Single-use plastic culture devices are commonly used for two-dimensional (2-D) cell cultures. However, as oxygen is generally supplied by uni-directional diffusion from the top gas-liquid interface, 2-D cell cultures in the absence of medium flow may experience rapid exhaustion of dissolved oxygen at the bottom of culture wares with high cell densities. To overcome this issue, special cell culture devices utilizing the gas-permeable membrane at the bottom have been developed. In this study, cell growth, metabolic activity, and productivity were evaluated in gas-permeable culture devices compared to those from conventional gas-impermeable culture devices. Learn More