For the production of biopharmaceuticals, a procedure called seed train or inoculum train is required to generate an adequate number of cells for the inoculation of the production bioreactor. This seed train is time- and cost-intensive but offers potential for optimization. A method and a protocol are described for seed train mapping, directed modeling, and simulation as well as its optimization regarding selected optimization criteria such as optimal points in time for cell passaging. Furthermore, the method can also be applied for the transfer of a seed train to a different production plant or the design of a new seed train, for example, for a new cell line. Learn More