Development of an Intensified Fed-Batch Production Platform with Doubled Titers Using N-1 Perfusion Seed for Cell Culture Manufacturing

The goal of cell culture process intensification is to increase volumetric productivity, generally by increasing viable cell density (VCD), cell specific productivity or production bioreactor utilization in manufacturing. In our previous study, process intensification in fed-batch production with higher titer or shorter duration was demonstrated by increasing the inoculation seeding density in combination with media enrichment. In this study, we further increased seeding density using perfusion N-1 seed cultures, which increased titers already at industrially relevant levels by 100% in 10-14 day bioreactor durations for four different mAb-expressing CHO cell lines. Learn More