A U.S. Patent Application for “Methods for Increasing Mannose Content of Recombinant Proteins” was published by Amgen on March 19th, 2020. The present invention is a method for modulating mannose 5 on a recombinant protein during a mammalian cell culture process comprising: establishing a cell culture of mammalian cells in a cell culture medium, wherein the mammalian cells produce a recombinant protein; limiting the amount of glucose in the cell culture medium, wherein the concentration of the glucose is from about 0 to 6 g/L; and supplementing the cell culture medium with galactose or sucrose… Learn More