A U.S. Patent for the “In Vivo Production of Proteins” was awarded to Moderna Therapeutics on December 3rd, 2019. The present invention is a method of expressing a secreted protein in a mammalian subject, the method comprising administering a pharmaceutical composition comprising a plurality of lipid nanoparticles encapsulating a polynucleotide, wherein the lipid nanoparticle comprises a biodegradable cationic lipid, a neutral lipid, cholesterol, and a PEGylated lipid and the plurality of lipid nanoparticles has a mean particle size of between 80 nm to 160 nm, and wherein the polynucleotide comprises: (a) an open reading frame encoding the secreted protein… Learn More