This guide describes the detailed procedure of Pichia pastoris fermentation in a BioBLU® 3f Single-Use Bioreactor controlled by a BioFlo® 320 bioprocess control system. We ran a fed-batch fermentation and started feed- ing upon the appearance of a significant dissolved oxygen spike, which indicated carbon source depletion. Finally, based on this fermentation strategy, a reasonably high- density P. pastoris culture was obtained in the single-use vessel. This guide can be very helpful for those who are new to P. pastoris fermentation in bioreactors.
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