A U.S. Patent for a ” Cost-Effective Raman Probe Assembly for Single-Use Bioreactor Vessels”  was awarded to Kaiser Optical  on April 16th, 2019. The present invention consists of systems and methods used to couple an optical sampling probe to a port in a single-use bioreactor bag for in-process monitoring. A combination of re-useable and disposable components maintain precision while reducing costs. A disposable barb with an integral window, received by the port of the reaction vessel, is coupled to a re-useable optic component with a focusing lens. A separate focus alignment tool is used to set the lens position to a precise focal point before placement of the optic component into the barb. Learn More