "Microbial Strain Improvement by a HTP Genomic Engineering Platform" was awarded to Zymergen on May 12th, 2020. The present inventionprovides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest. Learn More

A U.S. Patent Application for a "Cloud-Controlled Manufacturing Execution System (CLO-cMES) for Use in Pharmaceutical Manufacturing Process Control, Methods, and Systems Thereof" was published by SMP Logic Systems on April 30th, 2020. The present invention is a "Cloud" based manufacturing execution systems ("MES") and methods thereof used to control, execute, and monitor pharmaceutical or biopharmaceutical production processes and systems are disclosed herein. Consequently, the methods and systems provide a means to quality manufacturing on an integrated level whereby drug or biologic manufacturers can achieve data and product integrity and ultimately minimize cost... Learn More

A U.S. Patent Application for a "Culture Device and Culture Method" was published by Kyoto Seisakusho on April 30th, 2020. The present invention is a culture apparatus comprising: a culture vessel including an internal space containing a culture solution in which a culture target is suspended; a gas supply apparatus supplying a single gas or a mixed gas to the internal space of the culture vessel; and an agitation apparatus agitating the culture solution by changing position and posture of the culture vessel, wherein the agitation apparatus changes the position and posture of the culture vessel to circulate the culture solution in the internal space of the culture vessel... Learn More

A U.S. Patent Application for "Bioreactors with Multiple or Adjustable Position Agitator Designs" was published by Genentech on April 30th, 2020. The present invention described herein are bioreactor support structures configured to be used with containers having different designs, and methods for making and using such bioreactors. The support structures described herein may include two or more agitator motors and control systems or an adjustable-position agitator motor, a removable spacer and/or lid, multiple configurations for ports and probes, and multiple exhaust filter heating blankets. Also described herein are methods of manufacturing a multi-agitator motor or adjustable-position agitator motor... Learn More

A U.S. Patent for "Tandem Folding Methods to Improve Protein Folding Yield" was awarded to Purdue University on April 28th, 2020. The present invention provides a method of refolding a solubilized cysteine-containing protein to its native form, the method comprising adding a reversible reducing agent to a solution containing the solubilized cysteine-containing protein to initiate folding of the cysteine-containing protein, wherein the reversible reducing agent is added at a ratio of : of 3:1 to 4:1; and adding an irreversible reducing agent to the solution at a time after the initiation of folding to breakdown aggregates of the cysteine-containing protein. Learn More

A U.S. Patent for "Production Methods" was awarded to GSK on April 28th, 2020. The present invention provides methods of reducing the levels of a titratable selectable pressure required, the number of amplification cycles, and the time taken to generate protein expressing cell lines by altering the codons of the desired open-reading-frames. Through the use of codon adaptation for this purpose the methods of the invention consistently provide sufficient yields in faster time frames saving many weeks in cell line development activities. Furthermore the methods of the invention also generate cell lines with lower concentrations of selection and amplification agent than previously achievable... Learn More

A U.S. Patent Application for "Defect Detection in Lyophilized Drug Products with Convolutional Neural Networks" was published by Genentech on April 23rd, 2020. The present invention is a method comprising, by a computing system: receiving one or more querying images associated with a container of a pharmaceutical product, wherein each of the one or more querying images is based on a particular angle of the container of the pharmaceutical product; calculating, for the container of the pharmaceutical product, one or more confidence scores associated with one or more defect indications, respectively, by processing the one or more querying images... Learn More

A U.S. Patent for a "Method for Increasing the Glutathione Level in Cells" was awarded to Merck KGaA on April 21st, 2020. The present invention is a method for increasing the level of glutathione in mammalian cells, comprising adding to said cells an amount of S-sulfocysteine and/or a S-sulfocysteine salt effective to increase the intracellular glutathione level... Learn More

A U.S. Patent for a "Method of Protein Manufacture" was awarded to UCB Biopharma on April 21st, 2020. The present invention is a method for manufacturing an antigen-binding fragment of an antibody coupled to a polyethylene glycol (PEG) molecule, comprising: a) culturing prokaryotic host cells under conditions such that the cells express the antigen-binding fragment of an antibody, b) purifying said antigen-binding fragment from a mixture containing the host cells and other contaminants, wherein said purification comprises at least two chromatography steps and wherein a reducing agent is added to said mixture... Learn More

A U.S. Patent Application for "Selective Reduction of Proteins" was published by Seattle Genetics on April 16th, 2020. The present invention provides a method for making uncapped cysteine protein preparations, including uncapped engineered cysteine antibody preparations. The methods include, inter alia, contacting a reducing agent with engineered cysteine antibody molecules, each of the antibody molecules having at least one capped engineered cysteine residue and at least one interchain disulfide bond and reacting the reducing agent with the antibody molecules under conditions sufficient to uncap engineered cysteine residues and form cap byproducts... Learn More

A U.S. Patent for "Removal of Residual Cell Culture Impurities" was awarded to Novartis on April 14th, 2020. The present invention is a method for removing Influenza Nuclear Protein (NP) from a preparation comprising virus proteins of interest, the method comprising the steps of: a. splitting a virus preparation derived from cell culture or eggs, b. adding an anionic detergent to the virus preparation under non-precipitating conditions whereby no substantial precipitation of viral proteins or DNA occurs, and c. processing the virus preparation through an ion exchange matrix, whereby the nuclear protein is bound... Learn More

A U.S. Patent for "Yeast Promoters from Pichia Pastoris" was awarded to Keck on April 14th, 2020. The present invention is an isolated nucleic acid wherein the sequence of the isolated nucleic acid comprises a sequence at least 95% identical to SEQ ID NO:2, or at least 95% identical to a fragment thereof, wherein the isolated nucleic acid comprises the sequence of a constitutive Pichia pastoris promoter operably linked to a heterologous coding sequence, and wherein the fragment extends about 300 nucleotides, about 400 nucleotides, about 500 nucleotides, about 600 nucleotides, or about 700 nucleotides upstream from the 3' end of SEQ ID NO:2... Learn More

A U.S. Patent for "On-Column Viral Inactivation Methods"  was awarded to Sanofi  on April 7th, 2020.  The present invention is a method of inactivating a virus present during production of a polypeptide of interest, the method comprising the following sequential steps: (a) binding the polypeptide to a chromatography matrix, (b) washing the polypeptide-bound chromatography matrix of step (a) with a first wash solution comprising a salt at a pH above 6.5, (c) washing the polypeptide-bound chromatography matrix of step (b) with a second wash solution comprising a salt at a concentration greater than about 0.5 M... Learn More

A U.S. Patent for "Methods and Systems for Chromatography Data Analysis" was awarded to Regeneron on April 7th, 2020. The present invention is a process control method, comprising: receiving raw chromatography data including a plurality of signals, wherein each signal of the plurality of signals is associated with one of a plurality of blocks; obtaining a subset of data by selecting a combination of a first block and a first signal from the raw chromatography data; generating processed chromatography data by applying a noise reduction technique to the subset of data, wherein applying the noise reduction technique includes... Learn More

A U.S. Patent for "Methods for Viral Inactivation Using Eco-Friendly Detergents" was awarded to Genentech on April 7th, 2020. The present invention provides for methods of inactivating virus in product feedstream in a manufacturing process of a therapeutic polypeptide using an environmentally compatible detergent. The environmentally compatible detergent does not adversely affect product quality while effectively inactivating virus in the feedstream. Learn More

A U.S. Patent for "Production Cell Line Enhancers" was awarded to Regeneron on April 7th, 2020. The present invention relates to discovery of the ectopic expression of EDEM2 in a production cell to improve the yield of a useful multi-subunit protein. Thus, the present invention provides for production cell lines, such as the canonical mammalian biopharmaceutical production cell, the CHO cell, containing recombinant polynucleotides encoding EDEM2. Also disclosed is a production cell containing both an EDEM2-encoding polynucleotide as well an XBP1-encoding polynucleotide. Improved titers of antibodies produced by these cell lines are disclosed... Learn More

A U.S. Patent Application for "Real-Time Prediction of Chemical Properties Through Combining Calculated, Structured and Unstructured Data at Large Scale" was published by IBM on April 2nd, 2020. The present invention is a method in a data processing system comprising at least one processor and a memory comprising instructions which, when executed by the at least one processor, causes the at least one processor to implement a real-time prediction engine for real-time predication of chemical properties through combining calculated, structured, and unstructured data at large scale, the method comprising... Learn More

A U.S. Patent Application for "Vaccine Compositions Having Improved Stability and Immunogenicity" was published by Novavax on April 2nd, 2020. The present invention is a method of stimulating an immune response in a subject comprising administering a vaccine composition comprising: (i) a nanoparticle comprising a non-ionic detergent core and a viral glycoprotein, wherein the viral glycoprotein is associated with the core; wherein the non-ionic detergent is PS80; wherein the viral glycoprotein is an RSV F glycoprotein and the molar ratio of PS80 to viral glycoprotein is about 30:1 to about 60:1; and (ii) a pharmaceutically acceptable buffer. Learn More

A U.S. Patent Application for "Ion Exchange Membrane Chromatography" was published by Genentech on April 2nd, 2020. The present invention are methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective... Learn More

A U.S. Patent Application for "Compositions, Methods, and Systems for Affinity Based Protein Identification and Purification" was published by Recombipure on April 2nd, 2020. The present invention disclosed herein are compositions, methods, and systems for the purification and/or detection of recombinant and other proteins. In some embodiments, compositions may comprise recombinant protein with one or more sequences having substantial homology to RP-Tag Small or RP-Tag Large. In some cases, the disclosed compositions may be useful in binding or recognizing target proteins. Learn More

A U.S. Patent for "Adsorptive Membranes for Trapping Viruses" was awarded to the Wisconsin Alumni Research Foundation on March 31st, 2020. The present invention is a disposable, virus-trapping membrane, and a corresponding method to remove viruses from solution are described. The membrane includes a disposable, micro-porous filter membrane and a ligand immobilized on the membrane. The ligand irreversibly and selectively binds viruses. The ligand also has a pKa sufficiently high to repel antibodies via electrostatic charge repulsion. Learn More

A U.S. Patent for "pH Adjustment to Improve Thaw Recovery of Cell Banks" was awarded to Genentech on March 31st, 2020. The present invention is a method of improving thaw recovery of cell banks comprising freezing CHO cells for banking in a freezing medium, wherein the freezing medium comprises a buffered solution and a cryoprotective agent, and wherein the freezing medium has a pH of 7.7 to 8.3 prior to freezing. Learn More

A U.S. Patent for "Materials and Methods for Extended Continuous Flow Fermentation of Reduced Genome Bacteria" was awarded to Scarab Genomics on March 31st, 2020. The present invention is a continuous fermentation process for the production of a biological product comprising culturing a population of reduced genome Escherichia coli (E. coli) bacteria cells comprising an inducible expression vector encoding a biological product in at least two successive fermentors, each configured as an independent continuous flow chemostat, said fermentors comprising a first fermentor comprising culture medium... Learn More

A U.S. Patent for "Host Cells for Use in an Inducible Coexpression System" was awarded to AbSci on March 31st, 2020. The present invention is a host cell suitable for expression of at least one gene product from two or more inducible promoters, wherein at least one of said inducible promoters is responsive to an inducer that is different than the inducer of another of said inducible promoters, and each inducible promoter is selected from the group consisting of: an L-arabinose-inducible promoter, a propionate-inducible promoter, a rhamnose-inducible promoter, and a D-xylose-inducible promoter; wherein the host cell has an altered gene function... Learn More

A U.S. Patent for "Isolating Cells Expressing Secreted Proteins" was awarded to Regeneron on March 24th, 2020. The present invention is a method of high expression of a protein of interest, comprising: (a) transfecting a mammalian cell with (i) a nucleic acid encoding the protein of interest and (ii) a nucleic acid encoding a cell surface capture molecule that comprises a membrane anchor and is capable of binding the protein of interest; (b) culturing the mammalian cell under conditions in which the protein of interest and cell surface capture molecule are expressed, and a protein of interest-cell surface capture molecule complex is formed and displayed on the cell surface of the mammalian cell... Learn More

A U.S. Patent Application for "Methods, Apparatuses, and Systems for Continuously Inactivating a Virus During Manufacture of a Biological Product " was published by Boehringer Ingelheim on March 19th, 2020. The present invention is an apparatus for continuously inactivating a virus during manufacture of a biological product, the apparatus comprising: an initial-mixing vessel; a pre-treatment detector chamber; a pre-treatment hold reservoir; a drain valve; and a treatment vessel that comprises an inlet, an outlet, and a static mixer, the inlet and the outlet being positioned at opposite ends of a major axis of the treatment vessel... Learn More

A U.S. Patent Application for "Methods for Increasing Mannose Content of Recombinant Proteins" was published by Amgen on March 19th, 2020. The present invention is a method for modulating mannose 5 on a recombinant protein during a mammalian cell culture process comprising: establishing a cell culture of mammalian cells in a cell culture medium, wherein the mammalian cells produce a recombinant protein; limiting the amount of glucose in the cell culture medium, wherein the concentration of the glucose is from about 0 to 6 g/L; and supplementing the cell culture medium with galactose or sucrose... Learn More

A U.S. Patent for "Recombinant Host Cell for Expressing Proteins of Interest" was awarded to Boehringer Ingelheim on March 17th, 2020. The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products. Learn More

A U.S. Patent for "Compositions for Cell Culture and Methods of Using the Same" was awarded to Bristol Myers Squibb on March 17th, 2020. The present invention is a cell culture process for the production of protein, comprising: a) culturing Chinese Hamster Ovary (CHO) cells which produce a protein of interest in cell culture under conditions that allow for protein production; and b) adding one or more antioxidant selected from the group consisting of catechin, chlorogenic acid, pelargonidin, quercetin, resveratrol, rosmarinic acid and silibinin to the cell culture, wherein protein titer is increased compared to the same cell culture without the antioxidant supplementation. Learn More

A U.S. Patent Application for "Mammalian Cell Cryopreservation Liquid" was published by Otsuka Pharma on March 12th, 2020. The present invention is a liquid for cryopreserving a cell and a liquid for administration of a mammalian cell capable of cryopreserving a mammalian cell and effectively suppressing cell death after thawing, and a method for cryopreserving a mammalian cell using the cell cryopreservation liquid. The liquid is an isotonic solution that includes 2.0 to 6.0% (w/v) of trehalose or a derivative thereof, or a salt of the trehalose or the derivative, 4.0 to 7.0% (w/v) of dextran or a derivative thereof... Learn More

A U.S. Patent Application for "Feed Media" was published by Amgen on March 12th, 2020. The present invention is a method for stabilizing a concentrated feed medium for feeding a mammalian cell culture comprising including in the feed medium at least about 9 mM pyruvate, wherein the feed medium comprises cysteine and/or cystine, wherein the sum of the concentrations of cysteine and/or cystine is at least about 7.9 mM, wherein the pH of the feed medium is from about 5.8 to about 7.4, wherein the feed medium comprises tyrosine and the tyrosine concentration is not more than about 4.4 mM, and wherein the feed medium with the included pyruvate is stable... Learn More

A U.S. Patent Application for "Methods for Cryogenic Storage" was published by Juno Therapeutics on March 12th, 2020. The present invention is a method comprising cryogenically storing cells from a biological sample derived from a donor, wherein the cells are obtained from the donor at a point in time that is after the donor is diagnosed with, or deemed to have or be suspected of having, a disease or condition, and before the donor has received one or more treatments for the disease or condition; and wherein the cells are frozen in a controlled rate freezer using a stepwise freezing profile comprising at least one step wherein the sample and/or chamber is cooled at a rate greater than 1.degree. C. per minute. Learn More

A U.S. Patent Application for "Microcarrier Perfusion Culturing Methods and Uses Thereof" was published by Genzymeon March 12th, 2020. The present invention is a method for testing a manufacturing process for making a recombinant protein, the method comprising: providing a shake tube containing an adherent Chinese hamster ovary (CHO) cell containing a nucleic acid encoding a recombinant protein disposed in a first liquid culture medium, wherein the first liquid culture medium occupies about 10% to about 30% of the volume of the shake tube and contains a plurality of microcarriers at a concentration of about 1.0 g/L to about 15.0 g/L... Learn More

A U.S. Patent for "Process Control Systems and Methods for Use with Filters and Filtration Processes " was awarded to Amgenon March 10th, 2020. The present invention is a process control system comprising: (a) one or more filtration or purification processing units each operating at a flow rate, the one or more processing units comprising at least one of filtration or purification processing units; (b) a tank connected downstream from the one or more processing units; (c) a filter having an inlet, a permeate outlet, and a retentate outlet connected to the tank; (d) a feed pump having an inlet connected to the tank and an outlet connected to the inlet of the filter... Learn More

A U.S. Patent for a "In Vivo Production of Proteins" was awarded to Moderna on March 10th, 2020. The present invention is a method of expressing a protein in a mammalian subject, the method comprising administering a pharmaceutical composition comprising a plurality of lipid nanoparticles encapsulating a polynucleotide, wherein the lipid nanoparticle comprises a biodegradable cationic lipid, a neutral lipid, cholesterol, and a PEGylated lipid and the plurality of lipid nanoparticles has a mean particle size of between 80 nm to 160 nm, and wherein the polynucleotide comprises: (a) an open reading frame encoding the protein consisting of nucleotides selected... Learn More

A U.S. Patent for a "Cell Culture Process" was awarded to Momenta Pharma on March 10th, 2020. The present invention is a method of manufacturing a preparation of adalimumab, comprising: culturing a cell in a medium comprising 1.5 g/L arginine to 20 g/L arginine under conditions in which the cell expresses adalimumab; isolating the adalimumab, thereby producing a preparation of adalimumab; and formulating the preparation into a drug product if the preparation meets a target value of one or more C-terminal variants of adalimumab, wherein the C-terminal variants differ in amino acid sequence only by the presence or absence of a lysine... Learn More

A U.S. Patent Application for "End to End Cell Therapy Automation" was published by Lonza on March 5th, 2020. The present invention is a method for automated production of a genetically modified immune cell culture, the method performed by a cell engineering system, comprising: (a) activating an immune cell culture with an activation reagent to produce an activated immune cell culture in a first chamber of the cell engineering system; (b) transducing the activated immune cell culture, the transducing comprising: i. transferring via a first sterile, closed connection, the activated immune cell culture from the first chamber to an electroporation unit... Learn More

A U.S. Patent Application for "Sterile Chromatography Resin and Use Thereof in Manufacturing Processes" was published by Genzyme on March 5th, 2020. The present invention are methods of reducing bioburden of a chromatography resin that include exposing a container including a composition including (i) a chromatography resin and (ii) a liquid including at least on alcohol to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one alcohol are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Learn More

A U.S. Patent for "Capture Purification Processes for Proteins Expressed in a Non-Mammalian System" was awarded to Amgen on March 3rd, 2020. The present invention are methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided. Learn More

A U.S. Patent for "Methods of Perfusion Culturing Using a Shake Flask and Microcarriers" was awarded to Genzyme on March 3rd, 2020. The present invention is a method of optimizing a manufacturing process of producing a recombinant protein, the method comprising: providing a shake flask containing a mammalian cell disposed in a first liquid culture medium, wherein the first liquid culture medium occupies about 20% to about 30% of the volume of the shake flask and contains a plurality of microcarriers at a concentration of about 1.0 g/L to about 15.0 g/L; incubating the shake flask for a period of time at about 32.degree. C. to about 39.degree. C.... Learn More

A U.S. Patent for "Compositions and Methods for Protein Glycosylation" was awarded to GSK on March 3rd, 2020. The present invention is oligosaccharyl transferases for use in N-glycosylating proteins of interest in vitro and in host cells. Methods for using such oligosaccharyl transferases, nucleic acids encoding such oligosaccharyl transferases, and host cells comprising such oligosaccharyl transferases are also provided herein. Glycoconjugates generated by using such oligosaccharyl transferases are also provided herein. Learn More

A U.S. Patent Application for "Growth Control of Eukaryotic Cells" was published by Roche on February 27th, 2020. The present invention is a system configured to receive a target cell density and a target time and for each of a plurality of time intervals; receive a measured cell density of the eukaryotic cells in the culture medium of the first tank; compute a predicted cell density at target time as a function of at least the measured cell density; compare the predicted cell density and the target cell density; adjusting the temperature of the culture medium in the first tank if the predicted cell density deviates from the target cell density by increasing the temperature by 0.1.degree... Learn More

A U.S. Patent Application for "Use of Raman Spectroscopy in Downstream Purification" was published by Regeneronon February 27th, 2020. The present invention are Raman spectroscopy methods and systems for characterizing or quantifying a protein purification intermediate and/or final concentrated pool during production or manufacture are provided. In one embodiment, in situ Raman spectroscopy is used to characterize or quantify protein purification intermediates critical quality attributes during downstream processing (i.e., after harvest of the protein purification intermediate). For example, the disclosed in situ Raman spectroscopy methods and systems... Learn More

A U.S. Patent for "Protein Separations Using an Acrylamide Containing Filter" was awarded to MilliporeSigma on February 25th, 2020. The present invention is a method of improving the filtration capacity of a virus filter during purification of a protein of interest, comprising subjecting a sample comprising said protein of interest to a prefiltration step prior to passing through said virus filter, said prefiltration step comprising contacting said sample with a porous solid support selected from the group consisting of chromatographic resin, a membrane, a porous bead, a porous monolith, a winged fiber, a woven fabric and a non-woven fabric... Learn More

A U.S. Patent for "Rapid Screening of Monoclonal Antibodies" was awarded to Cellply on February 25th, 2020. The present invention is a method for screening an antibody for a desired response comprising the steps of: a) making available one or more inverted open microwells, wherein a microchannel having a cross sectional area of micrometer dimensions provides fluid to the one or more inverted open microwells, the microchannel having, at a first end, a fluid inlet, at a second end, a fluid outlet, wherein the fluid flows in a direction from the fluid inlet to the fluid outlet, wherein each of the one or more inverted open microwells... Learn More

A U.S. Patent for a "System and Method for Regulating Cell Culture Based Production of Biologics" was awarded to Lonza on February 25th, 2020. The present invention is a system for performing real-time analysis on a chromatography column comprising: a chromatography column; a conductivity meter; and at least one processor for executing stored program instructions to: measure one or more conductivity values of the chromatography column using the conductivity meter, determine a baseline conductivity value by recording a predetermined number of conductivity values from a first column volume to a second column volume and calculating an average... Learn More

A U.S. Patent for "Seed Train Processes and Uses Thereof" was awarded to Genzyme on February 25th, 2020. The present invention is a method of producing a recombinant protein, wherein the method consists of: (a) disposing a plurality of recombinant mammalian cells into a first culture medium within a vessel to provide a first cell culture; (b) batch culturing the first cell culture to a cell density range of about 1.0.times.10.sup.6 cells/mL to about 5.0.times.10.sup.6 cells/mL; (c) disposing a volume of the first cell culture of step (b) into a second culture medium within a perfusion bioreactor to provide a second cell culture with an initial cell density... Learn More

A U.S. Patent for a "Process for Preparing Recombinant Insulin Using Microfiltration" was awarded to Merck on February 18th, 2020. The present invention is a process for producing insulin or insulin analog, wherein the process has a digestion step in which an aqueous solution of properly refolded pre-proinsulin or pre-proinsulin analog is digested with one or more proteolytic enzymes to produce an aqueous solution of insulin or insulin analog, digestion byproducts, and host cell impurities and a downstream purification or chromatography step, in which the insulin or insulin analog is separated from the digestion byproducts, and host cell impurities... Learn More

A U.S. Patent for "Bioreactors with Multiple or Adjustable-Position Agitator Designs" was awarded to Genentech on February 18th, 2020. The present invention described herein are bioreactor support structures configured to be used with containers having different designs, and methods for making and using such bioreactors. The support structures described herein may include two or more agitator motors and control systems or an adjustable-position agitator motor, a removable spacer and/or lid, multiple configurations for ports and probes, and multiple exhaust filter heating blankets... Learn More

A U.S. Patent for "Cross-Scale Modeling of Bioreactor Cultures Using Raman Spectroscopy" was awarded to Biogen on February 18th, 2020. The present invention is a method of assessing a bioreactor culture, the method comprising: (i) obtaining a Raman spectrum of a manufacturing-scale bioreactor culture using a probe configured inline with the bioreactor; and (ii) determining a culture parameter of the manufacturing-scale bioreactor culture using a multivariate partial least squares (PLS) model that relates the Raman spectrum to the culture parameter, wherein the model was developed based on one or more test bioreactor cultures... Learn More

A U.S. Patent for "Methods for Quantifying Particulates in Cell Culture" was awarded to Celularity on February 18th, 2020. The present invention are methods for quantifying and/or detecting sub-visible particulates in cell cultures. The methods provided herein allow for the determination of the presence of non-cell sub-visible particulates in cell cultures, i.e., sub-visible particulates that are not intact cells, and quantification of such sub-visible particulates. The methods provided herein comprise a step of breaking down, e.g., lysing, cells in a cell culture. By breaking down the cells in the cell culture, non-cell sub-visible particulates can readily be detected... Learn More

A U.S. Patent for "Cell Culture Media" was awarded to Merck KGaA on February 18th, 2020. The present invention relates to dry cell culture media comprising amino acid components of certain particle size. Some dry powder cell culture media show poor dissolving properties and result in turbid solutions when they are dissolved in aqueous solutions. Using amino acid components of certain particle sizes significantly reduces that problem. Learn More

A U.S. Patent for an "Auto-Sampling System for Automatically Acquiring a Sample" was awarded to Waters on February 11th, 2020. The present invention is a sampling system comprising an external sampling assembly in fluidic communication with a process sample manager is provided herein. The sampling system can automatically acquire sample from one or more sources of sample to prepare sample for injection into a column or detector. The external sampling assembly has an external sampling valve connected to an external pump and is in fluidic communication with a process sample manager. The external sampling valve... Learn More

relates to monovalent influenza vaccine formulations and vaccination regimes for immunising against influenza disease, their use in medicine, in particular their use in augmenting immune responses to various antigens, and to methods of preparation. In particular, the invention relates to monovalent influenza immunogenic compositions comprising an influenza antigen or antigenic preparation thereof from an influenza virus strain being associated with a pandemic outbreak or having the potential to be associated with a pandemic outbreak... Learn More

A U.S. Patent for "Expanding Cells in a Bioreactor" wasawarded to Terumo BCT on February 11th, 2020. The present invention is a system for expanding cells, the system comprising: a bioreactor; at least one motor configured to connect to and rotate the bioreactor; at least one fluid circulation path fluidly associated with the bioreactor; at least one pump for circulating fluid through the at least one fluid circulation path and the bioreactor; a processor configured to execute processor executable instructions; and a memory storing processor executable instructions that when executed by the processor perform a method comprising... Learn More

A U.S. Patent for "Selection Marker for Cell Transfection and Protein Production" was awarded to Sanofi on February 11th, 2020. The present invention is within the field of industrial protein production. The invention provides a novel expression system using dihydroorotate dehydrogenase (DHODH) as a selection marker in combination with leflunomide or a metabolite thereof, notably for use in mammalian cell lines. Expression vectors encoding DHODH, cell lines comprising said vectors and methods of producing recombinant proteins are also provided. Learn More

A U.S. Patent for "pH Responsive Mucoadhesive Polymeric Encapsulated Microorganisms" was awarded to MIT on February 4th, 2020. The present invention is a method of encapsulating microorganisms, or components thereof, for targeted enteric delivery to an animal host have been developed. In particular, the encapsulation provides a prolonged survival, extended retention and a pH-sensitive release of the encapsulated microorganisms or antigenic components thereof at targeted sites within the gastrointestinal tract. The formulations are useful for diagnostic, therapeutic and prophylactic purposes and can alter a host's microbial composition associated with a condition or a disease state. Learn More

A U.S. Patent for "Primary Cell Gene Editing" was awarded to PACT Pharmaon February 4th, 2020. The present invention is a method of treating a cancer in a human patient comprising: a. modifying a patient-derived T cell by a nuclease-mediated introduction of a non-viral polynucleotide into the T cell, wherein the non-viral polynucleotide comprises: i. first and second homology arms homologous to first and second endogenous sequences of the cell; ii. a TCR gene sequence positioned between the first and second homology arms; iii. a first P2A-coding sequence positioned upstream of the TCR gene sequence... Learn More

A U.S. Patent for "Waveguide-Based Optical Scanning Systems" was awarded to LDIP on February 4th, 2020. The present invention is a scanning sensor system, methods of use and kits for detecting a biologically active analyte are provided. The scanning sensor system includes a light source, a detector, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, and at least one adapter configured to couple with the substrate and provide optical communication between the light source, the waveguides of the substrate, and the detector. Learn More

A U.S. Patent for "Methods for Increasing the Capacity of Flow-Through Processes" was awarded to MilliporeSigma on February 4th, 2020. The present invention is a process for separating target proteins from non-target proteins, comprising: a) obtaining a liquid sample containing target proteins and non-target proteins; b) increasing the concentration of the target proteins and non-target proteins in the sample by an ultrafiltration process to produce a concentrated feed for delivery to a chromatography device, the ultrafiltration process being a recirculating tangential flow filtration (TFF) process, a single-pass TFF (SPTFF) process, or a TFF process... Learn More

A U.S. Patent for a "Method of Cell Culture" was awarded to Pfizer on January 28th, 2020. The present invention is a method of cell culture comprising (i) providing cells in a cell culture medium to start a fed-batch cell culture process, and, (ii) maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenyl pyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3mM, wherein maintaining the at least one metabolite below a concentration C1 in the cell culture comprises the step... Learn More

A U.S. Patent for a "Disposable Liquid Chemical Sensor System" was awarded to Entegris on January 28th, 2020. The present invention is a disposable sensor system comprising: at least one disposable sensor assembly comprising a threaded cap with an index of refraction sensor bonded to the threaded cap, said index of refraction sensor measures a chemical concentration based on a refractive index, said sensor configured to perform data acquisition representing a chemical concentration, the disposable sensor assembly is affixed through a port assembly to a disposable polymeric bag container, the disposable polymeric bag comprising an optical window integrated... Learn More

A U.S. Patent for "Methods of Replicating a Large Scale Eculizumab Production Cell Culture" was awarded to Alexion on January 28th, 2020. The present invention is a method of replicating a large-scale eculizumab production cell culture in a small scale culture, the method comprising: adding 3.7 mL of a first lipid solution containing linoleic acid, oleic acid and cholesterol prior to inoculation; providing a 4-L to 10-L fed batch bioreactor containing a first culture medium that occupies between about 70% and about 90% of the capacity of the bioreactor and has an initial cell density of between about 2.5.times.10.sup.5 and about 7.5.times.10.sup.5 NS0 cells/mL... Learn More

A U.S. Patent for "Bioreactors and Uses Thereof" was awarded to Biostage on January 28th, 2020. The present invention is a bioreactor comprising: vessel having side walls defining a chamber, the side walls having a first end and a second end opposed to the first end, the chamber having at least one opening that communicates with the chamber, the at least one opening defined proximate to the first end of the side walls, the chamber being configured for containing a biological object under culture conditions; a cover component configured for covering the at least one opening located proximate to the first end of the side walls, the cover component removably connected to the vessel...
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A U.S. Patent for a "Method of Cell Culture" was awarded to Pfizer on January 28th, 2020. The present invention isa method of cell culture comprising (i) providing cells in a cell culture medium to start a fed-batch cell culture process, and, (ii) maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenyl pyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3mM, wherein maintaining the at least one metabolite below a concentration C1 in the cell culture comprises. Learn More