A U.S. Patent for “Purification of Biological Molecules” was awarded to MilliporeSigma on December 15th, 2020. The present invention is a continuous process for the purification of a target molecule comprising the steps of: a) providing a sample comprising the target molecule and one or more impurities; b) adding at least one precipitant to the sample and removing one or more impurities, thereby to recover a clarified sample; c) subjecting the clarified sample from step (b) to a bind and elute chromatography step comprising at least two separation units, thereby to obtain an eluate comprising the target molecule; and d) subjecting the eluate to flow-through purification comprising use of two or more media, one of which is activated carbon and the other(s) are selected from anion-exchange chromatography media…
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