Overexpression of N-Glycosylation Pathway Regulators to Modulate Glycosylation of Recombinant Proteins



A U.S. Patent for “Overexpression of N-Glycosylation Pathway Regulators to Modulate Glycosylation of Recombinant Proteins” was awarded to Amgen on March 12th, 2019. The present invention is a method of regulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture process, comprising: transfecting a mammalian host cell to overexpress a protein that is involved in an N-glycosylation pathway, wherein the protein involved in the N-glycosylation pathway is selected from the group…wherein the mammalian cell culture is perfused using alternating tangential flow (ATF), and wherein the recombinant protein is harvested and purified. Learn More