Patents

A U.S. Patent Application for "Suspension Culture of Human Embryonic Stem Cells" was published by Asterias Biotherapeutics on January 17th, 2019. The present invention is a method for culturing undifferentiated human pluripotent stem (hPS) cells in suspension, the method comprising: a) suspending undifferentiated hPS cells in a cell culture medium comprising basic fibroblast growth factor (bFGF); b) maintaining the hPS cells substantially undifferentiated while culturing in suspension; c) changing the cell culture medium periodically; and d) optionally splitting the culture from time to time, so as to reduce the density of the hPS cells in culture; wherein the hPS cells are not grown attached to a solid substrate. Learn More

A U.S. Patent for "Methods and Compositions for Increased Safety of Stem Cell-Derived Populations" was awarded to
MIT on January 15th, 2019. The present invention is a method for reducing residual pluripotent stem cells in an in vitro differentiated cell population, comprising culturing an in vitro differentiated cell population at an oxygen partial pressure that is less than 142 mmHg for at least 1 day and less than 10 days, and harvesting the differentiated cell population after the culturing step; wherein the harvested cell population comprises less than 1 pluripotent stem cell per 10.sup.3 cells, wherein 50% or more of the cells in the in vitro differentiated cell population are positive for at least one differentiation marker before the culturing step. Learn More

A U.S. Patent for a "Bioreactor for the Cultivation of Mammalian Cells" was awarded to Lonza on January 15th, 2019. The present invention is a scalable bioreactor for the cultivation of mammalian cells, the bioreactor comprising: a biocompatible tank or vessel having a volume of between 500 liters and 20,000 liters and at least one top impeller and at least one bottom impeller, wherein the at least one top impeller has a power number between 0.1 and 0.9, wherein the at least one top impeller has a flow number between 0.4 and 0.9, wherein the at least one bottom impeller has a power number between 0.5 and 0.9, wherein an impeller spacing between the at least one top impeller. Learn More

A U.S. Patent Application for "Methods for Purification of Recombinant AAV Vectors" was published by Genzyme on
January 10th, 2019. The present invention is a method for isolating a population of recombinant adeno-associated virus (rAAV) particles from in-process impurities in a feedstream, comprising the steps of: (a) (i) contacting a feedstream containing the rAAV particles with an apatite chromatography medium in the presence of polyethylene glycol (PEG), wherein the rAAV particles bind to the apatite chromatography medium; and (ii) eluting the rAAV particles bound to the apatite chromatography medium with an elution buffer... Learn More

A U.S. Patent Application for "Overexpression of N-Glycosylation Pathway Regulators to Modulate Glycosylation of Recombinant Proteins" was published by Amgen on
January 10th, 2019. The present invention is a method of regulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture process, comprising: transfecting a mammalian host cell to overexpress a protein that is involved in an N-glycosylation pathway, wherein the protein involved in the N-glycosylation pathway is selected from the group consisting of N-acetyl-glucosaminyltransferase-1 (encoded by Mgat1); N-acetyl-glucosaminyltransferase-2 (encoded by Mgat2); a UDP-Galactose transporter encoded by Slc35a2... Learn More

A U.S. Patent for "Use of Vibrational Spectroscopy for DNA Content Inspection" was awarded to 1087 Systems on
January 8th, 2019. The present invention is a cytometry system, comprising: a microfluidic channel with a measurement volume in which a cell in a sample fluid is introduced; at least one laser source configured to emit at least one light at one or more wavelengths towards the cell resulting in a detectable change in the light; wherein the at least one laser source is further configured to emit at a wavelength corresponding to the resonant absorption for the cell DNA... Learn More

A U.S. Patent for "Performance Enhancing Genetic Variants of E. coli" was awarded to the Regents of the University of California on January 8th, 2019. The present invention provides mutant Escherichia coli cells that contain one or more mutations in one or more of the rpoB, hns/tdk, corA, ygaZ, iap, metL, ygeW, and pyrE/rph genes (exemplified in Table 2A and 2B), which confer on the mutant in minimal media the phenotype of increased level of growth and/or increased glucose uptake rate and/or increased acetate production rate and/or increased biomass yield, compared to a control E. coli (such as wild type E. coli) that lacks the one or more mutations in the one or more genes. Learn More

A U.S. Patent for "Filter Element and Filtration Assembly for Biopharmaceutical Applications" was awarded to 3M on
January 8th, 2019. The present invention are filter elements or cells and filtration assemblies or capsules for purifying and/or clarifying fluids used during various bioprocessing applications, for example, chromatography or depth filtration, and processes for making and using the same. A filter element comprises a media pack comprising two sides and a separator element located between the two sides, at least one side comprising a filtration media, wherein an inner edge periphery and/or an outer edge periphery of the filtration media comprise a bond directly to a portion of the separator element. Learn More

A U.S. Patent for "Reduced-Viscosity Concentrated Protein Formulations" was awarded to Genentech and Novartis on January 1st, 2019. The present invention concerns a method of lowering the viscosity of concentrated protein composition by: (1) increasing the total ionic strength of the formulation through the addition of salts or buffer components; or (2) altering the pH of the formulation to be lower (.apprxeq.4.0 to .apprxeq.5.3) or elevated (.apprxeq.6.5 to .apprxeq.12.0), without significantly compromising stability or biological activity. Accordingly, the invention concerns methods and means for reducing the viscosity of concentrated protein formulations, primarily to ensure easy manipulation before and during administration to a patient. Learn More

A U.S. Patent for a "Method for Connecting Modular Mobile Rooms" was awarded to G-CON Manufacturing on December 25th, 2018. The present invention is a method and a connector unit for connecting two or more structures wherein at least one of the two or more structures being connected is a mobile structure validatable for pharmaceutical manufacturing or patient care. The connector unit comprises of an alignment system, a sealing, and a fixation system. The method for using the connector unit comprises of aligning the two or more structures to dock the two or more structures together, sealing the two or more structures, or one or more structures and an environment air-tight with a seal or a sealing system... Learn More

A U.S. Patent for "Use of an Amino Acid Auxotrophy Cured Prokaryotic Strain for the Recombinant Production of a Polypeptide" was awarded to Roche on December 18th, 2018. The present invention is a method for producing a polypeptide in a prokaryotic cell, comprising the step of cultivating a prokaryotic cell comprising one or more nucleic acids encoding the polypeptide in a chemically defined minimal growth medium and recovering the polypeptide from the prokaryotic cell or the periplasm of the prokaryotic cell or from the medium, wherein the prokaryotic cell is an amino acid auxotrophy cured prokaryotic cell... Learn More

A U.S. Patent for a "Tri-Color Dual Glucose and Oxygen Sensors and Methods of Preparing and Using Them" was awarded to the Arizona Board of Regents on December 18th, 2018. The present invention relates to an optical fluorescence sensor comprising a probe for sensing glucose, an intra-reference probe, and a matrix. The present disclosure also relates to an optical fluorescence dual sensor comprising a probe for sensing glucose having two boronic acid moieties, a probe for sensing oxygen comprising modified porphyrin, an intra-reference probe that is rhodamine-based, and a matrix. The present disclosure additionally relates to methods of preparing these sensors and methods of using them. Learn More

A U.S. Patent Application for "Deep Eutectic Solvents and/or Ionic Liquids as Feed Media" was published by Merck KGaA on December 13th, 2018. The present invention is a liquid feed medium comprising a deep eutectic solvent and/or an ionic liquid. It has been found that deep eutectic solvents and/or ionic liquids can be used as highly concentrated liquid feeds for cell culture in bioreactors. Deep eutectic solvents and/or ionic liquids are per se liquid and do not need to be dissolved by the addition of a solvent which has no other effect on the cell culture than diluting it. At least one component of the deep eutectic solvent and/or ionic liquid is, favorably, a nutrient component... Learn More

A U.S. Patent Application for "Chromatography Columns, Systems and Methods" was published by GE on December 13th, 2018. The present invention is an axial flow chromatography column comprising: a housing comprising a side wall; axially spaced first and second end units positioned opposed to each other are separated by said side wall; a first filter which is adjacent to said first end unit and a second filter which is adjacent to said second end unit wherein said first and second filters together with the side wall define an enclosed bed space for containing a bed of particulate medium. Learn More

A U.S. Patent for "Bioreactor Systems with Tube Supports"
was awarded to Thermo Fisher on December 11th, 2018. The present invention is a bioreactor system that includes a support housing having an interior surface bounding a compartment, the support housing having a sidewall with an opening extending therethrough and communicating with the compartment. A rack is secured to the support housing in alignment with the opening. An elongated first shaft has a first end and an opposing second end, the first end of the first shaft being secured to the rack so that the second end of the first shaft projects outwardly away from the sidewall of the support housing. Learn More

A U.S. Patent for "Control of pH and Dissolved Gas in Medium" was awarded to Kuros Biosciences on December 11th, 2018. The present invention is a method and device to adjust the concentration of at least two gasses in a fluid, wherein the method involves adjusting the concentration of a first gas in the fluid by adjusting the concentration of a second gas in the fluid wherein the first and second gases are kept separate until dissolved in the fluid. Learn More

A U.S. Patent Appliction for a "Cell Culture Medium" was
published by Boehringer Ingelheim on December 6th, 2018. The present invention provides a basal cell culture medium and a feed medium with novel amino acid ratios and/or iron choline citrate as iron carrier that result in improved performance of mammalian cell culture processes, such as CHO cultivation and protein production processes, in particular in increased product titer (e.g. of monoclonal antibodies). Also provided are methods for culturing mammalian cells and producing a protein of interest using said basal cell culture medium and optionally feed medium. Learn More

A U.S. Patent for "Methods of Cell Culture" was awarded to Momenta on December 4th, 2018. The present invention is a method of producing an adalimumab preparation having a target value of one or more of fucosylated glycans, galactosylated glycans, high mannose glycans, and sialylated glycans, the method comprising: (a) providing a cell genetically engineered to express adalimumab; (b) culturing the cell in a culture medium under conditions in which the cell expresses the adalimumab; and (c) harvesting a preparation of the adalimumab produced by the cell that meets the target value of the one or more of fucosylated glycans, galactosylated glycans, high mannose glycans, and sialylated glycans... Learn More

A U.S. Patent Application for a "Method for Controlling Continuous Chromatography and Multi-Column Chromatography Arrangement" was published by the Karlsruher Institute on November 29th, 2018. The present invention is a method of controlling at least one multi-column chromatography arrangement for the continuous process of purifying biopharmaceuticals are provided. The methods include introducing a first multi-component mixture into a column of the multi-column chromatography arrangement; detecting at least one multivariate signal by at least one detector; calculating at least one process parameter on the basis of the multivariate signal... Learn More

A U.S. Patent Application for "Production of Viruses in Cell Culture" was published by the University of Georgia on November 29th, 2018. The present invention relates to methods of replicating viruses in vitro. In particular, the invention relates to a genetically modified population of cells, and/or a population of cells treated with an exogenous compound, wherein the cells are capable of producing more virus than cells lacking the genetic modification and/or lacking treatment with the exogenous compound. The invention also relates to methods of producing populations of such cells, as well as the use of the viruses obtained to prepare vaccine compositions. Learn More

A U.S. Patent for "Temperature Shift for High Yield Expression of Polypeptides in Yeast and Other Transformed Cells" was awarded to Alder Bio on November 27th, 2018. The present invention is a method of producing a recombinant multi-subunit complex in yeast cells, comprising: (a) culturing at a first temperature yeast cells comprising one or more genes that encode said multi-subunit complex; and (b) culturing said yeast cells at a second temperature and allowing said yeast cells to produce said multi-subunit complex; wherein said first temperature is between 27.5.degrees C and 28.5.degrees C and said second temperature is between 30 degrees C and 31 degrees C. Learn More

A U.S. Patent for a "Process for Protein Production" was awarded to Novo Nordisk on November 27th, 2018. The present invention relates to a process for the production of a haemostasis protein by continuous perfusion culturing of a cell culture in suspension, said cell culture expressing said haemostasis protein into said culture suspension, wherein the cell culture flows across a filter module, which filter module leads to a harvest port, the filter module having a mesh size of from 0.1 to 2.9 .mu.m allowing passage across of the haemostasis protein and wherein the flow across the filter module is an alternating tangential flow. The invention also relates to a protein produced by the process of the invention. Learn More

A U.S. Patent for "Devices, Systems and Methods for Automated Cell Culturing" was awarded to GE on November 20th, 2018. The present invention is a multi-scalable bioreactor, which comprises a culture vessel for seeding and culturing cells by adding a cell-culture media, wherein the culture vessel comprises at least a side wall and a bottom surface, a specific heat transfer area and a specific gas transfer area; wherein the culture vessel is configured to accommodate the cell-culture media volume up to 10 liters, and wherein the specific heat transfer area and the specific gas transfer area are independent of cell-culture media volume... Learn More

was awarded to Sanofi on November 20th, 2018. The present invention is a method for purifying a monoclonal antibody or antigen-binding fragment thereof from solution comprising: (a) a first chromatography step comprising: passing said solution over a first Protein A affinity chromatography column; and eluting a crude protein eluent from the first chromatography column using a first elution buffer; and (b) a second chromatography step comprising: passing the crude protein eluent obtained at the end of step (a) over a second chromatography column; and recovering purified monoclonal antibody... Learn More

A U.S. Patent Application for a "Mixing Device for Mixing the Content of a Bioreactor, Comprising a Synchronization Mechanism" was published by Sartorius Stedim on November 15th, 2018. The present invention relates to a system comprising a mixing device for mixing the content of a bioreactor. The system comprises at least one movement device for initiating a mixing movement in the bioreactor or in a holder for receiving a bioreactor and at least one sensor that can be arranged in or on the bioreactor for receiving at least one physiological or physical measurement variable. The mixing device or the bioreactor further comprises a sensor or transmitter for generating a synchronization measurement variable. Learn More

A U.S. Patent Application for "Methods for the Harvesting of Cell Cultures" was published by Millenium Pharma, Now Takeda, on November 15th, 2018. The present invention provides methods for optimization of the harvest process by clarification of cell samples using centrifugation and depth filtration. The present invention provides methods for the determination of the optimal ratio of Q/.SIGMA. for the centrifugation step of a harvest process of a cell culture. The present invention provides methods for the determination of the number of particles and the size of the particles in the centrate of a centrifugation step of a harvest process of a cell culture by the use of imaging technology. Learn More

was awarded to Carbios on November 13th, 2018. The present invention is a method for recycling at least one plastic product comprising at least partially semi-crystalline polyethylene terephthalate (PET) and at least one other additive, the method comprising: providing, without sorting, a mixture of plastic products coming from plastic waste collection and comprising said at least one plastic product; selecting a degrading enzyme suitable for degrading the at least partially semi-crystalline PET of the at least one plastic product; depolymerizing the at least partially semi-crystalline PET of the plastic product to monomers... Learn More

A U.S. Patent for a "Method for Recycling Plastic Products"
was awarded to Carbios on November 13th, 2018. The present invention is a method for recycling at least one plastic product comprising at least partially semi-crystalline polyethylene terephthalate (PET) and at least one other additive, the method comprising: providing, without sorting, a mixture of plastic products coming from plastic waste collection and comprising said at least one plastic product; selecting a degrading enzyme suitable for degrading the at least partially semi-crystalline PET of the at least one plastic product; depolymerizing the at least partially semi-crystalline PET of the plastic product to monomers... Learn More

A U.S. Patent Application for a "Probe Port and Related Container System" was published by Life Technologies on November 8th, 2018. The present invention is a container system that includes a flexible bag, a probe port, and a probe. The probe port includes an elongated tubular member having an interior surface bounding a first passageway extending between a first end and an opposing second end, the tubular member being made of a polymeric or elastomeric material and being flexible. The probe port also includes a flange coupled to the tubular member and radially outwardly projecting therefrom, the flange being secured to the flexible bag... Learn More

A U.S. Patent for a "Fluid Mixing System with Steady Support" was awarded to Life Technologies on November 6th, 2018. The present invention is a fluid mixing system that includes a flexible bag having a first end, an opposing second end, and an interior surface bounding a compartment. A first rotational assembly includes a first casing mounted to the first end of the flexible bag and a first hub rotatably mounted to the first casing. A second rotational assembly includes a second casing mounted to the second end of the flexible bag and a second hub rotatably mounted to the second casing. A connector has a first end coupled with the first hub and an opposing second end. Learn More

A U.S. Patent for a "Modified Serum-Free Cell Culture Medium" was awarded to AbbVie on November 6th, 2018. The present invention provides improved salt-free basal growth media for use in mammalian cell culture. The invention includes a serum-free cell culture medium comprising Part A, Part B, and Part C, wherein Part A consists essentially of a modified basal medium which excludes the following components: sodium bicarbonate, a buffer, mono-basic sodium phosphate, di-basic sodium phosphate, an osmolarity regulator, a surfactant, and monosaccharide glucose; Part B consists essentially of an inorganic iron source. Learn More

A U.S. Patent for a "Coriolis Flow Meter for Measuring Properties of a Fluid and Method Therefor" was awarded to GE on November 6th, 2018. The present invention is a coriolis flow meter for measuring one or more properties of a fluid is described herein which involves a modular configuration, and includes a fluid flow sub-system and a mechanical oscillator sub-system, both functionally separate, and are coupled in a closed loop arrangement, such that the flow conduit is not directly vibrated, and instead receives induced oscillations from the mechanical oscillator sub-system. The Coriolis flow meter is useful for high purity applications, as well as for the bioprocessing applications. Learn More

A U.S. Patent Application for a "Novel Cell Line Screening Method" was published by Larix Biosciences on November 1st, 2018. The present invention invention provides a novel cell line development method useful to screen for recombinant protein production. The method utilizes a membrane-anchored reporter or an intracellular reporter residing in the expression vector for a gene of interest to facilitate initial cell selection by FACS or MACS. A switching mechanism can be used to delete the reporter from the chromosome by providing an appropriate DNA recombinase, which turns the selected cells into production cells that secrete the protein of interest without co-expression of the reporter. Learn More

A U.S. Patent Application for a "Method and Chromatography Medium" was published by GE on October 25th, 2018. The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media. Learn More

A U.S. Patent for a "Method and Apparatus for Antibody Production and Purification" was published by Biovest on October 9th, 2018. The present invention pertains to methods and apparatus for the production and purification of cell products, such as immunoglobulins. One aspect of the invention is an integrated cell culture and purification apparatus for the growth and maintenance of cells and the harvest and purification of cell products, such as immunoglobulins. Thus, the apparatus integrates a cell culture function with a purification function. Other aspects of the invention pertain to an automated method for producing immunogenic compositions such as vaccines. Learn More

A U.S. Patent for a "Cell Culturing System for Cultivating Adherent Cells and Liquid Supply Interface Comprising a Cell Culture Container" was published by Hamilton on October 9th, 2018. The present invention relates to improvements in cell culturing systems comprising at least one cell culture container for collection and supply, i.e. particularly for cultivating with the goal of propagation, of adherent cells therein, comprising a nutrient medium reservoir for supplying the adherent cells in the cell culture container with nutrients, having a cleaning fluid reservoir for cleaning fluid flow paths or/and flow areas with the cleaning fluid, and comprising a liquid supply interface for coupling to the cell culture container. Learn More

A U.S. Patent for a "Device for Delivery of Sample Fluid" was published by GE on October 9th, 2018. The present invention relates to a device for delivery of fluid, said device comprising at least two fluid ducts of flexible material, each of which in one end can be connected to a fluid source and in the opposite end is connected to a manifold having an inlet end and an outlet end, each of said fluid ducts comprising a pinch valve for closing and opening the duct. According to the invention a check valve is disposed in at least one of said fluid ducts in the end thereof connected to the manifold. Learn More

A U.S. Patent for a "Contaminant Removal Method" was awarded to CSL on October 2nd, 2018. The present invention is a method for purifying Apo A-I is provided including the steps of providing a solution comprising Apo A-I and guanidine hydrochloride and filtering the solution through a filter having a pore size in a range from 15 nrn to 35 nm to thereby reduce viral contamination of the Apo A-I. An Apo A-f preparation is provided having at least a 12 log LRV (log reduction value) for a parvovirus; and/or at least 9 log LRV for a non-enveloped virus; and/or at least 8.5 log LRV for a lipid enveloped virus. Learn More

A U.S. Patent for a "Device, System and Process for Modification or Concentration of Cell-Depleted Fluid"
was awarded to Repligen on September 25th, 2018. The present invention is a product concentration device that utilizes a reservoir connected to a hollow-fiber filter element where the reservoir can serve as a container for filtrate emanating from another filtering device, such that product in the reservoir can be stored, concentrated and/or further processed as desired. Enclosed reactor systems, each of at least three chambers, fluid flow between the chambers controlled by selectively permeable barriers, flow controlled by an alternating flow diaphragm pump. Learn More

A U.S. Patent for "Vertical Wheel Bioreactors" was awarded to PBS on September 25th, 2018. The present invention is a pneumatic bioreactor includes a vessel containing a fluid to be mixed and at least one mixing device driven by gas pressure. A first embodiment includes a floating impeller that rises and falls in the fluid as gas bubbles carry it upward to the surface where the gas is then vented, permitting the impeller to sink in the fluid. The floating impeller may be tethered to a second impeller with a flexible member and pulley. The mixing speed is controlled with electromagnets in the vessel acting upon magnetic material in the impeller or its guides... Learn More

A U.S. Patent for a "Fuser and Sealer Integrated System"
was awarded to GE on September 25th, 2018. The present invention is an integrated system for fusing and sealing a plurality of tubes, the integrated system comprises: at least one fusing unit, each fusing unit comprising at least two holders, wherein each holder is capable of receiving and holding a portion of a tube of the plurality of tubes, a cutting blade for cutting a portion of the tube received in each holder of the at least two holders simultaneously, and a driving assembly comprises a first cam member operatively connected to the cutting blade and a second cam member operatively connected... Learn More

A U.S. Patent Application for "Automated Batch Data Analysis" was published by Lonza on September 20th, 2018. The present invention is a system for automated batch data analysis. The system for automated batch data analysis includes, for example, at least one system component and a database. The system also includes at least one computing device for executing stored programmable instructions to: acquire data associated with the system component, determine which data from the received data are identified for extraction based at least in part on a tag, and extract the tagged data based on the determination. Learn More

A U.S. Patent Application for "Cells and Method of Cell Culture" was published by Pfizer on September 20th, 2018. The present invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods. Learn More

A U.S. Patent for a "Combination Stirrer" was awarded to Roche on September 18th, 2018. The present invention is a device comprising an agitator and a dialysis module within a cultivation vessel, wherein the agitator comprises: a shaft comprising a shaft axis; one anchor impeller comprising at least two opposing stirrer blades, wherein the stirrer blades of the anchor impeller have no pitch with respect to the shaft axis; and one or more inclined-blade stirrers each comprising at least two stirrer blades, wherein the stirrer blades of each inclined-blade stirrer have a pitch with respect to the shaft axis; wherein the stirrer blades of the anchor impeller are parallel to each other... Learn More

A U.S. Patent for an "Apparatus for Automated Determining of at Least Two Different Process Parameters" was awarded to Endress+Hauser on September 18th, 2018. The present invention is an apparatus for automated determining of at least two different process parameters of a process liquid of a process, especially a bioprocess, comprising: a first measuring cell, which is embodied to provide a first measurement signal dependent on a first process parameter of a first sample of the process liquid; a second measuring cell, which is embodied to provide a second measurement signal dependent on a second process parameter of a second sample... Learn More

A U.S. Patent Application for "Wireless Sensor Information Monitoring" was published by Lonza on September 13th, 2018. The present invention is a system, apparatus, and method for continuously monitoring data output from a wireless sensor on a mesh network at a frequency higher than mesh formation/propagation. For example, the system may include a wireless gateway, an amplifier coupled to the wireless gateway, a first antenna coupled to the amplifier, a second antenna coupled to the amplifier, and a wireless sensor. The wireless sensor may be a wireless temperature sensor configured to monitor the temperature of a cryopreserved material product. Learn More

A U.S. Patent for a "Liquid Cation Exchanger" was awarded to Evonik on September 11th, 2018. The present invention relates to a process for removing an organic compound having one or more positive charges from an aqueous solution, comprising the steps a) provision of the aqueous solution comprising the organic compound and of a hydrophobic organic solution which comprises a liquid cation exchanger, where the liquid cation exchanger is hydrophobic, and where the liquid cation exchanger has one or more negative charges and an overall negative charge, b) contacting the aqueous solution and the organic solution... Learn More

A U.S. Patent for "Sterile Affinity Chromatography Resin"
was awarded to Genzyme on September 11th, 2018. The present invention provides methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chrom resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation... Learn More

A U.S. Patent Application for a "Method for Cell Line Development" was published by GE on September 6th, 2018. The present invention relates to an improved method for cell line development which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI), novel design of the GOI genome target location and the introduction... Learn More

A U.S. Patent for "Methods, Surface Modified Plates and Compositions for Cell Attachment, Cultivation and Detachment" was awarded to Janssen Biotech on September 4th, 2018. The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells...
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A U.S. Patent for "Cell Culture Media and Methods"
was awarded to Life Technologies on September 4th, 2018. The present invention describes compositions and methods for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release... Learn More

A U.S. Patent for "Methods of Producing Two Chain Proteins in Bacteria" was awarded to Genentech on September 4th, 2018. The present invention provides for methods of producing a recombinant polypeptide containing two chains, such as an immune mobilizing monoclonal T-cell receptor against cancer (ImmTAC) protein including an alpha chain and a beta chain. In particular, methods are provided for producing heterologous secretory proteins in bacteria through utilization of optimized expression vectors and culture processes. Learn More

A U.S. Patent for "High pH Protein Refolding Methods"
was awarded to Bristol-Myers Squibb on September 4th, 2018. The present invention is a method for refolding a denatured protein, comprising suspending a denatured protein in a suspension solution to obtain a composition comprising suspended denatured proteins; combining the composition comprising suspended denatured proteins with a solubilization buffer having a pH in the range of 11.5 to 12.8 to thereby obtain a composition comprising solubilized denatured proteins; and combining the composition comprising solubilized denatured proteins with a refold buffer having a pH in the range of 10 to 10.9. Learn More

A U.S. Patent for a "Regulated Vacuum Off-Gassing of Gas Filter for Fluid Processing System and Related Methods"
was awarded to Life Technologies on August 28th, 2018.
The present invention is a method for filtering a gas, the method comprising: delivering a gas into a compartment of a gas filter assembly, the compartment being at least partially bounded by a casing comprised of a flexible sheet of polymeric film; and applying a partial vacuum to the gas filter assembly so that the partial vacuum assists in drawing the gas through a porous filter body of the gas filter assembly that is at least partially disposed within the compartment of the gas filter assembly. Learn More

A U.S. Patent for a "Cell Culture Method in a Bioreactor
System Using Micro-Carriers" was awarded to PBS Biotech on August 28th, 2018. The present invention is a
system and method for compartmentalizing micro-carriers in a bioreactor includes a container configured to store micro-carriers and a vessel configured to contain a fluid so as to prevent the micro-carriers from entering the vessel during sterilization, shipping, storage, or other pre-use handling of the system. One or more addition lines connect the container and the vessel such that the vessel is in fluid communication with the vessel. Learn More

A U.S. Patent for "Disposable Bioreactors and Methods for Construction and Use Thereof" was awarded to Distek on August 28th, 2018. The present invention is a disposable bioreactor including a headplate and a stirrer. The headplate has at least one inlet port aperture and one outlet port aperture formed therein and is adapted to couple sealingly with a bag capable of receiving a culture medium. The stirrer is coupled to and extends from the headplate and is adapted to stir the culture medium when the headplate is coupled to the bag. The bioreactor is adapted to fit within the upper opening of the stand of an existing conventional glass bioreactor, so that the bag is suspended within the vessel.
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A U.S. Patent for "Mammalian Cell Culture Processes for Protein Production" was awarded to Bristol-Myers Squibb on August 28th, 2018. The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise the addition of glucocorticoid compound during the culturing period. The addition of glucocorticoid compound sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein. Learn More

A U.S. Patent Application for a "Disposable Bioprocess System Supporting Biological Activity" was awarded to Stobbe Pharma Tech on August 23rd, 2018. The present invention is a Disposable Bioprocess System including a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. Learn More

A U.S. Patent for an "Audio Identification Device, Audio Identification Method and Audio Identification System"
was awarded to Sartorius Stedim on August 21st, 2018. The present invention is an audio identification device. The audio identification device comprises: at least one sensing device configured to capture an audio signal present in a working space of a processing setup including at least one processing device; and a processor configured to: receive information related to the at least one processing device; retrieve audio data stored in a database using the received information related to the at least one processing device; identify a state of the at least one processing device.
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A U.S. Patent for a "Particle Sensor with Interferent Discrimination" was awarded to BugLab on August 21st, 2018. The present invention provides methods and devices to measure particle suspension concentrations in the presence of potential interferents. Particle back-scatter readings are taken at light wavelengths that are absorbed by the medium before interacting with surrounding objects. Source-detector spacings are minimized compared to the mean absorbance path length of light, thereby maximizing the range of sensitivity to particle concentration. Discrimination against potentially interfering particles, such as bubbles, is provided... Learn More

A U.S. Patent Application for a "Virtual Transmitter for Bioreactor Automation System" was awarded to Finesse Solutions on August 16th, 2018. The present invention is a bioreactor monitoring and/or control system utilizing only non-dedicated user input and information display devices, a digital controller and software, said system comprising: i) at least one diagnostic probe for measuring operating conditions in a bioreactor; ii) signal conditioning and communication electronics which supply operating current and/or voltage to said probe and which convert the diagnostic signal into a format accessible. Learn More

A U.S. Patent Application for a "Cell Culture System and Method" was awarded to Lonza on August 16th, 2018.
The present invention is a bioprocess system and a method for incubating, growing and harvesting cell cultures is described. Also disclosed is a bioprocess container that can be used with the system. In one aspect of the present disclosure, the bioprocess system includes bioprocess tubes and cell culture tubes having particular dimensions and being made from specific materials that allow the tubes to be welded together while preventing open connections and/or ruptures. Learn More

A U.S. Patent for a "Flexible Bag" was awarded to GE
on August 14th, 2018. The present invention is a flexible bag and a method for filling a flexible bag which is collapsed when empty, wherein at least one fastening means is provided on a surface of the flexible bag such that at least two upper parts of the flexible bag can be held together by this at least one fastening means during a first part of filling of the flexible bag, whereby the fastening means is designed to release when the bag is filled to a certain amount such that the two upper parts being held together by the fastening means will lose contact. Learn More

A U.S. Patent for a "Process for expression of recombinant proteins in Pichia pastoris using a fed batch model" was awarded to Biocon on August 14th, 2018. The present invention is a comprehensive model for expression of recombinant peptides by Pichia pastoris. The model uses an easily controllable variable called `critical nutrient ratio` for obtaining a right balance between product synthesis and it's degradation during the fermentation process. The extra cellular concentration of precursor could be increased by about 10 folds and the degradation constants could be reduced by about 10-20 folds for intracellular and extracellular cases respectively by controlling critical nutrient ratio and addition of soya flour hydrolysate and EDTA. Learn More

A U.S. Patent for "Chromatography columns, systems and methods" was awarded to GE on August 14th, 2018. The present invention is an axial flow chromatography column comprising: a housing comprising a side wall; axially spaced first and second end units positioned opposed to each other are separated by said side wall; a first filter which is adjacent to said first end unit and a second filter which is adjacent to said second end unit wherein said first and second filters together with the side wall define an enclosed bed space for containing a bed of particulate medium therein; the first end unit including a first port which is in fluid communication with said enclosed bed space for adding a liquid. Learn More

A U.S. Patent Application for a "Method and System for Interaction Analysis" was published by GE on August 9th, 2018. The present invention relates to a method of evaluation of molecular binding interactions at a sensing surface, and more particularly to a method for evaluation of screening data obtained from an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor that is independent of interaction models. Preferably the biosensor is a SPR biosensor. The invention also relates to a biosensor system to perform the method and a computer program to control the operation of the biosensor system. Learn More

A U.S. Patent Application for a "Therapeutic Cell Washing, Concentration, and Separation Utilizing Acoustophoresis"
was published by FloDesign Sonics on August 9th, 2018. The present invention is a method of washing particles, the method comprising: providing an initial mixture of a first media and particles to a chamber of an acoustophoretic device, the acoustophoretic device including at least one ultrasonic transducer that includes a piezoelectric material; driving the at least one ultrasonic transducer to create an acoustic standing wave in the chamber, such that at least a portion of the particles are retained in the acoustic standing wave... Learn More

A U.S. Patent Application for a "Process for Producing Cell Culture Media" was published by Merck Germany on August 9th, 2018. The present invention is a method for improving the solubility of a dry powder cell culture medium by a) identifying the one or more poorly soluble components in the said cell culture medium b) co-lyophilizing the components identified in step a) with at least one other component of said cell culture medium as a carrier c) preparing the said dry powder cell culture medium by mixing the one or more co-lyophilisates generated in step b) with the other components of the cell culture medium and optionally milling the resulting mixture. Learn More

A U.S. Patent for a "Method of Producing Erythrocytes Without Feeder Cells" was awarded to Celularity on August 7th, 2018. The present invention is a method of producing erythrocytes, comprising: a) expanding a first population of hematopoietic cells in a first medium in the absence of feeder cells to obtain an expanded population of hematopoietic cells, wherein the first population of hematopoietic cells has an initial cell density of 5 times 10 sup 4 cells/mL or less and the first medium comprises Epo at a concentration of 1 IU/mL or less and SCF at a concentration of 100 ng/mL or more... Learn More

A U.S. Patent for "Expression of Mammalian Proteins in Pseudomonas Fluorescens" was awarded to Pfenex on August 7th, 2018. The present invention is a process for increasing expression of a recombinant cytokine or chemokine comprising: (a) growing a Pseudomonas fluorescens (P. fluorescens) host cell transformed with a nucleic acid comprising a sequence encoding the recombinant cytokine or chemokine under conditions that allow expression of the recombinant cytokine or chemokine, wherein the nucleic acid does not comprise a secretion signal coding sequence; and (b) isolating the recombinant cytokine or chemokine expressed... Learn More

A U.S. Patent Application for an "Inducible Eukaryotic Expression System" was published by Regeneron on August 2nd, 2018. The present invention is a method for producing a protein of interest o a gene of interest in eukaryotic cells in a bioreactor, comprising: (a) seeding a starter culture for a bioreactor with eukaryotic cells in the presence of a concentration of a ligand that stabilizes a regulatory fusion protein (RFP) and inhibits expression of a protein of interest, wherein the eukaryotic cells comprise i. a promoter operably linked to the gene of interest encoding the protein of interest and controlled by a Tet or Arc operator operably linked and positioned... Learn More

A U.S. Patent Application for the "Identification of Calibration Deviations of pH Measuring Devices" was published by Roche on August 2nd, 2018. The present invention is a method for determining if a first pH measuring device operatively coupled to a first tank is affected by a pH-measuring problem, the problem being that the first pH measuring device is calibrated differently than a second pH measuring device operatively coupled to a second tank, the method comprising: receiving, by a comparison unit, a first CO2 concentration and a first pH value, the first CO2 concentration being a CO2 concentration of a first gas volume above a medium in the first tank... Learn More

A U.S. Patent Application for the "Removal of Protein Aggregates from Biopharmaceutical Preparations in a Flow-Through Mode" was published by Merck Germany on August 2nd, 2018. The present invention is a flow-through process for purifying a target molecule from a Protein A eluate comprising the steps of: (a) contacting the eluate recovered from a Protein A chromatography column with activated carbon; (b) contacting a flow-through sample from step (a) with an anion exchange chromatography media; and (c) contacting a flow-through sample from step (b) with a solid support comprising one or more cation exchange binding groups attached thereto... Learn More

A U.S. Patent for "Methods of Using Polypeptides Having Cellulolytic Enhancing Activity" was awarded to Novozymes on July 31st, 2018. The present invention is a method for degrading a cellulosic material, comprising: treating the cellulosic material with an enzyme composition comprising a GH61 polypeptide having cellulolytic enhancing activity, wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of: (a) a GH61 polypeptide having at least 90% sequence identity to amino acids 21 to 322 of the polypeptide... Learn More

A U.S. Patent Application for "Uses of Monoclonal Antibody 8H9" was published by the Sloan-Kettering Institute on July 26th, 2018. The present invention provides a composition comprising an effective amount of monoclonal antibody 8H9 or a derivative thereof and a suitable carrier. This invention provides a pharmaceutical composition comprising an effective amount of monoclonal antibody 8H9 or a derivative thereof and a pharmaceutically acceptable carrier. This invention also provides an antibody other than the monoclonal antibody 8H9 comprising the complementary determining regions of monoclonal antibody 8H9 or a derivative thereof... Learn More

A U.S. Patent Application for "Methods and Systems for Using a Virtual or Augmented Reality Display to Perform Industrial Maintenance" was published by Lonza on July 26th, 2018. The present invention is a method of providing a virtual reality or augmented reality display is provided. The method includes generating, with a camera of a device, first video content (e.g., a first video stream) that may include a depiction of a component of a facility for the processing of a pharmaceutical product, e.g., a biological product and detecting or selecting the component (e.g., a vessel, a pipe between a holding tank and a filter). Learn More

A U.S. Patent for "Cell Culture Media" was awarded to Merck KGaA on July 24th, 2018. The present invention relates to cell culture media comprising inorganic ester derivatives of tyrosine and/or cysteine. The poor solubility of tyrosine and the often non-sufficient stability of cysteine in cell culture media is overcome by substituting them with an inorganic ester derivative, e.g. with a phosphorylated derivative. Learn More

A U.S. Patent for "Monitoring of the Freezing State of a Biopharmaceutical Fluid Found in a Container" was awarded to Sartorius Stedim on July 24th, 2018. The present invention is a monitoring device for monitoring the freezing state of a biopharmaceutical fluid in a container intended to receive a biopharmaceutical fluid that is to transition between the liquid state and the frozen state, wherein the device comprises a peripheral envelope made of plastic, intended and suitable for association with a thermal treatment receptacle, wherein the device further comprises a sensor suitable for non-intrusively detecting a control parameter which is a macroscopic parameter... Learn More

A U.S. Patent for a "Mammalian Cell Culture Processes for Protein Production" was awarded to BMS on July 24th, 2018. The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise the addition of glucocorticoid compound during the culturing period. The addition of glucocorticoid compound sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein. Learn More

A U.S. Patent Application for "Cell Line 3M" was published by Merck on July 19th, 2018. The present invention provides an isolated Chinese hamster ovary (CHO) cell produced by a method comprising: adapting CHO-DXB11 cells into animal-component free medium in suspension, e.g., for about 83 days; then subcloning the cells into said medium, e.g., twice. In an embodiment of the invention, the subcloning steps comprise (a) serially diluting the adapted cells (e.g., by 1/2 each dilution) in growth medium, for example, in about 100 microliters, e.g., in a microtiter dish comprising, for example, 96 wells; (b) allowing the cells of each dilution to grow... Learn More

A U.S. Patent Application for "Heatseal Sampling"
was published by GE on July 19th, 2018. The present invention is a device for taking a sample from a liquid cell culture in a bioreactor wherein said device comprises: (i) a hollow body comprising outer walls that define a fluid path therethrough wherein said outer walls are at least partly comprised of a sealable material, wherein said fluid path begins at an upstream end and ends at a downstream end of said hollow body, and wherein said upstream and downstream ends are adapted to fluidly connect said fluid path with the interior of said bioreactor; and, (ii) a sealing means which is adapted to seal the perimeter... Learn More

A U.S. Patent for "Gas Delivery Devices and Associated Systems and Methods" was awarded to Biogen on July 17th, 2018. The present invention is a device for producing bubbles of a gas within a liquid, comprising: a primary conduit comprising a plurality of openings fluidically connecting an internal flow pathway of the primary conduit to an environment outside the primary conduit; and a secondary conduit located at least partially within the primary conduit, the secondary conduit configured to redistribute the pressure of the gas delivered to the openings of the primary conduit, and comprising a plurality of openings fluidically connecting an internal flow pathway... Learn More

A U.S. Patent for "Single Use Aseptic Fluid Couplings" was awarded to Colder on July 17th, 2018. The present invention is a fluid coupling device, comprising: a male connector component defining a first male connector internal space and defining a second male connector internal space, the male connector component comprising: a male coupling shuttle valve member partially disposed within the first male connector internal space and partially disposed within the second male connector internal space; and a locking sleeve movably coupled to other portions of the male connector component; and a female connector component releasably matable with the male connector... Learn More

A U.S. Patent for "Protein Purification Methods to Remove Impurities" was awarded to Amgen on July 17th, 2018.
The present invention is a method of reducing high molecular weight species in a protein sample comprising adalimumab or a biosimilar thereof comprising: (a) subjecting the protein sample to mixed mode chromatography (MMC) to form a first eluate using a washing buffer comprising Tris-HCl and NaCl, wherein the protein sample subjected to MMC has a pH of about 6.9 to about 7.3 and a conductivity at a temperature between about 18.degree. C. and about 22.degree. C. of about 15 to about 30... Learn More

A U.S. Patent Application for a "Bioprocess Method and System" was published by Synthace on July 12th,
2018. The present invention is a system for performing a biological process that includes a step involving one or more of a reproducible and scalable transformation of a material into a physical product or a quantification of an amount of a molecule in the material, the system comprising: (i) a server with at least one processing module adapted to implement a method for performing the biological process wherein the method comprises implementation of at least one unit operation, and wherein the at least one unit operation is defined according to a standardized element... Learn More

A U.S. Patent Application for "Methods for Producing Virus for Vaccine Production" was published by Takeda on July 12th, 2018. The present invention is a method for producing an Enterovirus A virus, comprising: (a) culturing an adherent cell in a fixed bed comprising a macrocarrier, wherein the cell is cultured in a first cell culture medium; (b) inoculating the cell with the Enterovirus A virus under conditions in which the Enterovirus A virus infects the cell; (c) culturing the infected cell in the fixed bed in a second cell culture medium under conditions in which the infected cell produces the Enterovirus A virus; and (d) harvesting the Enterovirus A virus produced by the cell. Learn More

A U.S. Patent Application for "Bispecific Binding Proteins"
was published by Abbvie on July 12th, 2018. The present invention provides bispecific binding proteins capable of specifically binding two antigens. The binding proteins generally comprise variable light and variable heavy chain regions or domains that correspond to variable light and variable heavy chain regions of immunoglobulins. At least one antigen binding moiety of the binding proteins is in a single chain format known in the art as an scFv. In some embodiments, the other antigen binding moiety comprises an IgG. In other embodiments, the other antigen binding moiety comprises an scFv. Learn More

A U.S. Patent for " Enhancing Ingredients for Protein Production from Various Cells" was awarded to Nissan Chemical Industries on July 10th, 2018. The present invention is a method of producing a protein by using an animal cell culture, comprising cultivating an animal cell in a medium comprising 0.01% to 0.05% deacylated gellan gum in a culture tool, wherein the animal cell is a Chinese Hamster Ovary (CHO) cell, a human cancer cell, or a hybridoma, into which a gene encoding the protein has been introduced, wherein the protein is an immunity related protein, a protein relating to cell proliferation, differentiation, or a membrane protein, wherein the culture tool is composed of polystyrene, thereby producing the protein. Learn More

A U.S. Patent Application for a "Cost-Effective Raman Probe Assembly for Single-Use Bioreactor Vessels" was published by Kaiser Optical Systems on July 5th, 2018. The present invention addresses systems and methods used to couple an optical sampling probe to a port in a single use bioreactor bag for in-process monitoring. A combination of re-useable and disposable components maintain precision while reducing costs. A disposable barb with an integral window, received by the port of the reaction vessel, is coupled to a re-useable optic component with a focusing lens. A separate focus alignment tool is used to set the lens position to a precise focal point before placement... Learn More

A U.S. Patent Application for an "Optical Device for In-Line and Real-Time Monitoring of Microorganisms" was published by the University of Arizona on July 5th, 2018. The present invention is a multi-wavelength laser diode based optical sensor system capable of monitoring the dynamics and physiological changes of a microorganism culture in real-time. The microorganism culture from a microorganism production chamber is pumped to a flow chamber. Laser diodes emit light at certain wavelengths through the flow chamber, which is sensed by photodiodes. A laser control circuitry is operatively connected to the laser diodes and a signal conditioning circuitry... Learn More

A U.S. Patent Application for a "Cell Culture Process"
was published by Momenta Pharma on July 5th, 2018. The present invention is a method of manufacturing a preparation of adalimumab, comprising: culturing a cell in a medium comprising 1.5 g/L arginine to 20 g/L arginine under conditions in which the cell expresses adalimumab; isolating the adalimumab, thereby producing a preparation of adalimumab; and formulating the preparation into a drug product if the preparation meets a target value of one or more C-terminal variants of adalimumab, wherein the C-terminal variants differ in amino acid sequence only by the presence or absence of a lysine... Learn More

A U.S. Patent Application for "Enhanced Purification of Antibodies and Antibody Fragments by Apatite Chromatography" was published by Bio-Rad on July 5th, 2018. The present invention relates in certain embodiments to methods for enhancing purification of antibodies and immunoreactive antibody fragments by apatite chromatography in the presence of one or more of borate compounds, sulfate compounds, monocarboxylate compounds, and/or in the presence of calcium compounds. In certain embodiments, the invention may permit more effective separation of intact non-aggregated antibody from unwanted fragments, aggregated antibody, and other contaminants. Learn More

A U.S. Patent for "Large Volume Disposable Ultrafiltration Systems and Methods" was awarded to GE on July 3rd, 2018. The present invention is a disposable filtration system that can include a disposable filter housing having an ultrafiltration filter contained therein. The disposable filtration system can further include a feed inlet and retentate outlet in fluid communication on one side of the filter and a permeate conduit on the other side of the filter for withdrawing permeate that passes through the filter. The system can include at least one disposable pressure control valve in fluid communication with either the retentate outlet or the permeate conduit and configured to regulate the pressure within the housing. Learn More

A U.S. Patent Application for a "Method for Development of Recombinant Proteins with Fingerprint Like Similarity to the Reference Product" was published by STC Biologics on June 28th, 2018. The present invention relates to the methods of developing recombinant proteins with a fingerprint like similarity to the reference product or the originator. The method is particularly useful in the development of biosimilar products. This method can also be used to establish comparability during the manufacturing process change for the originator products. Hie methods described herein are used to obtain a recipe for the production of a biosimilar product... Learn More

A U.S. Patent Application for "Digestible Substrates for Cell Culture" was published by Corning on June 28th, 2018. The present invention is a cell culture article, comprising: a substrate comprising a polygalacturonic acid compound selected from at least one of: pectic acid or salts thereof, and partially esterified pectic acid having a degree of esterification from 1 to 40 mol % or salts thereof, wherein the polygalacturonic acid compound is crosslinked with a divalent cation and the divalent cation concentration ranges from 0.5 to 2 g/l of the substrate. Learn More

A U.S. Patent Application for a "Sensor Interface for Single Use Containers" was published by Emerson on June 28th, 2018. The present invention is a single-use sensor interface for coupling a single-use container to a re-usable sensing instrument is provided. The single-use sensor interface includes a polymeric flange couplable to a wall of the single-use container. The polymeric flange has a sidewall and an interface portion configured to receive a deflectable polymeric diaphragm. A deflectable polymeric diaphragm is coupled to the interface portion of the polymeric flange. An instrument coupling portion is sealingly coupled to the polymeric flange... Learn More

A U.S. Patent for "Vessel Closures and Methods for Using and Manufacturing Same" was awarded to Sartorius Stedim on June 26th, 2018. The present invention is a vessel closure comprising: a body, one or more apertures extending axially through the body, one or more inserts extending axially through the one or more apertures, and a cast seal disposed within the body and surrounding each insert, wherein at least one of the one or more inserts comprises silicone tubing, at least a portion of the silicone tubing being treated with a primer where the silicone tubing contacts the cast seal, the cast seal comprising a casting agent cured after the one or more inserts are inserted... Learn More

A U.S. Patent for "Recombinant Polypeptide Production"
was awarded to MedImmune on June 26th, 2018. The present invention is a method for producing a recombinant polypeptide in a mammalian cell culture in which the mammalian cells have a modified microRNA activity level. In one embodiment, a microRNA activity level is increased. In another embodiment, a microRNA activity level is decreased. In a more particular embodiment, the mammalian cells have a reduced miRNA-let-7a activity level. Learn More

A U.S. Patent Application for "Detection of Infectious Prion Protein by Seeded Conversion of Recombinant Prion Protein" was published by the U.S. Government on June 21st, 2018. The present invention relates to methods and compositions for the detection of infectious proteins or prions in samples, including the diagnosis of prion related diseases. One embodiment is an ultrasensitive method for detecting PrP-res (PrP.sup.Sc) that allows the use of recombinant PrP-sen (rPrP-sen) as a substrate for seeded polymerization. A sample is mixed with purified rPrP-sen to make a reaction mix which is incubated to permit aggregation of the rPrP-sen with the PrP-res that may be present... Learn More

A U.S. Patent Application for a "Reconfigurable Modular Processing Facility" was published by Fluor on June 21st, 2018. The present invention is a method for constructing a reconfigurable modular processing facility, the method comprising: assembling a plurality of vertical walls, wherein the spaces between the walls define a plurality of stations; assembling a plurality of horizontal levels, wherein the spaces between the levels and the walls define the plurality of stations; constructing a lift within a center portion of the facility, wherein the lift is configured to access each of the plurality of stations; assembling one or more pods sized to fit within the stations... Learn More