Bioprocess Alert

This video, which has 92,000+ views, utilizes a narrative coupled with animation to explain the principles of Raman Spectroscopy.

This video on Protein Purification provides insight into the process and tools that scientists and engineers use to explore proteins.

This video, which has 165,000+ views, provides an informative overview of the principles at work in four types of flowmeters; Mechanical, Magnetic, Vortex and Ultrasonic.

This video, which has 2,300+ views in its first 24 hours online, explains Ethernet/IP. It explains both what it is, and perhaps just as importantly, what it is not.

This video, which has 157,000+ views, explains the dissolution of a solute in a solution.

This video explains the principles and practices associated with aeration and agitation in bioprocessing applications.

This video, which has 2.4+ million views, delivers an informative view of what constitutes a cell.

This video, which has 320,000+ views, explains the process of anaerobic respiration.

This video, which has 15,000+ views, provides a summary of quantitative and qualitative validation cleaning techniques for FDA regulated applications. It includes a Q&A session that provides further insights.

This video, which has 684,000+ views, discusses the basics of steam hammer and its effects.

This video, which has 498,000+ views, discusses the basics of fluid cavitation and its effects.

This video, which has 99,000+ views, explains the structural components and functionalities of a pressure relief valve.

This video, which has 197,000+ views, provides an informal yet informative overview of amino acids.

Protein folding is the continual and universal process whereby the long, coiled strings of amino acids that make up proteins in all living things fold into more complex three-dimensional structures. By understanding how proteins fold, and what structures they are likely to assume in their final form, researchers are then able to move closer to predicting their function.

Baffles are internals, generally flat plates, used in agitated vessels to optimize and stabilize the mixing flow pattern and minimize variation in agitator power draw. Baffle recommendations are part of the agitator vendor's scope although the baffles are designed and fabricated by others. Proper baffle implementation dramatically impacts process results. Learn More

In deciding which single-use platform to choose, reliability and robustness are fundamental requirements. Beyond process performance, it is important to consider how control systems and automation come into play, how easy it is to implement the platform, and if the design is flexible enough to support varying production volumes.

This video, which has 85,000+ views, is the second in a series of three videos depicting the major stages of industrial-scale bioprocessing: fermentation, separation/recovery, and purification. This video describes the process by which a biological product—in this case, molecules of Green Fluorescent Protein (GFP)—is recovered from host e coli cells. The steps of this process are, in very basic terms: (1) separation of cell solids from the broth, (2) disruption of those host cells to release the product contained in them, and (3) isolation of the product through removal of cell debris and other impurities. Tools used to perform these steps include centrifuges, cell disrupters and microfilters.

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Original Publication Date:
10/10/2013

One of the primary safeguards that life science companies use to help prevent contamination in cleanroom operations is Gowning.
Gowning is basically putting on clothing -- in this case layers of specially designed garments, covers, masks and gloves -- that isolates your body from the clean room environment.

Gowning is more involved than just slipping on a coverall and going to work. Gowning is itself a process, with carefully sequenced steps to ensure that your body is fully covered, and that the protective garb remains free of contamination.

This video, which has 133,000+ views, demonstrates the top-down approach for both general and aseptic gowning.

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Original Publication Date:
04/05/2013

What is Cell Culture?
➜ Cell culture is an incredibly useful in vitro tool in cell biology research. In this technique, cells are removed from an organism and grown in suitable artificial conditions for study. Live cell culture enables the development of model systems for understanding physiological processes in the human body, mutagenesis, carcinogenesis, drug screening and development, and much more. In this video, which has 55,000+ views, we will give you an overview of the categories of cells (Primary vs. Established Cell Lines), how to establish suitable growth conditions for your cells, and the principles of cell culture lab practices.

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Original Publication Date:
11/13/2015

This video, which has 94,000+ views, is the third in a series of three videos depicting the major stages of industrial-scale fermentation: fermentation, separation/recovery, and purification. This program describes the process by which a biological product—in this case, a pure, concentrated solution of Green Fluorescent Protein (GFP)—is derived from clarified lysate. This is accomplished through multiple variations of two basic procedures: chromatography and filtration.

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Original Publication Date:
10/30/2013

In this week's Two Minute Tuesday video tutorial we will examine the impact of pH on cell culture in bioreactors. This video will detail the importance of pH for optimal cell growth within a bioreactor, what factors can impact pH and how to control the pH of the growth media during production.

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Original Publication Date:
07/18/2018

Understanding the impact of aeration and thus the dissolution of oxygen into the bioreactor growth media for uptake by the production cell line is paramount for success in bioprocessing. Watch the following Two Minute Tuesday technical training video for an overview of the concepts and important considerations for aeration in bioreactor operations.

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Original Publication Date:
07/18/2018

This video, which has 218,000+ views, describes the role of the fermentation process in the creation of biological products and illustrates commercial-scale fermentation at a cellular level. Included are descriptions of types of fermentation (intracellular, extracellular), types of cells (aerobic, anaerobic), and cell nutrition (media components).

The program follows the production of a batch of Green Fluorescent Protein (GFP) from preparation through harvesting, including growth of seed stock, scaling up, and life cycle growth pattern phases (lag, exponential/log, stationary, death). Featured throughout is the monitoring of various production process parameters, including time, temperature, pressure, pH, agitation, etc.

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Original Publication Date:
08/27/2013

This video, which has had 83,000+ views, delves into the principles and practices of affinity chromatography. Learn More

Original Publication Date:
02/03/2015

This video provides a brief overview of the history of cGMP guidelines from principles to practices.

This video, which has 40,000+ views, provides an informative explanation of the principles and practices associated with continuous culture.

This video, which has 50,000+ views, provides an informative explanation of the principles and practices associated with fed batch fermentation.

This video, which has 65,000+ views, provides an informative explanation of the principles and practices associated with batch culture.

This video, which has 118,000+ views, explains the principles and practices associated with centrifugation.

This video, which has 17,000+ views, talks about a typical industrial fermenter and its different parts with their role in fermentation.

After watching this video you will be able to better scrutinize your process, identify gaps and propose solutions relating to bioreactor contamination mitigation. You will also learn what questions to ask your vendor to make the optimal filter selection and you will be able to conduct an objective comparison of filter claims vs. performance.

This video, which has 8,500+ views, delves into the principle of chromatography in a light-hearted and entertaining way.

In this video, which has 19,000+ views, Gregory Sacha, Ph.D. of Baxter BioPharma Solutions provides an intro to lyophilization and the anatomy of a lyophilizer.

In this video, the presenter addresses a key element of upstream bioprocessing, lab culture and media prep.

This video, which has 15,000+ views, explains the principles and practices associated with the measurement of pressure.

In this video, which has 194,000+ views, Genentech explains how it is currently conducting research on how Apoptosis plays a central role in normal development and tissue homeostasis.

This video, which has 20,000+ views, presents a definition of Operational Excellence as one of three distinct strategies or value propositions an organization can choose from to compete and ultimately become a market leader in its market or industry.

This training video focuses on the basics of biologics development and outlines the common steps in a modern bioproduction process, particularly the cell culture or upstream unit operations. Learn More

This video, which has 15,000+ views, provides an overview of the various mixing technologies available and their associated functionality.

This video, which has 23,000+ views, provides a basic introduction to JMP.

This video, which has 278,000+ views, discusses and demonstrates the use of centrifugation, the force exerted by rotational motion, in order to separate out components of a mixture that have different weights.

This video, which has 279,000+ views, explains the principles and practices associated with potentiometric pH measurement.

This user-guided site tour of the Samsung Biologics facility provides unique insight into a state-of-the-art bioprocessing facility.