This handbook, created by Cytiva, describes the use of size exclusion chromatography (SEC) for the purification and separation of biomolecules, with focus on practical information for optimized results. Since the introduction of the first SEC resin, Sephadex in 1959, SEC has played a key role in the purification of proteins and enzymes, polysaccharides, nucleic acids, and other biological macromolecules. Learn More