Solution Alert

It took a little digging, but I think I have a couple of products from industry leaders that will enable both your optical dissolved oxygen probes, as well as your conventional pH sensors, to be used with your single-use bags. The beauty of both these products is that they allow you to use sensing solutions that have been proven accurate and reliable in bioprocessing while at the same time fulfilling your need for them to support applications in single-use bags.


Mettler Toledo

It sounds like your colleague got a look at the Seg-Flow S3 from Flownamics, which according to my sources was featured extensively at this year's ESACT conference in Copenhagen. While I have not seen the system first-hand it is my understanding that, while based on the well-known Seg-Flow system, it features a number of improvements in design and functionality. Here is a link to more information as well as images;
Seg-Flow S3

You definitely know what you want, or don't want as the case may be, and I think I have found it. A 202-page doctoral of engineering thesis prepared at University College London with the support not of the manufacturer but of no less an independent source than Eli Lilly. Complete with chapters dedicated to fluid mixing, mass transfer and so much more.

Engineering Characterization of a Rocked Bag Bioreactor for Improved Process development and Scale-Up

You had me worried for a second there, I thought for sure there was no way I was going to be able to get you where you wanted to go. Thankfully your reference to 'Boss' made me realize you had seen the 'Ross' single-use bag storage solution from the folks at Single Use Support. I have included a link to it below.

While I was not at Interphex this year, I'm happy to report that I know of that which you speak. The system in question is the Applikon V-Control, a successful collaboration between Applikon and the folks at Emerson to seamlessly integrate DeltaV with Applikon's suite of solutions for bioprocessing. It is my understanding that the platform can manage as many as 32 bioprocesses at scales as low as 250mL to upwards of 50L. The link below will take you to additional information.


In the interest of clarity, it should be noted that in this instance what is meant by single-use is both the components into which the sample is delivered as well as the sample valve itself. That being clear, there are no fewer than four such solutions I can suggest you take a look at. All have more or less similar functionality, the use of needles for sampling, with the exception of one which is needless.

Gore Aseptic Sampling Valve

MilliporeSigma NovaSeptum

Sartorius TakeOne

Thermo Scientific Three60

That is one heck of a catch, but you are in luck. For some years now there has been a family of sensors, available in hand held and mounted versions, made by the folks at Bug Lab that meet the requirements you mention. In your question you specify non-invasive, both the hand held and mounted versions of these sensors meet that requirement, so the link below will take you to more information about each.

Bug Lab

I recently came across a White Paper that might be of value to you. It highlights the ability of a new system that utilizes laser induced fluorescence to conduct real-time microbial monitoring of Purified Water, Ultrapure Water and WFI. The result of which being the real-time release of these waters for use in your bioprocess. The link below will take you to the White Paper.

Microbial Monitoring Improves Bioprocess Control

I think the event you are referring to was the bioprocess optimization seminar organized by Flownamics that took place in Cambridge, MA on February 28th. If that sounds about right, then the instrument in question is the Online Protein Analyzer from IDEX Health & Science. I have provided a link to more information about the instrument, including an application study, below.

Online Protein Analyzer

First, thank you for participating in our survey. The insights you shared are much appreciated. Second, Yes!, a small, low cost bioprocess controller like the one mentioned in the survey does exist. The link below will take you to an example of the controller being used for fluid management, and provide you with contact details for the manufacturer.

Flotek Controller

Not that long ago such a question would have me reaching for the name of a good machine shop. One capable not only of machining the 316 S.S., complete with ports and requisite threads, but of getting the Ra finish on the wetted surface just right, but not today. Instead, I'm going to recommend an industry leading manufacturer of benchtop bioreactors with decades of experience in the design and manufacture of traditional bioreactor headplates, ports and associated accessories. That just so happens to now have the ability to manufacture fully customizable headplates, ports and the like by utilizing 3D printing technology.

AppiFlex ST

Based on your description, I think it is safe to say you saw the orbital shaken bioreactor system from Kuhner. I too have seen these systems in operation at conferences and the novel way in which they agitate. The system you describe as being benchtop is likely their SB10 which has a working volume of 3L to 12L. I understand there is also an SB50 and SB200 which will take you all the way up to 200L working volume to ensure scalability.

Kuhner SB10

Now this is a classic, from the reference to a 25mm threaded port, to the fact that the port in question is referred to as an 'ingold'. In this case, one classic solution deserves another. I would recommend the Keofitt sampling valve, which is fast approaching its 40th birthday and still going strong with 350,000+ valves rumored to be in operation. Also, since you did not mention if the port on your vessel is the standard or safety design, be sure to double check as this will determine which Keofitt valve length you need for a correct o-ring placement.


You are in luck, gone are the days of your only choice being a 19mm Optical Density (OD) probe. Today, you have no fewer than three (3) from which to choose. The Optek comes in either a 5 or 10mm optical path length (OPL) while the Sartorius offering comes in a 10mm OPL. The Mettler Toledo solution uses backscattering in place of absorbance so no OPL. The one best for you will really depend on the specifics of your application.




Mettler Toledo

Not that long ago I would be telling you to invest in a good pelican case and make plans to ship them to Europe for any type of re-calibration, NIST or otherwise. However, recently Sonotec introduced the ability to conduct calibrations and re-calibrations alike, including NIST and ISO 17025, here in the States for their sensors. If your flow sensors are Sonotec then you should be in good shape. If not, it would not hurt to ask if they might be able to assist you with the sensors you do have.


Thank you for the clarification that this is a microbial fermentation process. I'm going to go ahead and assume that by pilot and manufacturing scale you mean a working volume of 300 liters or greater. Based on those requirements I would recommend you take a look at the offerings from Abec, GE and Thermo Fisher. Each has made significant investments in research and engineering to address the specific challenges associated with microbial fermentation in a single-use platform, such as cooling for instance.



Thermo Fisher

First, let me say upfront that I consider 'outside the box' to be a term of endearment. However, it is a term that can be used to describe just a handful of system integrators in our bioprocessing community. Having said that, based on your location, I'm going to assume you would prefer that the integrator have a strong presence on the West Coast of the U.S. And knowing that your site is a user of the DeltaV platform, I will assume you need them to be proficient in DeltaV. If those two assumptions are true, and 'outside the box' is what you are looking for, then I would suggest you speak to the folks at AES.


I'm going to need a DeLorean for this one and go back in time. There are a number of tools available that I can recommend based on first hand, albeit somewhat dated, experience. They include the Membrane Tester, Polarizer, Dust Caps and Calibration Caps. You might also want to take a look at diatomaceous earth (toothpaste) for cleaning the anode/cathode. Here is a link to the BroadleyJames DO Accessories site for your reference. It appears to have everything I remember, but the toothpaste, and I believe these tools work with Mettler and Hamilton DO probes as well, but be sure to check.

DO Accessories

In recent years there has been a lot of work done to address the need for ultra-low-temperature bioprocessing solutions. One example is the recently introduced AdvantaSil ultra-low-temperature silicone tubing from AdvantaPure which should more than meet your needs. On a related note, if you find yourself looking for a container for this application you would be well served to take a look at the also recently introduced Gore STA-PURE Flexible Freeze Containers.

AdvantaSil Ultra Low Temperature Silicone Tubing

STA-PURE Flexible Freeze Containers

To answer this question effectively I'm going to have to make the assumption that your application is a bioreactor or fermentor of some scale. That being said, I would strongly suggest utilizing the FISP product from Flownamics. It is a proven solution for cell free sampling in bioreactors and fermentors at scales ranging from PD thru Pilot and Large Scale Manufacturing.


Recognizing that your PD scale application will inevitably need the flexibility of scaling-up, and having had first hand positive experiences with many of the folks behind the system, past and present, the Xcell ATF System from Repligen is the way to go. It is truly scalable to your bioprocess, has a proven history of favorable performance and is available in a single-use version if you are interested.

Xcell ATF System

While there are a number of highly qualified proven solutions to the measurement of individual elements of a bioprocess, the ability to monitor multiple elements of a bioprocess continuously in real-time is currently the role of only two core principles; Raman and Near-infrared (NIR). The successful implementation of those principles as products specific to bioprocessing appear below;

Kaiser Optical RamanRxn2 (Raman)

Sartorius Stedim BioPAT Spectro PND500 (NIR)

While for many years the demand upon bioreactor manufacturers was to ensure scalability to larger and larger vessels, that is increasingly less often the case today. Instead, end users are seeking bioreactors, sometimes referred to as "mini-bioreactors", that have design and control features similar to their larger cousins so as to best enable a scaling down of their bioprocess. Here are some industry leading examples;

ambr250 - Sartorius

DASbox - Eppendorf

MiniBio2 - Applikon

While there are many challenges facing our community, among the more immediate have to do with the collection and utilization of data associated with our bioprocesses, and the use thereof for their optimization. The products noted below have been developed to leverage said data to achieve the desired optimization.

Applied Materials SmartFactory RX

Applikon Lucullus PIMS

OSIsoft PI System

Seeq R Series

Bio-G Software Suite

The ongoing adoption of single-use solutions for applications in bioprocessing has led to the debut of not one but five and counting pump solutions that feature single-use wetted components. The pumps below address a wide range of applications in bioprocessing at an assortment of scales.

Watson Marlow Quantum

Quatroflow QF Single-Use

Levitronix PuraLev Single-Use

Cole-Parmer Masterflex QF Single-Use

Quantex Single-Use

Yes. Recently a single-use manifold for fluid transfer was introduced to our community that in itself does not require the use of Y connectors, stopcocks and the like. It fits 3/8" tubing ID's and is capable of a max pressure of 15 psi. No word yet if there are plans to accommodate different tubing ID's or if an automated version is in the works.

Nordson CYLINDRAFlow™ Manifolds

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