Optimal Bioreactor Inoculum Preparation in Shake Flasks with Automated Seed Inoculation, Conditioning and Online Monitoring

While monitoring and control of inoculum characteristics at the seed fermenter stage are well established in most bioprocesses, the very first steps of culture expansion in shake flasks are usually neglected, even though they are influencing all following process steps. Especially for organisms with complex metabolic regulation or morphological variability, the utilization of suboptimal inocula can severely worsen the bioprocess outcome.
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