A U.S. Patent Application for a “Novel Method of Protein Purification” was published by Ansun Biopharma on May 14th, 2020. The present invention is a composition comprising a polypeptide comprising the amino acid sequence of SEQ ID NO: 1 and 2 made by the method comprising: (a) providing a culture of a gram negative bacterial cell expressing the intracellular protein of interest; (b1) contacting the culture with an inorganic salt; holding the culture containing the added inorganic salt for at least 10 minutes; contacting the culture containing the added inorganic salt with a chelating agent; or (b2) contacting the culture with an inorganic salt… Learn More