A Fully Automated Three-Step Protein Purification Procedure for Up to Five Samples Using the NGC Chromatography System

In recent years significant progress has been made in the miniaturization of protein expression and purification. As a consequence of miniaturization, the protein purification is restricted to a one-step process. In addition, the amount of purified protein is usually in the μg-range. This might be suitable if a sensitive initial screening assay is available. However, when larger amounts of proteins are required robotic platforms are no longer appropriate. To address this topic we have used the NGC chromatography system for automated purification of up to five samples using a three-step purification procedure. Learn More