A U.S. Patent for “Compositions and Methods for Protein Glycosylation” was awarded to GSK on March 3rd, 2020. The present invention is oligosaccharyl transferases for use in N-glycosylating proteins of interest in vitro and in host cells. Methods for using such oligosaccharyl transferases, nucleic acids encoding such oligosaccharyl transferases, and host cells comprising such oligosaccharyl transferases are also provided herein. Glycoconjugates generated by using such oligosaccharyl transferases are also provided herein. Learn More