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Virus Purification | Aspen Xchange
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A U.S. Patent for “Virus Purification” was awarded to Kentucky Bioprocessing on November 3rd, 2020. The present invention is a method for purifying at least one virus harvested from a source organism, comprising: removing cellular debris from the virus by passing the at least one virus through a tangential flow filtration apparatus comprising a porous ceramic filter; concentrating the virus; and performing separations comprising sub-steps of separating host cell contaminants from the virus, subjecting the virus to affinity chromatography to elute the virus, and subjecting the virus to multi-modal chromatography to separate residual impurities from the virus based on size differences between the impurities as retentate and the virus as permeate. 
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