A U.S. Patent for “Methods for Suspension Cultures of Human Pluripotent Stem Cells” was awarded to Janssen on August 13th, 2019. The present invention is an in vitro method of producing three-dimensional cell clusters of pluripotent stem cells comprising the steps of: a. treating pluripotent stem cells cultured in a planar adherent culture with a chelating agent or enzyme to release cell aggregates from the planar adherent culture; b. suspending the cell aggregates from the planar adherent culture in a culture medium in the presence of a Rho-kinase inhibitor without centrifuging the cell aggregates and without dissociating the cell aggregates to single cells. Learn More