We used Eppendorf packed-bed bioreactors filled with Fibra-Cel disks for the cultivation of Chinese Hamster Ovary (CHO) cells. The objective of this study was to compare process performance in BioBLU single-use and traditional glass packed-bed bioreactors. Alkaline phosphatase (ALKP)-secreting CHO cells were used to measure protein production in each bioreactor. Overall, the results from these comparisons suggest that there is no significant difference between the reusable and singleuse FibraCel basket systems for bench-scale production of recombinant proteins. Productivity of cells and collection of secreted proteins will not be hindered by the implementation of single-use bioreactor systems.

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Original Publication Date: 07/1/2012