In recent years, adoptive T-cell therapy (ACT) has emerged as a promising way to treat systemic cancers such as Acute Lymphoblastic Leukemia. However, robustness and reproducibility of the manufacturing process remain challenging and it is therefore pivotal to understand the effect that cell culture conditions have on the expansion and differentiation of T-cells. This work investigates the effect of CO2 on the expansion of T-cells using the bioreactor AppliFlex ST due the advantages of this single-use system such as lower initial investments, faster setup and reduced cross-contamination risks. The influence of CO2 is of particular interest with regards to potential allogeneic T-cell therapies and the associated cultivation at the large scale, where CO2-removal may become insufficient. Learn More