A U.S. Patent for “pH Adjustment to Improve Thaw Recovery of Cell Banks” was awarded to Genentech on March 31st, 2020. The present invention is a method of improving thaw recovery of cell banks comprising freezing CHO cells for banking in a freezing medium, wherein the freezing medium comprises a buffered solution and a cryoprotective agent, and wherein the freezing medium has a pH of 7.7 to 8.3 prior to freezing. Learn More