Patents

A U.S. Patent for "Method for Using Light Scattering in Real Time to Directly Monitor and Control Impurity Removal in Purification Processes" was awarded to Amgen on November 19th, 2019. The present invention is a methodfor controlling levels of biological impurities in an elution pool from a cation exchange chromatography resin comprising: loading a mixture containing a recombinant protein and biological impurities on to a cation exchange chromatography resin; eluting by a salt gradient the recombinant protein from the cation exchange chromatography resin; measuring the intensity of light scattered by the eluate with a laser light. Learn More

A U.S. Patent Application for "Epigenetic Analysis of Cell Therapy and Related Methods" was awarded to Juno Therapeutics on November 14th, 2019. The present invention is a method of identifying one or more genomic region(s) associated with an outcome of treatment with a cell therapy, the method comprising: (a) analyzing or determining an epigenetic property of one or more genomic regions of a cell or a population of cells, said cell or population comprised in (i) a first composition of cells to be genetically engineered with a recombinant receptor to produce a second composition comprising the recombinant receptor, or (ii) a second composition of cells. Learn More

A U.S. Patent Application for "Methods for Evaluating Monoclonality" was awarded to Lonza on November 14th, 2019. The present invention is a method of evaluating a value for probability of monoclonality, comprising: a) providing a solution comprising a population of cells; b) forming a plurality of aliquots of the solution; c) identifying aliquots having one cell; and d) providing, for aliquots identified as having one cell, a value for the probability that subsequent growth was monoclonal, thereby evaluating a value for probability of monoclonality. Learn More

A U.S. Patent Application for "Methods and Systems for T Cell Expansion" was awarded to Georgia Tech Research on November 14th, 2019. The present invention provides a system for mimicking the secondary lymphoid organs where suspension cells (e.g., T cells) are expanded; methods of expanding, activating, and transfecting the suspension cells in the synthetic microenvironment, and suspension cells produced by such systems and methods. Learn More

A U.S. Patent for "Antigen-Binding Molecule Capable of Binding Two or More Antigen Molecules Repeatedly" was awarded to Chugai on November 12th, 2019. The present invention is a method of removing an antigen from plasma, the method comprising: administering an antibody to an individual in need of having an antigen removed from the individual's plasma, wherein the antibody binds to the antigen through the antigen-binding domain of the antibody and has a KD(pH5.8)/KD(pH7.4) value, defined as the ratio of KD for the antigen at pH 5.8 and KD for the antigen at pH 7.4, of 40 to 1,000, wherein the antibody binds to the antigen in plasma in vivo... Learn More

A U.S. Patent for "RNA Targeting Methods and Compositions" was awarded to the Salk Institute for Biological Studies on November 12th, 2019. The present invention is a method of targeting one or more target RNA molecules, comprising: contacting one or more target RNA molecules with a non-naturally occurring or engineered clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system comprising: at least one Cas13d protein comprising at least 95% sequence identity to SEQ ID NO: 189, or a nucleic acid molecule encoding the at least one Cas13d protein; and at least one CRISPR-Cas system guide RNA (gRNA)... Learn More

A U.S. Patent Application for "Self-Assembling Protein Nanostructures" was awarded to the University of Washington on November 7th, 2019. The present invention is a method, comprising: generating a plurality of representations of a first protein building block using a computing device; generating a plurality of representations of a second protein building block using the computing device, wherein the first protein building block differs from the second protein building block; generating an arrangement of the plurality of representations of the first protein building block and the plurality of representations of the second protein building block... Learn More

A U.S. Patent for "Microbial Production of Steviol Glycosides" was awarded to Manus Bio on November 5th, 2019. The present invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM. Learn More

A U.S. Patent for "Modified Polynucleotides for the Production of Cytoplasmic and Cytoskeletal Proteins" was awarded to Moderna on November 5th, 2019. The present invention relates to compositions including polynucleotides encoding polypeptides which have been chemically modified by replacing the uridines with 1-methyl-pseudouridine to improve one or more of the stability and/or clearance in tissues, receptor uptake and/or kinetics, cellular access by the compositions, engagement with translational machinery, mRNA half-life, translation efficiency, immune evasion, protein production capacity, secretion efficiency, accessibility... Learn More

A U.S. Patent for a "Process and System for Obtaining Botulinum Neurotoxin" was awarded to Allergan on November 5th, 2019. The present invention is a process for producing a biologically active botulinum neurotoxin, the method comprising the following steps: providing a fermentation medium which is free of animal-derived products; fermenting Clostridium botulinum bacteria in the fermentation medium; contacting the fermentation medium with an anion exchange chromatography medium; then contacting an eluate of the anion exchange chrom medium with a cation exchange chromatography medium; and eluting an eluate. Learn More

A U.S. Patent Application for a "Method for Purifying Antibodies Using PBS" was published by the U.S. Department of Health and Human Services on October 31st, 2019. The present invention includes a method for purifying a biologic composition, comprising diafiltering the biologic composition into a composition comprising phosphate buffered saline (PBS) to obtain a purified composition. The method disclosed here can be particularly useful for removing one or more impurities from the biologic composition, such as bis methane (Bis-tris). Learn More

A U.S. Patent for "Preparing Antibodies from CHO Cell Cultures for Conjugation" was awarded to Seattle Genetics on October 29th, 2019. The present invention is based in part on the observation that a CHO cell oxidizing enzyme, particularly QSOX1, can survive a seemingly rigorous antibody purification process to reduce subsequent conjugation efficiency of the antibody to a drug. Whether the oxidizing enzyme survives the purification procedure depends on which purification techniques are employed which can vary from one antibody to another. Learn More

A U.S. Patent for "Efficient Selectivity of Recombinant Proteins" was  awarded to Regeneron  on October 29th, 2019 . The present invention provides a new expression system comprising a mammalian selectable marker that promotes desirable post-translational modifications of glycoproteins. In particular, the invention includes methods and compositions for optimal recombinant protein expression in mammalian cells by employing a selection marker system based on GPT genes of mammalian origin. The invention includes methods that facilitate selectivity and enhanced expression copies as well as protein yield of recombinant proteins in mammalian cells, and methods of using GPT expression systems. Learn More

A U.S. Patent for "Robust Antibody Purification" was awarded to Merck on October 29th, 2019. The present invention is a method for the separation of host cell proteins, antibody fragments and low molecular weight substances from solutions containing antibodies, said method comprising: contacting a solution containing antibodies with a hydrophobic chromatography material for a period of time whereby the antibodies stay in solution and host cell proteins, antibody fragments and low molecular weight substances are adsorbed by the hydrophobic chromatography material... Learn More

A U.S. Patent Application for "Chromatography Column Qualification in Manufacturing Methods for Producing Anti-IL12/IL23 Antibody Compositions" was published by Janssen Biotech on October 24th, 2019. The present invention is a method of operating a chromatography column is described for use in methods of manufacture for producing anti-IL-12/IL-23p40 antibodies, e.g., the anti-IL-12/IL-23p40 antibody STELARA.RTM. (ustekinumab). This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column... Learn More

A U.S. Patent for "Delivery Methods and Compositions for Nuclease-Mediated Genome Engineering" was awarded to Sangamo Therapeutics on October 22nd, 2019. The present invention is a method of introducing a donor nucleic acid molecule into an isolated cell, the method comprising: administering to the cell at least one adeno-associated virus (AAV) vector comprising a donor nucleic acid molecule in the presence of at least one inhibitor of Platelet-derived growth factor receptor under conditions sufficient to increase efficiency of viral delivery to the cell, wherein the AAV vector has an AAV6 capsid. Learn More

A U.S. Patent for a "Method of Using Human FC-Bearing IGG Antibodies to Polyethylene Glycol" was awarded to Bristol-Myers Squibb on October 22nd, 2019.  The present invention is a method of detecting human IgG anti-polyethylene glycol (PEG) antibodies in a sample from a human subject comprising: a. exposing a PEG-coated surface to a sample from a human subject, wherein the sample is blood, serum or any other blood fraction; b. washing the PEG-coated surface; c. detecting the presence or absence of human IgG anti-PEG antibodies bound to the PEG-coated surface; and d. determining the presence... Learn More

A U.S. Patent Application for "A Method of Enhancing the Performance of Chromatography Methods for Purification of Proteins" was published by A*STAR on October 17th, 2019. The present invention describes compositions, kits, and methods for removing contaminants from a protein preparation, for example a cell culture harvest comprising a recombinant protein. The methods described herein can enhance the performance of a later applied chromatographic purification step. The method generally involves contacting a protein preparation comprising a desired protein with a combination of allantoin, a cationic polymer, and a fatty acid... Learn More

A U.S. Patent Application for the "In Vivo Production of Proteins" was published by Moderna on October 17th, 2019. The present invention relates to compositions including polynucleotides encoding polypeptides which have been chemically modified by replacing the uridines with 1-methyl-pseudouridine to improve one or more of the stability and/or clearance in tissues, receptor uptake and/or kinetics, cellular access by the compositions, engagement with translational machinery, mRNA half-life, translation efficiency, immune evasion, protein production capacity, secretion efficiency, accessibility to circulation... Learn More

A U.S. Patent for a "Fermentation Process for Producing Itaconic Acid Under Nitrogen Free Conditions" was awarded to DSM  on October 15th, 2019 . The present invention relates to a method for the production of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid in a suitable fermentation medium, thereby to produce itaconic acid, wherein: (a) the recombinant cell: (i) overexpresses cis-aconitate decarboxylase; and (ii) overexpresses a part of the citric acid cycle and/or has reduced activity of a native metabolic route to acetate and/or lactate; and, optionally, (b) the fermentation is carried out under anaerobic conditions. Learn More

A U.S. Patent for an "Impeller for Bioprocessing" was awarded to Pall  on October 15th, 2019 . The present invention is an impeller for a biocontainer comprising: (a) a hub including a top end and a bottom end, and at least first and second impeller arm slots arranged vertically and extending from the top end toward the bottom end; (b) at least first and second impeller blades extending from the hub, each impeller blade comprising a blade face, an arm having a first end and a second end and a notch between the first end and the second end, wherein the first end is adjacent to the blade face, and the second end extends into an impeller slot... Learn More

A U.S. Patent for a " Heat Transfer Baffle System and Uses Thereof"  was awarded to Abec on October 8th, 2019. The present invention is a reaction vessel comprising in its interior at least one heat transfer baffle, each heat transfer baffle comprising at least one subassembly comprising two distribution channels formed between a first material and a support material, at least one relief channel, and at least one closure bar having an adjustable width that determines the width of the relief channel and that is fixably attached to the support material, the support material further being attached to the reaction vessel at a substantially seamless boundary. Learn More

A U.S. Patent of "System and Method for Production of On-Demand Proteins in a Portable Unit for Point of Care Delivery" was awarded to the University of Maryland on October 8th, 2019.  The present invention is a method of delivering freshly synthesized therapeutic protein exhibiting increased activity and potency to a patient, said method comprising: delivering an on-demand cell-free synthesized therapeutic protein to a patient in need of said therapeutic protein, wherein said therapeutic protein is synthesized using a portable bioprocessing system comprising: a production module for producing the therapeutic protein, and a purification module. Learn More

A U.S. Patent on "Methods for Making Recombinant Proteins" was awarded to Coherus Biosciences on October 8th, 2019.  The present invention is a method of producing adalimumab in a mammalian cell culture comprising: a) inoculating a fed-batch bioreactor containing a cell culture production medium with mammalian cells comprising a nucleic acid encoding adalimumab or a fragment thereof wherein the mammalian cells are at a concentration from about 0.1 million to about 2 million cells/milliliter, wherein the production medium comprises glucose at an initial concentration from about 0.1 to about 0.9 grams/liter... Learn More

A U.S. Patent for "Rupture Disks for Bioreactors and Methods of Using Same" was awarded to Lonza on October 8th, 2019. The present invention is a rupture disk that includes a body including an internal wall extending from a first end of the body to a second end of the body. The internal wall defines an opening extending through the body. In some embodiments, the body further includes an internal flange formed on the internal wall, and the internal flange has a stepped surface. A rupture membrane is positioned within the body, and secured, for example, by cold welding. The rupture membrane may be secured to the stepped surface. Learn More

A U.S. Patent Application for "Control of Cell Growth Through a Temperature Feedback Loop" was published by Bristol-Myers Squibb on October 3rd, 2019. The present invention relates to the use of online biomass capacitance monitoring in cultures as a way to control the growth of cells through the use of a temperature control loop. In certain embodiments, a biomass capacitance probe is used to measure the cell density, and a predetermined growth curve is used to adjust the temperature in the culture. Learn More

A U.S. Patent for "Methods for Transduction and Cell Processing" was awarded to Juno Therapeutics on October 1st, 2019. The present invention is a method, system, and kit for cell processing, e.g., for therapeutic use, such as for adoptive cell therapy. The provided methods include transduction methods, in which cells and virus are incubated under conditions that result in transduction of the cells with a viral vector. The incubation in some embodiments is carried out in an internal cavity of a generally rigid centrifugal chamber, such as a cylindrical chamber made of hard plastic, the cavity of which may have a variable volume. Learn More

A U.S. Patent for a "Mixing Device for Mixing the Contents of a Container" was awarded to Sartorius on October 1st, 2019. The present invention is a mixing device for mixing contents of a container has a support for supporting the container and a moving device connected to the support for moving the support and mixing the contents of the container. The support has a positioning device to position the container so that a removal side of the container is arranged substantially along a predetermined line of the support. The support is pivotable about a removal pivot axis to move the container into a removal position. Learn More

A U.S. Patent for "Automated Multi-Step Purification System" was awarded to GE on October 1st, 2019. The present invention is an automated two step chromatography purification system comprising a, system controller, a capture flow path comprising at least one pump, an elution flow path comprising at least one pump, and a valve arrangement for selective connection of two capture columns to the capture flow path and the elution flow path respectively such that both flow paths may be operated simultaneously and in parallel. Learn More

A U.S. Patent Application for "Packaging for a Flexible Container and Transport Unit" was published by Sartorius Stedim Biotech on September 26th, 2019. The present invention is packaging for a flexible container, in particular for a disposable bag for use in a bioreactor, comprises a bottom part for fixing a first portion of the container, a ceiling part for fixing an opposite second portion of the container, a receiving space which on a first side is defined by the bottom part and on an opposite second side is defined by the ceiling part, and struts arranged between the bottom part and the ceiling part, which fix a defined distance between the bottom part and the ceiling part. Learn More

A U.S. Patent Application for and"Airlift Perfusion Bioreactor for the Culture of Cells" was published by the Keck Graduate Institute on September 26th, 2019. The present invention is a bioreactor apparatus, comprising: a cell culture zone comprising a riser section and a downcomer section; a settling zone comprising an inclined gravity settler in fluid communication with the downcomer section, the inclined gravity settler being partitioned from the riser section; a gas injection inlet to the riser section; a liquid inlet to the cell culture zone; and a liquid outlet on the inclined gravity settler. Learn More

A U.S. Patent for "Method and System for Leak Testing" was awarded to Pall on September 24th, 2019. The present invention is a method of leak testing a flexible expandable bag having at least one port and at least one flexible wall in a system comprising: a gas flow meter; a gas pressure regulator; a gas pressure transducer; a gas pressure relief valve; a displacement measurement sensor, arranged to measure displacement of the flexible wall of the bag as the bag expands while being filled with gas; at least three valves, each valve being controllable to allow or prevent gas flow therethrough. Learn More

A U.S. Patent for "Microcarrier Perfusion Culturing Methods and Uses Thereof" was published by Genzyme on September 24th, 2019. The present invention is a method of culturing an adherent Chinese hamster ovary (CHO) cell, the method comprising: providing a conical shake tube containing an adherent CHO cell disposed in a first liquid culture medium, wherein the first liquid culture medium occupies about 10% to about 30% of the volume of the conical shake tube and contains a plurality of microcarriers at a concentration of about 1.0 g/L to about 15.0 g/L, wherein the microcarriers have (1) an average diameter of about 200 Tm to about 300 Tm. Learn More

A U.S. Patent Application for "Culture Media for Culturing Pluripotent Stem Cells in Suspension" was published by Accellta on September 19th, 2019. The present invention is defined culture media for culturing pluripotent stem cells in a suspension culture devoid of substrate adherence, the defined culture media comprising an effective amount of a protease inhibitor; a GSK3.beta. inhibitor and at least one agent selected from the group consisting of a protease inhibitor and a WNT3A polypeptide; a WNT3A polypeptide and a stabilizing agent thereof with the proviso that said stabilizing agent is not a lipid vesicle; and/or a GSK3.beta. inhibitor, with the proviso that the medium is devoid of an ERK1/2 inhibitor. LEARN MORE

A U.S. Patent Application for the "Detection of pH Utilizing Raman" was published by UK Research & Innovation on September 12th, 2019. The present invention includes methods and apparatus for measuring pH in a sub-surface volume of a diffusely scattering sample. Probe light is directed to an entry region on the sample surface, and collected from a collection region on the sample surface following diffuse scattering within the sample. The collection region is spatially offset from the entry region, so that when one or more Raman spectral features are detected in the collected probe light, a pH of the sub-surface volume can be determined from the spectral features. Learn More

A U.S. Patent Application for "High Efficiency Continuous Countercurrent Tangential Negative Chromatography" was published by Chromatan on September 12th, 2019. The present invention is a system, module and method for continuous or batch single-pass countercurrent tangential chromatography for bind/elute and negative chromatography applications. The system includes binding, washing, elution (for bind/elute), regeneration, and equilibration single-pass modules. The resin slurry flows in a continuous single pass at steady-state through each module, while corresponding buffers flow countercurrent to the slurry facilitating efficient product and impurity extraction. Learn More

A U.S. Patent Application for a "Chromatography Medium" was published by Puridify on September 12th, 2019. The present invention is a chromatography medium comprising one or more electrospun polymer nanofibres wherein the electrospun cellulose nanofibers are fused together at points where the nanofibers intersect one another, and which in use form a stationary phase comprising a plurality of pores through which a mobile phase can permeate, and wherein the chromatography medium has affinity chromatography protein ligand surface functionality interactive with target molecules. Learn More

A U.S. Patent for a "Particle Sensor with Interferent Discrimination" was awarded to BugLab on September 10th, 2019. The present invention provides methods and devices to measure particle suspension concentrations in the presence of potential interferents. Particle back-scatter readings are taken at light wavelengths that are absorbed by the medium before interacting with surrounding objects. Source-detector spacings are minimized compared to the mean absorbance path length of light, thereby maximizing the range of sensitivity to particle concentration. The active discrimination against potentially interfering particles, such as bubbles, is provided. Learn More

A U.S. Patent for a "Reagent Usable for the Isolation and/or Purification of Nucleic Acids" was awarded to Qiagen on September 10th, 2019. The present invention provides a special reagent for the pretreatment of sample materials. The pretreatment with the reagent according to the invention enables the isolation of nucleic acids by a uniform method from very diverse sample materials, in particular different bioprocess samples, even if the nucleic acids are only present in small amounts in the sample materials. The reagent used for the pretreatment comprises, as key component, at least one compound comprising an amino group. Learn More

A U.S. Patent Application for a "Signal Polypeptide for Improved Secretion of Protein" was published by MedImmune on September 5th, 2019. The present invention relates to a signal polypeptide for improving excretory production of a heterologous polypeptide, proteins comprising the signal polypeptide, nucleic acids encoding the signal polypeptide, and methods of producing thereof. Exemplary embodiments include an improved signal polypeptide attached to alpha toxin, and methods to produce and isolate alpha toxin. Learn More

A U.S. Patent Application for "Multivariate Spectral Analysis and Monitoring for Biomanufacturing" was published by Genzyme on September 5th, 2019. The present invention features methods that include obtaining a vibrational spectrum of a solution in a biological manufacturing system, analyzing the vibrational spectrum using a first chemometrics model to determine a value of a first quality attribute associated with the solution, analyzing the vibrational spectrum using a second chemometrics model to determine a value of a second quality attribute associated with the solution, and adjusting at least one parameter of a purification unit. Learn More

A U.S. Patent Application for "Chromatography Column Packing Medium Recovery" was published by Repligen on September 5th, 2019. The present invention includes a chromatography column tube having an inlet and outlet and port assembly arranged in a wall of the column tube between flow distributors that together form a chamber within the tube that is filled with packing medium are described. The port assembly facilitates the removal of resin from the pre-packed column and the port assembly does not affect fluid flow in the normal use of the column for chromatographic separation or ability to maintain sanitary conditions within the column. Learn More

A U.S. Patent for "Cell-Sorting Method and Associated Device" was awarded to Commissariat a L'Energie Atomique on September 3rd, 2019. The present invention is a method of discriminating a living cell from a dead cell, and a device for implementing the method, the method including: using a lens-free imaging device to acquire a diffraction figure corresponding to a cell; and determining a light intensity on a central area of an elementary diffraction figure associated with the cell. It can thus be determined if the studied cell is a living cell or a dead cell. Learn More

A U.S. Patent for "Sequencing Methods" was awarded to 10K Genomics on August 27th, 2019. The present invention described herein solves challenges in providing a proficient, rapid and meaningful analysis of sequencing data. Methods and computer program products of the invention allow for a system to receive, analyze, and display sequencing data in real-time. The invention provides solutions to several difficulties encountered in assembling short sequencing-reads, and by doing so the invention functions to improve the worth and significance of sequencing data. Learn More

A U.S. Patent for a "Method of Viral Purification" was awarded to GlaxoSmithKline  on August 27th, 2019. The present invention is a method of producing a virus in cell culture comprising at least the steps of: a) providing a population of cells in a cell culture medium, b) infecting the population of cells by: i. inoculating the population of cells with the virus, and ii. incubating the inoculated population of cells so as to allow the virus to replicate and propagate, c) collecting the produced virus, thereby providing a viral harvest, and d) purifying the virus, wherein a volumetric power input of at least 15... Learn More

A U.S. Patent Application for a "Process for Improving the Dissolution Behavior of Components in Aqueous Solutions" was published by MilliporeSigma on August 22nd, 2019. The present invention relates to a process for improving the solubility or dissolution behaviour of components in an aqueous solution. The dissolution properties of a slowly/badly dissolving component can be positively affected by generating mixed particles of this component with another component which has a negative dissolution enthalpy. By preparing and adding such mixed particles solubility is improved without changing the chemical composition. Learn More

A U.S. Patent Application for a "Virtual Transmitter for Bioreactor Automation System" was published by Finesse on August 22nd, 2019. The present invention includes a probe for measuring a condition in a bioreactor that can generate a signal. Measured conditions may include pH, DO, temperature, and pressure. The signal can travel to an electronic card capable of converting the signal from analog to digital and the card can then send the converted signal to a digital controller. The condition monitoring and control system eliminates hardware redundancies and increases versatility by then displaying the converted signal in a software-based virtual transmitter on a non-dedicated human machine interface. Learn More

A U.S. Patent on "Media for Culturing Stem Cells" was awarded to the Technion Research & Development Foundation on August 20th, 2019. The present invention is a well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of maintaining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. Learn More

A U.S. Patent for a "Process of Recycling Mixed PET Plastic Articles" was awarded to Carbios  on August 20th, 2019. The present invention relates to a method for recycling at least one mixed PET plastic article, the method comprising degrading at least PET of the mixed PET plastic article to monomers and/or oligomers using an enzyme and recovering the resulting monomers and/or oligomers. The method of the invention may be used for depolymerizing, simultaneously or sequentially at least two different polymers of the mixed PET plastic article, and/or for recycling at least two mixed PET plastic articles. Learn More

A U.S. Patent Application for an "Apparatus for Detecting pH and Dissolved Oxygen" was published by Scientific Industries  on August 15th, 2019 . The present invention is a cell culture monitoring system that may include a device having collimating and filtering components to elicit and detect fluorescence from a substrate located within a reaction vessel. The device may be used to detect changes in pH and dissolved oxygen levels in a liquid contained in the reaction vessel due to growth of living cells. Excitation light beams can be generated and collimated by a beam combiner and directed into the reaction vessel so as to be incident upon the substrate to cause the substrate to fluoresce. Learn More

A U.S. Patent for "Highly Concentrated Pharmaceutical Formulations" was awarded to Genentech  on August 13th, 2019 . The present invention relates to a highly concentrated, stable pharmaceutical formulation of a pharmaceutically active anti-CD20 antibody, such as e.g. Rituximab, Ocrelizumab, or HuMab<CD20>, or a mixture of such antibody molecules for subcutaneous injection. In particular, the present invention relates to formulations comprising, in addition to a suitable amount of the anti-CD20 antibody, an effective amount of at least one hyaluronidase enzyme as a combined formulation or for use in form of a co-formulation. Learn More

A U.S. Patent for "Methods for Suspension Cultures of Human Pluripotent Stem Cells" was awarded to Janssen on August 13th, 2019. The present invention is an in vitro method of producing three-dimensional cell clusters of pluripotent stem cells comprising the steps of: a. treating pluripotent stem cells cultured in a planar adherent culture with a chelating agent or enzyme to release cell aggregates from the planar adherent culture; b. suspending the cell aggregates from the planar adherent culture in a culture medium in the presence of a Rho-kinase inhibitor without centrifuging the cell aggregates and without dissociating the cell aggregates to single cells. Learn More

A U.S. Patent Application for a "Reversible Liquid Filtration System" was published by Sartorius Stedim on August 8th, 2019. The present invention is a reversible liquid filtration system for cell culture perfusion comprises: a bioreactor vessel (B4), for storing the cell culture (L4); a perfusion pump (P7), comprising a reciprocable element (P71) which is movable in opposing first and second pumping directions (dF, dR); a filter (F4); and first and second bi-directional valves (BV1, BV2), each selectively controllable between open and closed positions. The perfusion pump (P7), the filter (F4), and the first and second bi-directional valves...  Learn More

A U.S. Patent Application for a "Chromatography Method" was published by GE on August 8th, 2019. The present invention is a method of making affinity chromatography beads suitable for purification of immunoglobulin containing proteins or parts thereof, from contaminants that are larger than immunoglobulin containing proteins, the method comprising: a) providing allylated chromatography beads having an allylated shell layer and an allylated inner core wrapped around by the allylated shell layer; b) inactivating the allylated shell layer of the beads to form a lid of hydrolyzed allyl groups having a thickness of 1-8 .mu.m; c) activating the allylated inner core of the beads. Learn More

A U.S. Patent for "Reusable Aseptic Fluid Couplings" was awarded to Colder Products Company on August 6th, 2019. The present invention is an aseptic fluid coupling system comprising: a first coupling portion comprising: a first valve body defining a first longitudinal axis and a first end port; a first valve member disposed within the first valve body; and a cap that is releasably coupleable to the first valve body, the cap enclosing at least a portion of the first valve body while the cap is coupled to the first valve body; and a second coupling portion configured to releasably couple with the first coupling portion, comprising...  Learn More

A U.S. Patent for "Variable Diameter Bioreactors" was awarded to Lonza on August 6th, 2019. The present invention is a variable diameter bioreactor vessel that can include a first vessel section having a first diameter configured to hold a liquid media and biologic material and a second vessel section having a second diameter that is greater than the first diameter such that the liquid media can be increased from a first volume to a second volume within the vessel. In some aspects, the first vessel section can have an aspect ratio of greater than 0.3:1. In some aspects, the second vessel section can have an aspect ratio of greater than 0.3:1. Learn More

A U.S. Patent Application for "Binding Proteins, Including Antibodies, Antibody Derivatives and Antibody Fragments, that Specifically Bind CD154 and Uses Thereof" was published by UCB on August 1st, 2019. The present invention provides binding proteins, including antibodies, antibody derivatives and antibody fragments, that specifically bind a CD154 (CD40L) protein. This invention also provides a chimeric, humanized or fully human antibody, antibody derivative or antibody fragment that specifically binds to an epitope to which a humanized Fab fragment comprising a variable heavy chain sequence according to SEQ ID NO... Learn More

A U.S. Patent Application for a "Method of Culturing Eukaryotic Cells" was published by Genentech on August 1st, 2019. The present invention is an apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO.sub.2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range. Learn More

A U.S. Patent for "Large Scale Disposable Bioreactor" was awarded to Protalix on July 30th, 2019. The present invention is a reusable, disposable device for culturing plant tissues or cells including a non-rigid container having dimensions and gas exchange ports designed for maintaining oxygen saturation and shear forces suitable for culturing plant tissue or cells in 400 liters or more of culture medium is provided. Also provided are methods for producing a catalytically active human recombinant protein in a plant cell, using the disposable device of one of the embodiments of the instant specification. Learn More

A U.S. Patent for "Ion Exchange Membrane Chromatography" was awarded to Genentech on July 30th, 2019. The present invention are methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength... Learn More

A U.S. Patent Application for a "Method for Packing Chromatography Columns" was published by GE on July 25th, 2019. The present invention discloses a method for packing a plurality of uniform chromatography columns, comprising the steps of: a) providing a plurality of chromatography columns; b) providing a plurality of chromatography resin aliquots; c) packing the chromatography resin aliquots in the chromatography columns to provide a plurality of packed chromatography columns; and d) subjecting the packed chromatography columns to repeated mechanical impacts to provide a plurality of uniform chromatography columns. Learn More

A U.S. Patent Application for "Cell Culture Improvements" was published by AbbVie on July 25th, 2019. The present invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture. Learn More

A U.S. Patent Application for a "Computer Implemented Method for Creating a Fermentation Model" was published by Bayer on July 25th, 2019. The present invention is a computer-implemented method for model-based estimation of the state of a bioreaction with an organism in a bioreactor comprising: creating a model of a bioreaction with an organism, said model including a matrix L, kinetics of macroreactions and model parameters of calculated values, which comprises the following steps: a. defining selected metabolic pathways of the organism, their properties of stoichiometry and reversibility as background knowledge and calculating... Learn More

A U.S. Patent for a "Dual Wavelength Raman Probe with Spectral Concatenation of Data Filtration Systems" was awarded to Innovative Phototonic Solutions on July 23rd, 2019. The present invention is a compact dual-wavelength Raman probe using two laser sources each providing Raman excitation light at a different wavelength is disclosed causing Raman scattering in a fingerprint region associated with one excitation wavelength and causing Raman scattering in a stretch region, which are detected by the same detector array. Learn More

A U.S. Patent for "Perfusion Filtration Systems" was awarded to Artemis Biosystems on July 23rd, 2019. The present invention is a method of filtering a cell culture media comprising passing a cell culture media comprising mammalian cells and a virus through a plurality of tubular porous membranes of a filter elements enclosed within a casing, wherein each tubular porous membrane filter element comprises a plurality of sintered tubular ultra-high-molecular-weight polyethylene (UHMWPE) porous membranes, wherein at least 95% of the pores in each of the plurality of tubular porous membranes are between 5 .mu.m and -15 .mu.m in size... Learn More

A U.S. Patent Application for an "Aseptic Bioreactor Sampling System" was published by Trizell on July 18th, 2019. The present invention is an adherent cell culture bioreactor comprising: a container having an interior for containing liquid cell culture media and a solid support for adherent cell culture, the container interior in flowable communication with a first tube and a second tube, each of said tubes comprising an intake end in flowable communication with the container interior, and an output flush end and an output sample end, whereby liquid cell culture media can flow from the container interior into the intake end of the tube... Learn More

A U.S. Patent Application for "A Process for Degrading Plastic Products" was published by Carbios on July 18th, 2019. The present invention relates to processes for degrading plastic products and the uses thereof. The processes of the invention particularly comprise a step of amorphizing a plastic product prior to a step of depolymerization. The processes of the invention are particularly useful for degrading a plastic product comprising polyethylene terephthalate and/or polylactic acid. The invention also relates to a method of producing monomers and/or oligomers from a plastic product comprising at least one polyester... Learn More

A U.S. Patent for "Pharmaceutical Glass Packaging Assuring Pharmaceutical Sterility" was awarded to Corning on July 16th, 2019.  The present invention is a sterile glass pharmaceutical container or vessel such as, but not limited to, vials for holding pharmaceutical products or vaccines in a hermetic and/or sterile state. The sterile glass pharmaceutical container undergoes a strengthening process that produces compression at the surface and tension within the container wall. The strengthening process is designed such that the tension within the wall is great enough to ensure catastrophic failure of the pharmaceutical container, thus rendering the product unusable... Learn More

A U.S. Patent for "Apparatus and Methods for Aseptic Fluid Interconnects" was awarded to ERBI Biosystems on July 16th, 2019.  The present invention provides apparatus and methods for making multiple aseptic fluid interconnections in parallel with an integral clamping mechanism and elastomeric fluid sealing gasket to provide an initial fluid tight seal while removing sealing tape tabs and a final fluid tight seal after sealing tape tabs are removed. The clamping mechanism comprises a hinge coupling and a first set of snap-fit locks to maintain the initial fluid tight seal and a second set of snap-fit locks to maintain the final fluid tight seal. Learn More

A U.S. Patent for "Processes for Filtering Liquids Using Single Pass Tangential Flow Filtration Systems and Tangential Flow Filtration Systems with Recirculation of Retentate" was awarded to MilliporeSigma on July 16th, 2019. The present invention is a method of filtering a liquid feed, comprising: a) passing a liquid feed through a single pass tangential flow filtration (SPTFF) system that comprises: a plurality of stacked filtration modules that are fluidly connected, each filtration module comprising: a single manifold segment comprising a first manifold for receiving and carrying the feed into the filtration module, a second manifold for receiving and carrying retentate. Learn More 

A U.S. Patent Application for "Perfusion Bioreactor Bag Assemblies" was published by Juno Therapeutics on July 11th, 2019.  The present invention is a bioreactor bag assembly that can minimize the amount of additional connections/adaptations made to the bioreactor bag before the bioreactor bag can be used for cell cultivation, thereby reducing the risk of contamination. The bioreactor bag assemblies disclosed herein can include a pre-assembled waste bag connection and pre-assembled tubing arrangements so that the cell media and/or the cell source can be immediately welded to the pre-assembled tubing arrangements. Learn More

A U.S. Patent for a "Horizontally Rocked Bioreactor System" was awarded to Bioreactor Sciences on July 9th, 2019. The present invention is a bioreactor system, comprising: a vessel assembly comprising: a tube positioned to lie along a longitudinal axis, the tube having ends; a baffle extending within the tube, the baffle extending along and contacting an inner wall of the tube along the longitudinal axis and extending to and contacting the ends of the tube, wherein a ratio of a major surface area of the baffle to a central cross-sectional area of the tube along the longitudinal axis is greater than about 0.45 and less than about 0.90...  Learn More

A U.S. Patent for a "Magnetophoresis System for Separation of Biological Particles" was awarded to Pablo Sanchez on July 9th, 2019.  The present invention is an apparatus for analyzing magnetically functionalized particles in a sample comprising: a chamber containing the magnetically functionalized particles in the sample; an analytic instrument communicating with the chamber to receive the magnetically functionalized particles in the sample for analysis; a magnetizable collection structure within the chamber for collecting magnetically functionalized particles from the sample.  Learn More

A U.S. Patent for "Systems and Methods for Adaptive Non-Linear Control of Process Systems" was awarded to Kyle Hunte on July 9th, 2019.  The present invention provides systems and methods for generating an adaptive nonlinear controller and utilizing the adaptive nonlinear controller to regulate the operation of nonlinear process systems. In particular, a method is provided for generating a control model by defining an objective function utilizing a target function that specifies the desired response of the system and a state-space model representing the dynamics of the non-linear system. Learn More

A U.S. Patent for a "Process for Reducing Subvisible Particles in a Pharmaceutical Formulation" was awarded to Regeneron on July 9th, 2019. The present invention provides a stable protein composition containing a surfactant and having less than 400 subvisible particles of 10 microns or greater diameter per container, or less than 10,000 subvisible particles of 2 microns or greater per container. A method of manufacturing such a stable protein composition is disclosed, which includes a unit of operation that removes or decreases an esterase activity that degrades the surfactant. The unit of operation may be hydrophobic interaction chromatography or filtration, mixed mode chromatography, or the like. Learn More

A U.S. Patent Application for an "Air Purification System" was published by AWE Solutions on July 4th, 2019.  The present invention is a system and process for purifying air. The system includes a housing and a plurality of inner and outer chambers. A solution of water and a biological reagent is configured to flow around the inner and outer walls of the inner and outer chambers while air is passed adjacent to this fluid flow. This causes an interaction between the air and the fluid solution to cleanse the air. The air is then discharged from the chamber. Learn More

A U.S. Patent Application for "Protein Purification"  was published by UCB on July 4th, 2019.  The present invention relates to a process for purification of a protein comprising a semi-continuous chromatography step whereby the flow-through is collected and re-loaded onto the chromatography matrix. Comprising the steps of a) in an operational chromatography cycle loading a first volume of a mixture containing the protein of interest from a first container to a chromatography matrix operated such that the protein binds to the chromatography matrix until 40% to 100% of the static binding capacity of the chromatography matrix is reached... Learn More

A U.S. Patent for "Processes for Starting Up and Operating Deep Tank Anaerobic Fermentation Reactors for Making Oxygenated Organic Compound from Carbon Monoxide and Hydrogen" was awarded to Synata on July 2nd, 2019. The present invention is a process for starting up and operating anaerobic, deep tank fermentation reactors including a process for anaerobic bioconversion of a gas substrate comprising carbon monoxide, hydrogen, and carbon dioxide in a reactor by contact of the gas substrate with an aqueous menstruum containing microorganisms suitable for converting the gas substrate to an oxygenated organic compound in the reactor. Learn More

A U.S. Patent Application for "Antibody Drug Conjugates" was published by Novartis on June 27th, 2019. The present invention discloses anti-P-cadherin antibodies, antigen binding fragments thereof, and antibody drug conjugates of said antibodies or antigen binding fragments, particularly antibody drug conjugates comprising anti-P-cadherin antibodies conjugated to auristatin analogs. The invention also relates to methods of treating cancer using the antibody drug conjugates. Also disclosed herein are methods of making the antibodies, antigen binding fragments, and antibody drug conjugates, and methods of using the antibodies and antigen binding fragments as diagnostic reagents. Learn More

A U.S. Patent Application for a "Method for Producing Recombinant Protein" was published by Spiber on June 27th, 2019. The present invention relates to a method for production of a recombinant protein using recombinant cells that express the recombinant protein under the control of an inducible promoter, the method including continuous culturing with addition of fresh culture medium to a portion of the culture solution in which the recombinant cells have been grown, wherein the recombinant cells whose expression of the recombinant protein has been induced are not reused. Learn More

A U.S. Patent Application for a "Method for Purification of Viruses or Virus Like Particles Using a Crosslinked Cellulose Hydrate Membrane" was published by Sartorius on June 27th, 2019. The present invention is a method for purifying viruses or virus-like particles from a solution, comprising the following steps: a) providing a mixture M composed of a solution A, which contains the viruses or the virus-like particles to be purified and impurities, and a solution B, which contains a polyalkylene glycol having two or three carbon atoms in the repeat unit, d) loading a crosslinked cellulose hydrate membrane with the mixture M from step... LEARN MORE

A U.S. Patent for a "Solventless Extraction Process" was awarded to DSM on June 25th, 2019. The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from micro-organisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained. Learn More

A U.S. Patent for "Conditionally Active Biological Proteins" was awarded to BioAtla on June 25th, 2019. The present invention is a method of preparing a conditionally active biological protein, the method comprising steps of: i) evolving the DNA which encodes a parent biological protein using one or more evolutionary techniques to create mutant DNAs; ii) expressing the mutant DNAs to obtain mutant biological proteins; iii) subjecting the mutant biological proteins to an assay under a first physiological condition selected from physiological conditions of a first location selected from the group. Learn More

A U.S. Patent Application for "Wireless Sensor Information Monitoring" was published by Lonza on June 20th, 2019. The present invention is a system, apparatus, and method for continuously monitoring data output from a wireless sensor on a mesh network at a frequency higher than mesh formation/propagation. For example, the system may include a wireless gateway, an amplifier coupled to the wireless gateway, a first antenna coupled to the amplifier, a second antenna coupled to the amplifier, and a wireless sensor. The wireless sensor may be a wireless temperature sensor configured to monitor the temperature of a cryopreserved material product. Learn More

A U.S. Patent Application for "Packed Bed Emulsification" was published by GE on June 20th, 2019. The present invention is a system for the production of polysaccharide droplets from a water-in-oil emulsion comprising, at least one chamber comprising an inlet side connectable to a source of water-in-oil emulsion containing droplets of an aqueous solution of a polysaccharide in an oil-based continuous phase, an outlet side, a packed bed of beads between the inlet side and the outlet side, and flow-producing means for providing a flow of water-in-oil emulsion through the packed bed to an outlet port, wherein the beads are hydrophilic beads. Learn More

A U.S. Patent for an "Apparatus, Systems and Methods for Growing Microorganisms" was awarded to Locus on June 18th, 2019. The present invention is a fermentation system that includes a fermentation tank, a plurality of sensors operable to measure a plurality of fermentation system parameters, a plurality of mechanisms operable to control the fermentation system parameters, and a controller in electronic communication with the sensors and with the mechanisms. The controller includes a memory, a receiver circuit, a transmitting circuit, and a control circuit. The memory stores a plurality of recipes each defining user operating instructions and parameter settings. Learn More

A U.S. Patent for "High Efficiency Continuous Countercurrent Tangential Chromatography" was awarded to Chromatan on June 18th, 2019. The present invention is a system, module and method for continuous or batch single-pass countercurrent tangential chromatography for bind/elute and negative chromatography applications. The system includes binding, washing, elution (for bind/elute), regeneration, and equilibration single-pass modules. The resin slurry flows in a continuous single pass at steady-state through each module, while corresponding buffers flow countercurrent to the slurry facilitating efficient product and impurity extraction... Learn More

A U.S. Patent Application for "Cell Culture Additives and Their Use for Increased Bioprotein Production from Cells" was published by Air Liquide on June 13th, 2019. The present invention relates to a method of increasing protein biomolecule production in which a) a cell that produces a heterologous protein biomolecule is cultured, and b) an Additive and/or a source of the Additive is added to the culture medium in an amount sufficient to (i) increase a total yield of the heterologous protein biomolecule secreted into the cell culture media and/or (ii) increase a specific cellular productivity of the heterologous protein biomolecule secreted into the cell culture media. Learn More

A U.S. Patent Application for a "Method of Producing a Recombinant Protein" was published by GSK on June 13th, 2019. The present invention relates to a recombinant signal sequence, and a fusion protein comprising the signal sequence and a recombinant protein. Methods of producing the recombinant protein using the recombinant signal sequence are described. In particular, the recombinant signal sequence provides a method for controlling the viscosity of the fermentation, and/or controlling basal (pre-induction) expression of the recombinant protein. Learn More

A U.S. Patent for a "Method for Producing a Product in a Continuous Cell Culture Fermentation Process"  was awarded to AGC Biologics on June 11th, 2019. The present invention is a method of producing a product selected from a biopolymer expressed by a cell, an intracellular or extracellular product produced by a cell or microorganism, a periplasmatic product produced by a cell or microorganism, a cell or a microorganism in a bioreactor in a chemostat fermentation process, wherein said bioreactor comprises: i) a first outlet having a separation device allowing impurities with a size below the size of the product, and medium to be removed... Learn More

A U.S. Patent Application for "End-to-End Cell Therapy Automation" was published by Lonza on June 6th, 2019. The present invention is a method for automated production of a genetically modified immune cell culture, the method comprising: (a) activating an immune cell culture with an activation reagent to produce an activated immune cell culture; (b) transducing the activated immune cell culture with a vector, to produce a transduced immune cell culture; (c) expanding the transduced immune cell culture; (d) concentrating the expanded immune cell culture of (c); and (e) harvesting the concentrated immune cell culture of (d)... Learn More

A U.S. Patent Application for a "Cell Retention Device and Method" was published by Boehringer Ingelheim on June 6th, 2019. The present invention relates to a cell retention device and method for use in a perfusion cell culture system where the cell retention device includes a hollow-fiber filter having an average pore size ranging from about 0.5 to about 20 .mu.m and having the ability to operate under perfusion cell culture conditions for up to 35 days without being clogged or losing its product sieving ability by more than 20%. Learn More

A U.S. Patent for "Aseptic Coupling Devices" was awarded to Colder Products Company on June 4th, 2019. The present invention is an aseptic coupling arrangement includes a first coupling device and a second aseptic coupling device. In one example, the first and second coupling devices are substantially similar, each having a main body having a front face and a fluid passageway therethrough. A first connecting feature on the main body of each coupling device may be provided for aligning and coupling the aseptic devices together. Each coupling device may also include a sealing member received in the main body and a membrane... Learn More

A U.S. Patent for "Filter Systems for Separating Microcarriers from Cell Culture Solutions" was awarded to Thermo Fisher on May 28th , 2019. The present invention is a filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber... Learn More

A U.S. Patent for a "Regulatable Promoter" was awarded to Lonza on May 28th, 2019. The present invention is a method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising a series of steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate or at least 15% as compared to the native pGAP promoter... Learn More

A U.S. Patent for a "Method of Removing DNA from Biotechnological Products" was awarded to BASF on May 28th, 2019. The present invention relates to a method for degrading DNA in a sample obtained by microbial fermentation or biotransformation, comprising treating the sample with a combination of increased temperature and low pH. It also relates to a method for releasing DNA from a microbial cell, comprising incubating the microbial cell at a temperature of 45.degree. C. to 55.degree. C. for two to ten hours. Finally, the present invention provides a method for producing a product, comprising a step of releasing DNA from a microbial cell and degrading said DNA. Learn More

A U.S. Patent for a "High-Cell Density Fed-Batch Fermentation Process for Producing Recombinant Protein" was awarded to Pfizer on May 28th, 2019. The present invention includes methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions for use in the methods of the present invention, are provided. Learn More

A U.S. Patent Application for an "Automated Bioprocess Development" was awarded to TAP on May 23rd, 2019. The present invention is a method for automating process development in a bioprocessing environment. The method comprising: executing a first experiment run according to a set of parameters; retrieving a first real-time set of data of the experiment nm while the experiment run is being executed; retrieving a second real-time set of data of an experiment run being executed in parallel, analysing the retrieved first real-time set of data and the second real-time set of data to determine an adjusted set of parameters; and, modifying, based on the analysis... Learn More

A U.S. Patent for a "Bioreactor with Condenser" was awarded to Sartorius Stedim on May 21st, 2019. The present invention is a bioreactor with a vessel having at least one gas dissipation duct for gas discharge, the orifice of the gas dissipation duct being connected to a hydrophobic sterile filter and to a condenser arranged between them and having condensation surfaces, a turbulence generator for generating a turbulent flow is arranged in the gas dissipation duct in the region of the condenser. Learn More