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Real-Time Monitoring and Control of CHO Cell Apoptosis by In Situ Multifrequency Scanning Dielectric Spectroscopy | Aspen Xchange
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Real-Time Monitoring and Control of CHO Cell Apoptosis by In Situ Multifrequency Scanning Dielectric Spectroscopy



Process control strategies based on the physiological status of cells have recently been used to enhance mammalian cell culture productivity and robustness. In this study, we investigated the feasibility of using full-spectrum dielectric spectroscopy for detecting shifts in cell physiology and as a feedback tool to increase process efficiency. Multi-frequency permittivity spectra were collected from cell culture processes in which apoptosis was induced by glucose depletion, nutrient depletion, or chemical treatment. Meanwhile, key parameters of critical frequency (fc) and Cole-Cole alpha (α) were calculated in real time from the β-dispersion curve and correlated to data from off-line viability measurements. Results show that physiological changes in apoptotic cells were reflected in the on-line parameters earlier than from off-line methods. Learn More