Small-Scale Bioreactor Supports High Density HEK293 Cell Perfusion Culture for the Production of Recombinant Erythropoietin



Process intensification in mammalian cell culture-based recombinant protein production has been achieved by high cell density perfusion in recent years. As the majority of therapeutic proteins are produced in Chinese Hamster Ovary (CHO) cells, intensified perfusion processes have been mainly developed for this type of host cell line. However, the use of CHO cells can result in non-human posttranslational modifications of the protein of interest, which may be disadvantageous compared with human cell lines. In this study, we developed a high cell density perfusion process of Human Embryonic Kidney (HEK293) cells producing recombinant human Erythropoietin (rhEPO). Learn More