A U.S. Patent for a “High-Cell Density Fed-Batch Fermentation Process for Producing Recombinant Protein” was awarded to Pfizer on May 28th, 2019. The present invention includes methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions for use in the methods of the present invention, are provided. Learn More