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Monitoring Cell Productivity for the Production of Recombinant Proteins by Flow Cytometry: An Effective Application Using the Cold Capture Assay | Aspen Xchange
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Monitoring Cell Productivity for the Production of Recombinant Proteins by Flow Cytometry: An Effective Application Using the Cold Capture Assay



In this study, the productivity of Chinese Hamster Ovary (CHO) cells expressing a monoclonal antibody was analyzed over the course of the cultivation process. In addition to calculating the specific cell productivity based on the traditional product titer determined by HPLC analysis, culture productivity of single cells was also analyzed via flow cytometry using a cold capture assay. The cold capture assay is a cell surface labelling technique described by Brezinsky et al., which allows for the visualization of a product on the surface of the producing cell. The cell productivity results obtained via HPLC and the results of cold capture assay remained in great accordance over the whole cultivation process. Accordingly, our study demonstrates that the cold capture assay offers an interesting, comparatively time‐effective, and potentially cheaper alternative for monitoring the productivity of a cell culture.
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