A U.S. Patent Application for “Microcarrier Perfusion Culturing Methods and Uses Thereof” was published by Genzymeon March 12th, 2020. The present invention is a method for testing a manufacturing process for making a recombinant protein, the method comprising: providing a shake tube containing an adherent Chinese hamster ovary (CHO) cell containing a nucleic acid encoding a recombinant protein disposed in a first liquid culture medium, wherein the first liquid culture medium occupies about 10% to about 30% of the volume of the shake tube and contains a plurality of microcarriers at a concentration of about 1.0 g/L to about 15.0 g/L… Learn More