Testing of Online Optical Cell Biomass Probe Linearity and Accuracy Across Changing Process Conditions



A long-standing challenge for on-line optical cell biomass probes has been to maintain biomass prediction accuracy across changing process conditions, particularly for organisms requiring highly variable rates of agitation and aeration in order to avoid oxygen-limited growth. For microbial organisms grown to high cell biomass densities, being able to accurately track the biomass across the entire growth cycle from inoculation to harvest has been difficult to achieve due to the limited linearity range of traditional on-line methods and variation in sensor readings with changing process conditions. A comparison study of the linear range and the effects of changing agitation and aeration conditions on biomass prediction error for a methanotrophic culture are compared for two commercially available on-line optical reflectance probes. Learn More