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Recognizing the Potential Impact of Agitation Directionality On Oxygen Mass Transfer Through a kLa Evaluation Within the BIOne Single-Use Bioreactor | Aspen Xchange
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Recognizing the Potential Impact of Agitation Directionality On Oxygen Mass Transfer Through a kLa Evaluation Within the BIOne Single-Use Bioreactor



Defining a suitable agitation strategy is essential for the successful development of upstream mammalian bioprocesses. Such a strategy can drive oxygen mass transfer within the bio- reactor system to effectively support the aerobic needs of the expanding culture. An inadequate agitation strategy can result in the emergence of chemical gradients within the system. The existence of such nonuniformity within a bioreactor can result in extracellular conditions which may be unfavorable to the overall growth of the cell culture. Hypoxic conditions in a bioreactor are defined by low dissolved oxygen concentrations.
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