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Use of Caprylic Acid Precipitation for Protein Purification | Aspen Xchange
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A U.S. Patent for “Use of Caprylic Acid Precipitation for Protein Purification” was awarded to Bristol Myers Squibb on August 11th, 2020. The present invention is a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, comprising: a) subjecting the mixture to a first chromatography step, wherein the first chromatography is a protein A affinity chromatography; b) recovering the protein of interest in an elution solution having a low pH between 2.5 and 4; c) adding caprylic acid to the elution solution and then adjusting the pH to at least 5.5 to form a contaminant precipitate… Learn More