A U.S. Patent for “Method for Producing a Recombinant Protein on a Manufacturing Scale” was awarded to Boehringer Ingelheim on August 25th, 2020. The present invention is a method for plasmid-free production of a heterologous protein of interest, comprising the steps of a) cultivating bacterial expression host cells in a culture medium, said cultivating comprising a mode that employs adding a feeding medium, wherein said mode is selected from fed-batch mode, semi-continuous mode, and continuous mode, wherein (i) one copy of a DNA construct is integrated in the chromosome of the host cells, and wherein said DNA construct consists… Learn More