Process Design and Development of a Mammalian Cell Perfusion Culture in Shake-Tube and Benchtop Bioreactors



The development of mammalian cell perfusion cultures is still laborious and complex to perform due to the limited availability of scale-down models and limited knowledge of time- and cost-effective procedures. The maximum achievable viable cell density, minimum cell-specific perfusion rate, cellular growth characteristics, and resulting bleed rate at steady-state operation are key variables for the effective development of perfusion cultures. In this study, we developed a stepwise procedure to use shake tubes in combination with benchtop bioreactors for the design of a mammalian cell perfusion culture at high productivity and low product loss in the bleed for a given expression system. Learn More