Many shaken bioprocesses progress from a small scale (e.g. microtiter plate) to a relatively large scale (e.g. 2,500L bioreactors). Once a culture has been optimized for a microtiter plate (MTP), it can be scaled into larger vessels using a variety of parameters (e.g. OTRmax); however, the initial shaking conditions for a micro-titer plate are not always known. Here we discuss the key equations and relationships that can be used to determine the initial conditions for a shaken bioprocess in an MTP and illustrate an example in a 96-well plate.