A U.S. Patent for “Cross-Scale Modeling of Bioreactor Cultures Using Raman Spectroscopy” was awarded to Biogen on February 18th, 2020. The present invention is a method of assessing a bioreactor culture, the method comprising: (i) obtaining a Raman spectrum of a manufacturing-scale bioreactor culture using a probe configured inline with the bioreactor; and (ii) determining a culture parameter of the manufacturing-scale bioreactor culture using a multivariate partial least squares (PLS) model that relates the Raman spectrum to the culture parameter, wherein the model was developed based on one or more test bioreactor cultures… Learn More