The purification and manufacture of recombinant proteins, especially those lacking affinity handles, often requires multiple chromatography steps and complicated feedstream conditioning, resulting in high production costs and relatively low recovery. These issues may be exacerbated by low protein expression, pH sensitivity, and limited stability. Mixed-mode chromatography resins have become an important tool for the purification of such proteins. The objective of this study was to purify one such recombinant protein, EPA, from E. coli fermentate to >95% purity and recovery. Learn More