Simulation of pH Level Distribution Inside a Coiled Flow Inverter (CFI) for Continuous Low pH Viral Inactivation



The continuous production of monoclonal antibodies (mAb) with the help of disposable equipment poses one of the future major changes in the pharmaceutical industry. Consequently, also continuous viral clearance needs to be developed. The coiled flow inverter (CFI) was successfully implemented in the continuous downstream as a residence time module for low pH viral inactivation. As the elution profile of the upstream continuously operated Protein A chromatography results in fluctuating pH values, the pH level distribution inside the CFI is highly relevant. This work presents a detailed investigation of pH level distribution inside the CFI at varying inlet conditions with the help of computational fluid dynamics (CFD) simulation… Learn More