A U.S. Patent for “Protein Quantification by Near Infrared Spectral Imaging” was awarded to Temple University on July 14th, 2020. The present invention is a method of quantifying protein in an electrophoresis gel, comprising the steps of: loading a sample including at least one protein into a gel via electrophoresis to generate at least one protein band; acquiring an infrared spectral dataset from the at least one protein band using near infrared spectral imaging (NIRSI); generating an infrared hyperspectral image from the infrared spectral dataset for each of the at least one protein band, the infrared hyperspectral image comprising a plurality of pixels.. Learn More