A U.S. Patent for “Coated Cell Culture Apparatus and Methods of Use” was awarded to Worcester Polytechnic Institute on August 4th, 2020. The present invention is a device, and methods of using or making the device, for engineering cells in vitro are disclosed. In some aspects, a cell culture device comprises at least one glass or polymer surface configured for incubating cells in a culture medium; a charged molecule electrostatically bound to the surface; and a polyelectrolyte multilayer (PEM) electrostatically bound to the charged molecule, the PEM comprising one or more bi-layers of oppositely charged polyelectrolytes, and the PEM having a sufficient thickness… Learn More