A U.S. Patent for a “Fed-Batch Process for the Production of Bacterial Ghosts” was awarded to Bird-C on January 7th, 2020. The present invention is a method for producing a bacterial ghost preparation comprising the steps: (a) providing bacterial cells comprising a lytic gene encoding a protein capable of forming a tunnel structure in the bacterial cell envelope wherein the expression of the lytic gene is under operative control of a regulatable promoter, (b) cultivating the bacterial cells under conditions wherein the lytic gene is not expressed to a final biomass concentration of about 10-80 g dry cell weight (DCW)/L, (c) after step (b)… Learn More