Warning: include_once(/home/aspenxadmin/public_html/files/EIT_html_excerpt.php): failed to open stream: No such file or directory in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-1.php on line 5

Warning: include_once(): Failed opening '/home/aspenxadmin/public_html/files/EIT_html_excerpt.php' for inclusion (include_path='.:/opt/alt/php72/usr/share/pear') in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-1.php on line 5

Warning: include_once(/home/aspenxadmin/public_html/files/EIT_html_excerpt.php): failed to open stream: No such file or directory in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-2.php on line 5

Warning: include_once(): Failed opening '/home/aspenxadmin/public_html/files/EIT_html_excerpt.php' for inclusion (include_path='.:/opt/alt/php72/usr/share/pear') in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-2.php on line 5

Warning: include_once(/home/aspenxadmin/public_html/files/EIT_html_excerpt.php): failed to open stream: No such file or directory in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-3.php on line 5

Warning: include_once(): Failed opening '/home/aspenxadmin/public_html/files/EIT_html_excerpt.php' for inclusion (include_path='.:/opt/alt/php72/usr/share/pear') in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-3.php on line 5

Warning: include_once(/home/aspenxadmin/public_html/files/EIT_html_excerpt.php): failed to open stream: No such file or directory in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-4.php on line 5

Warning: include_once(): Failed opening '/home/aspenxadmin/public_html/files/EIT_html_excerpt.php' for inclusion (include_path='.:/opt/alt/php72/usr/share/pear') in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-4.php on line 5

Warning: include_once(/home/aspenxadmin/public_html/files/EIT_html_excerpt.php): failed to open stream: No such file or directory in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-5.php on line 5

Warning: include_once(): Failed opening '/home/aspenxadmin/public_html/files/EIT_html_excerpt.php' for inclusion (include_path='.:/opt/alt/php72/usr/share/pear') in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-5.php on line 5

Warning: include_once(/home/aspenxadmin/public_html/files/EIT_html_excerpt.php): failed to open stream: No such file or directory in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-6.php on line 5

Warning: include_once(): Failed opening '/home/aspenxadmin/public_html/files/EIT_html_excerpt.php' for inclusion (include_path='.:/opt/alt/php72/usr/share/pear') in /home/aspenxadmin/public_html/wp-content/themes/magzilla/framework/vc_shortcodes/module-6.php on line 5

Warning: in_array() expects parameter 2 to be array, string given in /home/aspenxadmin/public_html/wp-content/plugins/ajax-search-pro/includes/classes/actions/class-asp-indextable.php on line 156

Warning: get_class() expects parameter 1 to be object, null given in /home/aspenxadmin/public_html/wp-content/plugins/real-media-library/inc/comp/WPML.class.php on line 32
Raman-Based Dynamic Feeding Strategies Using Real-Time Glucose Concentration Monitoring System During Adalimumab Producing CHO Cell Cultivation | Aspen Xchange
Warning: Use of undefined constant full - assumed 'full' (this will throw an Error in a future version of PHP) in /home/aspenxadmin/public_html/wp-content/themes/magzilla/header.php on line 64

Raman-Based Dynamic Feeding Strategies Using Real-Time Glucose Concentration Monitoring System During Adalimumab Producing CHO Cell Cultivation



The use of Process Analytical Technology tools coupled with chemometrics has been shown great potential for better understanding and control of mammalian cell cultivations through real-time process monitoring. In-line Raman spectroscopy was utilized to determine the glucose concentration of the complex bioreactor culture medium ensuring real-time information for our process control system.

This work demonstrates a simple and fast method to achieve a robust partial least squares calibration model under laboratory conditions in an early phase of the development utilizing shake flask and bioreactor cultures. Two types of dynamic feeding strategies were accomplished where the multi-component feed medium additions were controlled manually and automatically based on the Raman monitored glucose concentration. Learn More