Design of Experiment (DoE) Approach to Identify Critical Parameters in a Counterflow Centrifugation System

Counterflow centrifugal (CFC) systems have been applied to cell manufacturing for the processes of cell selection and buffer exchange. The recently developed Rotea™ (Scinogy) is a CFC device that enables closed automated buffer exchange for small-scale cell manufacturing. However, the fluid dynamics within the processing chamber is complex and are influenced by a number of factors, including flowrate, centrifugal force, and buffer density. The impact of individual or compounded effects on cell quality is unclear. This study aims to determine the critical factors for mesenchymal stem cell (MSC) processing using the Rotea™. Learn More