A U.S. Patent for “Methods of Replicating a Large Scale Eculizumab Production Cell Culture” was awarded to Alexion on January 28th, 2020. The present invention is a method of replicating a large-scale eculizumab production cell culture in a small scale culture, the method comprising: adding 3.7 mL of a first lipid solution containing linoleic acid, oleic acid and cholesterol prior to inoculation; providing a 4-L to 10-L fed batch bioreactor containing a first culture medium that occupies between about 70% and about 90% of the capacity of the bioreactor and has an initial cell density of between about 2.5.times.10.sup.5 and about 7.5.times.10.sup.5 NS0 cells/mL… Learn More