A U.S. Patent Application for a “Chromatography Method” was published by GE on August 8th, 2019. The present invention is a method of making affinity chromatography beads suitable for purification of immunoglobulin containing proteins or parts thereof, from contaminants that are larger than immunoglobulin containing proteins, the method comprising: a) providing allylated chromatography beads having an allylated shell layer and an allylated inner core wrapped around by the allylated shell layer; b) inactivating the allylated shell layer of the beads to form a lid of hydrolyzed allyl groups having a thickness of 1-8 .mu.m; c) activating the allylated inner core of the beads. Learn More