Method for Using Light Scattering in Real Time to Directly Monitor and Control Impurity Removal in Purification Processes



A U.S. Patent for “Method for Using Light Scattering in Real Time to Directly Monitor and Control Impurity Removal in Purification Processes” was awarded to Amgen on November 19th, 2019. The present invention is a methodfor controlling levels of biological impurities in an elution pool from a cation exchange chromatography resin comprising: loading a mixture containing a recombinant protein and biological impurities on to a cation exchange chromatography resin; eluting by a salt gradient the recombinant protein from the cation exchange chromatography resin; measuring the intensity of light scattered by the eluate with a laser light. Learn More