A U.S. Patent for “Isolating Cells Expressing Secreted Proteins” was awarded to Regeneron on March 24th, 2020. The present invention is a method of high expression of a protein of interest, comprising: (a) transfecting a mammalian cell with (i) a nucleic acid encoding the protein of interest and (ii) a nucleic acid encoding a cell surface capture molecule that comprises a membrane anchor and is capable of binding the protein of interest; (b) culturing the mammalian cell under conditions in which the protein of interest and cell surface capture molecule are expressed, and a protein of interest-cell surface capture molecule complex is formed and displayed on the cell surface of the mammalian cell… Learn More