A U.S. Patent for “Methods of Controlling the Formation of Disulfide Bonds in Protein Solutions” was awarded to Genzyme on May 12th, 2020. The present invention is a method of controlling the number of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess, the method comprising: (a) contacting the polypeptides with a conditioned solution at a specific time point during the bioprocess, wherein the conditioned solution comprises a redox reagent, and (b) incubating the conditioned solution comprising the polypeptides for a predetermined time at a predetermined temperature; wherein the incubation of the polypeptides with the conditioned solution controls the formation of disulfide bonds between the polypeptides of the protein; wherein the number of disulfide bonds between the polypeptides of the protein is increased… Learn More