Development of Capacitance Tools: At-line Method for Assessing Biomass of Mammalian Cell Culture and Fixed Cell Calibration Standard

Capacitance has been used to monitor “biomass” in cell culture and fermentation processes for several decades. The present work explored various ways to support GMP use and create robust methods that would assure proper performance of capacitance equipment. The first goal was to develop a method for measuring at-line capacitance that could serve to correct or confirm online measurements from bioreactors. With appropriate control of culture temperature, vessel geometry, mixing, and timing; an at-line system was developed that consistently predicted online capacitance outputs. The at-line method yielded values that differed systematically from online values… Learn More